Acoustic Wave (TSM) Biosensors: Weighing Bacteria

  • Eric Olsen
  • Arnold Vainrub
  • Vitaly Vodyanoy


This chapter is focused on the development and use of acoustic wave biosensor platforms for the detection of bacteria, specifically those based on the thickness shear mode (TSM) resonator. We demonstrated the mechanical and electrical implications of bacterial positioning at the solid-liquid interface of a TSM biosensor and presented a model of the TSM with bacteria attached operating as coupled oscillators. The experiments and model provide an understanding of the nature of the signals produced by acoustic wave devices when they are used for testing bacteria. The paradox of “negative mass” could be a real threat to the interpretation of experimental results related to the detection of bacteria. The knowledge of the true nature of “negative mass” linked to the strength of bacteria attachment will contribute significantly to our understanding of the results of “weighing bacteria.” The results of this work can be used for bacterial detection and control of processes of bacterial settlement, bacterial colonization, biofilm formation, and bacterial infection in which bacterial attachment plays a role.


Acoustic Wave Quartz Crystal Microbalance Sensor Surface Filamentous Phage Quartz Resonator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Aberl F and Wolf H (1993) Present trends in immunosensors. LaborPraxis 70–74, 76–77Google Scholar
  2. Ahluwalia A, Derossi D, Ristori C, Schirone A and Serra G (1992) A comparative study of protein immobilization techniques for optical immunosensors. Biosens. Bioelectron. 7:207–214Google Scholar
  3. Ahmad A and Ahmad S (1996) Solvent effect on antibody antigen interaction. Environ. Res. 5:29–36Google Scholar
  4. Aisenbrey C, Harzer U, Bauer-Manz G, Bar G, Chotimah INH, Bertani P, Sizun C, Kuhn A and Bechinger B (2006) Proton-decoupled 15N and 31P solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers. FEBS Journal 273:817–828Google Scholar
  5. Aizawa H, Kurosawa S, Tanaka M, Yoshimoto M, Miyake J and Tanaka H (2001) Rapid diagnosis of Treponema pallidum in serum using latex piezoelectric immunoassay. Anal. Chim. Acta 437:167–169Google Scholar
  6. Alberts B, Bray D, Lewis J, Raff M, Roberts K and Watson JD (1994) Molecular biology of the cell, 3rd ed. New York: Garland PublishingGoogle Scholar
  7. Auner GW, Shreve G, Ying H, Newaz G, Hughes C and Xu J (2003) Dual-mode acoustic wave biosensors microarrays. Proc. SPIE Int. Soc. Opt. Eng. 5119:129–139Google Scholar
  8. Babacan S, Pivarnik P, Letcher S., and Rand, A.G. 2000. Evaluation of antibody immobilization methods for piezoelectric biosensor application. Biosens. Bioelectron. 15:615–621Google Scholar
  9. Babacan, S., Pivarnik, P, Letcher S and Rand AG (2002) Piezoelectric flow injector analysis biosensor for the detection of Salmonella typhimurium. J. Food Sci. 61: 314–320Google Scholar
  10. Bailey CA, Fiebor B, Yen W, Vodyanoy V, Cernosek RW and Chin BA (2002) Thickness shear mode (TSM) resonators used for biosensing. Proc. SPIE Int. Soc. Opt. Eng. 4575:138–149Google Scholar
  11. Balasubramanian S, Sorokulova IB, Vodyanoy VJ and Simonian AL (2007) Lytic phage as a specific and selective probe for detection of Staphylococcus aureus—A surface plasmon resonance spectroscopic study. Biosens. Bioelectron. 22:948–955Google Scholar
  12. Ballantine DS, White RM, Martin SJ, Ricco AJ, Zellers ET, Frye GC, Wohltjen H, Levy M and Stern R (1997) Acoustic Wave Sensors: Theory, Design, & Physico-Chemical Applications. Academic Press, San DiegoGoogle Scholar
  13. Bandey HL, Martin SJ, Cernosek RW and Hillman AR (1999) Modeling the responses of thickness-shear mode resonators under various loading conditions. Anal. Chem. 71:2205–2214Google Scholar
  14. Bao L, Deng L, Nie L, Yao S and Wei W (1996a) Determination of microorganisms with a quartz crystal microbalance sensor. Anal. Chim. Acta 319:97–101Google Scholar
  15. Bao L, Deng L, Nie L, Yao S and Wei W (1996b) A rapid method for determination of Proteus vulgaris with a piezoelectric quartz crystal sensor coated with a thin liquid film. Biosens. Bioelectron. 11:1193–1198Google Scholar
  16. Barraud A, Perrot H, Billard V, Martelet C and Therasse J (1993) Study of immunoglobulin G thin layers obtained by the Langmuir-Blodgett method: application to immunosensors. Biosens. Bioelectron. 8:39–48Google Scholar
  17. Bashtovyy D, Marsh D, Hemminga MA and Pali T (2001) Constrained modeling of spin-labeled major coat protein mutants from M13 bacteriophage in a phospholipid bilayer. Protein Sci. 10:979–987Google Scholar
  18. Ben-Dov I, Willner I and Zisman E (1997) Piezoelectric immunosensors for urine specimens of Chlamydia trachomatis employing quartz crystal microbalance microgravimetric analyses. Anal. Chem. 69:3506–3512Google Scholar
  19. Berg S, Johannsmann D and Ruths M (2002) Frequency response of quartz crystal shear-resonator during an adhesive, elastic contact in a surface forces apparatus. J. Appl. Phys. 92:6905–6910Google Scholar
  20. Berkenpas E, Millard P and Pereira da Cunha M (2006) Detection of Escherichia coli O157:H7 with langasite pure shear horizontal surface acoustic wave sensors. Biosens. Bioelectron. 21:2255–2262Google Scholar
  21. Bessueille F, Dugas V, Vikulov V, Cloarec JP, Souteyrand E and Martin JR (2005) Assessment of porous silicon substrate for well-characterized sensitive DNA chip implement. Biosens. Bioelectron. 21: 908–916Google Scholar
  22. Betageri GV, Black CD, Szebeni J, Wahl LM and Weinstein JN (1993) Fc-receptor-mediated targeting of antibody-bearing liposomes containing dideoxycytidine triphosphate to human monocyte/macrophages. J. Pharm. Pharamcol. 45:48–53Google Scholar
  23. Borovskya B, Krim J, Syed Asif S and Wahl K (2001) Measuring nanomechanical properties of a dynamic contact using an indenter probe and quartz crystal microbalance. J. Appl. Phys. 90:6391–6396Google Scholar
  24. Branch DW and Brozik SM (2004) Low-level detection of a Bacillus anthracis simulant using love-wave biosensors on 36ˆ YX LiTaO3. Biosens. Bioelectron. 19:849–859Google Scholar
  25. Bunde RL, Jarvi EJ and Rosentreter JJ (1998) Piezoelectric quartz crystal biosensors. Talanta 46:1223–1236Google Scholar
  26. Buzby JC and Roberts T (1997) Economic costs and trade impacts of microbial food-borne illness. World Health Stat. Q. 50:57–66Google Scholar
  27. Bykov VA (1996) Langmuir-Blodgett films and nanotechnology. Biosens. Bioelectron. 11:923–932Google Scholar
  28. Carter RM, Jacobs MB, Lubrano GJ and Guilbault GG (1995a) Piezoelectric detection of ricin and affinity-purified goat anti-ricin antibody. Anal. Lett. 28:1379–1386Google Scholar
  29. Carter RM, Mekalanos JJ, Jacobs MB, Lubrano GJ and Guilbault GG (1995b) Quartz crystal microbalance detection of Vibrio cholerae O139 serotype. J. Immunol. Methods 187:121–125Google Scholar
  30. Casalinuovo I, Di Pierro D, Bruno E, Di Francesco P and Coletta M (2005) Experimental use of a new surface acoustic wave sensor for the rapid identification of bacteria and yeasts. Lett. Appl. Microbiol. 42:24–29Google Scholar
  31. Cavicacute BA, Hayward GL and Thompson M (1999) Acoustic waves and the study of biochemical macromolecules and cells at the sensor-liquid interface. Analyst 124:1405–1420Google Scholar
  32. Chang K-S, Jang H-D, Lee C-F, Lee Y-G, Yuan C-J and Lee S-H (2006) Series quartz crystal sensor for remote bacteria population monitoring in raw milk via the Internet. Biosens. Bioelectron. 21:1581–1590Google Scholar
  33. Chen M, Liu M, Yu L, Cai G, Chen Q, Wu R, Wang F, Zhang B, Jiang T and Fu W (2005) Construction of a novel peptide nucleic acid piezoelectric gene sensor microarray detection system. J. Nanosci. Nanotechnol. 5:1266–1272Google Scholar
  34. Chin RC, Salazar N, Mayo MW, Villavicencio V, Taylor RB, Chambers JP and Valdes JJ (1996) Development of a bacteriophage displayed peptide library and biosensor. Proc. SPIE Int. Soc. Opt. Eng. 2680:16–26Google Scholar
  35. Chothia C and Janin J (1975) Principles of protein-protein recognition. Nature 256:705–708Google Scholar
  36. Chou PY and Fasman GD (1974) Prediction of protein conformation. Biochemistry 13:222–245Google Scholar
  37. Clark LCJ and Lyons C (1962) Electrode systems for continuous monitoring in cardiovascular surgery. Ann. N. Y. Acad. Sci. 102:29–45Google Scholar
  38. Cornell BA, Braach-Maksvytis VL, King LG, Osman PD, Raguse B, Wieczorek L and Pace RJ (1997) A biosensor that uses ion-channel switches. Nature 387:580–583Google Scholar
  39. Cornell BA, Krishna G, Osman PD, Pace RD and Wieczorek L (2001) Tethered-bilayer lipid membranes as a support for membrane-active peptides. Biochem. Soc. Trans. 29:613–617Google Scholar
  40. Curie J and Curie P (1880) Ann. de Chim. et Phys. 91:294Google Scholar
  41. Dai J, Baker GL and Bruening ML (2006) Use of porous membranes modified with polyelectrolyte multilayers as substrates for protein arrays with low nonspecific adsorption. Anal. Chem. 78:135–140Google Scholar
  42. Davies LT and Rideal EK (1963) Interfacial Phenomena. Academic Press, New YorkGoogle Scholar
  43. Deisingh A and Thompson M (2001) Sequences of E. coli O157:H7 detected by a PCR-acoustic wave sensor combination. Analyst 126:2153–2158Google Scholar
  44. Deng L, Tan, H, Xu Y, Nie L and Yao S (1997) On-line rapid detection of urease-producing bacteria with a novel bulk acoustic wave ammonia sensor. Enzyme Microb. Technol. 21:258–264Google Scholar
  45. Deobagkar DD, Limaye V, Sinha S and Yadava RDS (2005) Acoustic wave immunosensing of Escherichia coli in water. Sens. Actuators B 104:85–89Google Scholar
  46. Dickert F, Hayden O, Lieberzeit P, Palfinger C, Pickert D, Wolff U and Scholl G (2003) Borderline applications of QCM-devices: synthetic antibodies for analytes in both nm- and um-dimensions. Sens. Actuators B 95:20–24Google Scholar
  47. Dickert FL, Hayden O, Bindeus R, Mann K, Blaas D and Waigmann E (2004) Bioimprinted QCM sensors for virus detection–screening of plant sap. Anal. Bioanal. Chem. 378:1929–1934Google Scholar
  48. Dickert FL, Lieberzeit P and Hayden O (2003) Sensor strategies for microorganism detection–from physical principles to imprinting procedures. Anal. Bioanal. Chem. 377:540–549Google Scholar
  49. Dybwad G (1985) A sensitive new method for the determination of adhesive bonding between a particle and a substrate. J. Appl. Phys. 58:2789–2790Google Scholar
  50. Eun A, Huang L, Chew F, Li S and Wong S (2002) Detection of two orchid viruses using quartz crystal microbalance (QCM) immunosensors. J. Virol. Methods 99:71–79Google Scholar
  51. Fan H, Lu Y, Stump A, Reed ST, Baer T, Schunk R, Perez-Luna V, Lopez GP and Brinker CJ (2000) Rapid prototyping of patterned functional nanostructures. Nature 405:56–60Google Scholar
  52. World Health Organization (1997) Foodborne diseases—possibly 350 times more frequent than reported. Need publication data here – also move this to correct alphabetical position in list.Google Scholar
  53. Frisk T, Ronnholm D, van der Wijngaart W and Stemme G (2006) A micromachined interface for airborne sample-to-liquid transfer and its application in a biosensor system. Lab. Chip 6:1504–1509Google Scholar
  54. Fung YS and Wong YY (2001) Self-assembled monolayers as the coating in a quartz piezoelectric crystal immunosensor to detect Salmonella in aqueous solution. Anal. Chem. 73: 5302–5309Google Scholar
  55. Furch M, Ueberfeld J, Hartmann A, Bock D and Seeger S (1996) Ultrathin oligonucleotide layers for fluorescence based DNA-sensors. Proc. SPIE Int. Soc. Opt. Eng. 2928:220–226Google Scholar
  56. Gaines GLJ (1966) Insoluble Monolayers at Liquid-gas Interfaces. Interscience, New YorkGoogle Scholar
  57. Gao H, Kislig E, Oranth N and Sigrist H (1994) Photolinker-polymer-mediated immobilization of monoclonal antibodies, F(ab’)2 and F(ab’) fragments. Biotechnol. Appl. Biochem. 20:251–263Google Scholar
  58. Gao Z, Tao G and Li G (1998) Research on detection of type C2 staphylococcus enterotoxin in food with piezoelectric immunosensors. Wei Sheng Yan Jiu 27:122–124Google Scholar
  59. Garnier J, Osguthorpe DJ and Robson B (1978) Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120:97–120Google Scholar
  60. Gawrisch K, Gaede HC, Luckett KM, Polozov IV and Yeliseev A (2005) Solid-supported membranes inside porous substrates and their use in biosensors, WIPO Patent WO/2005/069004 000069&LANGUAGE=EN (accessed April 11, 2007)Google Scholar
  61. Ghafouri S and Thompson M (1999) Interfacial properties of biotin conjugate avidin complexes studied by acoustic wave sensor. Langmuir 15:564–572Google Scholar
  62. Gizeli E (2002) Biomolecular Sensors. Taylor & Francis, Inc., New York, pp 176–206Google Scholar
  63. Goldman ER, Pazirandeh MP, Charles PT, Balighian E.D and Anderson GP (2002) Selection of phage displayed peptides for the detection of 2,4,6-trinitrotoluene in seawater. Anal. Chim. Acta 457:13–19Google Scholar
  64. Goldman ER, Pazirandeh MP, Mauro JM, King KD, Frey JC and Anderson GP (2000) Phage-displayed peptides as biosensor reagents. J. Mol. Recognit. 13:382–387Google Scholar
  65. Goodchild S, Love T, Hopkins N and Mayers C (2006) Engineering antibodies for biosensor technologies. Adv. Appl. Microbiol. 58:185–226Google Scholar
  66. Grate JW and Frye GC (1996) Acoustic wave sensors. Sens. Update 2:37–83Google Scholar
  67. Griffith J, Manning M and Dunn K (1981) Filamentous bacteriophage contract into hollow spherical particles upon exposure to a chloroform-water interface. Cell 23:747–753Google Scholar
  68. Harteveld JLN, Nieuwenhuizen MS and Wils ERJ (1997) Detection of staphylococcal enterotoxin B employing a piezoelectric crystal immunosensor. Biosens. Bioelectron. 12:661–667Google Scholar
  69. Hayden O, Bindeus R and Dickert FL (2003) Combining atomic force microscope and quartz crystal microbalance studies for cell detection. Meas. Sci. Technol. 14: 1876–1881Google Scholar
  70. Hayden O and Dickert FL (2001) Selective microorganism detection with cell surface imprinted polymers. Adv. Mater. 13:1480–1483Google Scholar
  71. He F, Geng Q, Zhu W, Nie L, Yao S and Meifeng C (1994) Rapid detection of Escherichia coli using a separated electrode piezoelectric crystal sensor. Anal. Chim. Acta 289:313–319Google Scholar
  72. He F and Zhang L (2002) Rapid diagnosis of M. tuberculosis using a piezoelectric immunosensor. Anal. Sci. 18:397–401MathSciNetGoogle Scholar
  73. He F, Zhang X, Zhou J and Liu Z (2006) A new MSPQC system for rapid detection of pathogens in clinical samples. J. Microbiol. Methods 66:56–62Google Scholar
  74. He F, Zhao J, Zhang L and Su X (2003) A rapid method for determining Mycobacterium tuberculosis based on a bulk acoustic wave impedance biosensor. Talanta 59:935–941Google Scholar
  75. He F and Zhou J (2007) A new antimicrobial susceptibility testing method of Escherichia coli against ampicillin by MSPQC. J. Microbiol. Methods 68:563–567MathSciNetGoogle Scholar
  76. Herriott RM and Barlow JL (1957) The protein coats or “ghosts” of Escherichia coli phage T2. Preparation, assay, and some chemical properties. J. Gen. Physiol. 40:809–825Google Scholar
  77. Horisberger M (1984) Electron-opaque markers: a review. In: Polak JM and Varndell IM (eds) Immunolabelling for Electron Microscopy. Elsevier, AmsterdamGoogle Scholar
  78. Horisberger M (1992) Colloidal gold and its application in cell biology. Int. Rev. Cytol. 136:227–87Google Scholar
  79. Houbiers MC and Hemminga MA (2004) Protein-lipid interactions of bacteriophage M13 gene 9 minor coat protein. Mol. Membr. Biol. 21:351–359Google Scholar
  80. Houbiers MC, Wolfs CJAM, Spruijt RB, Bollen YJM, Hemminga MA and Goormaghtigh E (2001) Conformation and orientation of the gene 9 minor coat protein of bacteriophage M13 in phospholipid bilayers. Biochim. Biophys. Acta Biomembr. 1511:224–235Google Scholar
  81. Howe E and Harding G (2000) A comparison of protocols for the optimisation of detection of bacteria using a surface acoustic wave (SAW) biosensor. Biosens. Bioelectron. 15:641–649Google Scholar
  82. Im W and Brooks CL (2004) De novo folding of membrane proteins: an exploration of the structure and NMR properties of the fd coat protein. J. Mol. Biol. 337:513–519Google Scholar
  83. Ishimori Y, Karube I and Suzuki IS (1981) Determination of microbial populations with piezoelectric membranes. Appl. Environ. Microbiol. 42:632–637Google Scholar
  84. Ivnitski D, Abdel-Hamid I, Atanasov P and Wilkins E (1999) Biosensors for detection of pathogenic bacteria. Biosens. Bioelectron. 14:599–624Google Scholar
  85. Janshoff A, Galla HJ and Steinem C (2000) Piezoelectric mass-sensing devices as biosensors-an alternative to optical biosensors? Angew. Chem. Int. Ed. Engl. 39:4004–4032Google Scholar
  86. Janshoff A and Steinem C (2001) Quartz crystal microbalance for bioanalytical applications. Sens. Update 9:313–354Google Scholar
  87. Janshoff A, Steinem C and Wegener J (2004) Noninvasive Electrical Sensor Devices to Monitor Living Cells Online. Ultrathin Electrochemical Chemo- and Biosensors. Springer, New YorkGoogle Scholar
  88. Jiang Y-B, Liu N, Gerung H, Cecchi JL and Brinker CJ (2006) Nanometer-thick conformal pore sealing of self-assembled mesoporous silica by plasma-assisted atomic layer deposition. J. Am. Chem. Soc. 128:11018–11019Google Scholar
  89. Kaspar M, Stadler H, Weiss T and Ziegler C (2000) Thickness shear mode resonators (“mass sensitive devices”) in bioanalysis. Fresenius J. Anal. Chem. 366:602–610Google Scholar
  90. Kim G-H, Rand AG and Letcher SV (2003) Impedance characterization of a piezoelectric immunosensor part II: Salmonella typhimurium detection using magnetic enhancement. Biosens. Bioelectron. 18:91–99Google Scholar
  91. Kim N and Park I-S (2003) Application of a flow-type antibody sensor to the detection of Escherichia coli in various foods. Biosens. Bioelectron. 18:1101–1107Google Scholar
  92. Kim N, Park I-S and Kim D-K (2004) Characteristics of a label-free piezoelectric immunosensor detecting Pseudomonas aeruginosa. Sens. Actuators B 100:432–438Google Scholar
  93. King WH (1964) Piezoelectric sorption detector. Anal. Chem. 36:1735–1739Google Scholar
  94. Kleinschmidt AK, Lang D, Jacherts D and Zahn RK (1962) Preparation and length measurements of the total deoxyribonucleic acid (DNA) content of T2 bacteriophages. Biochim. Biophys. Acta 61:875–864Google Scholar
  95. Konig B and Gratzel M (1993) Detection of viruses and bacteria with piezoelectric immunosensors. Anal. Lett. 26:1567–1585Google Scholar
  96. Konig B and Gratzel M (1995) A piezoelectric immunosensor for hepatitis viruses. Anal. Chim. Acta 309:19–25Google Scholar
  97. Kouzmitcheva G (2005) Personal communication.Google Scholar
  98. Krause R (1993) Process control for Ni/Au plating with QCM technology. Circuitree 6:10–12Google Scholar
  99. Kreth J, Hagerman E, Tam K, Merritt J, Wong D, Wu B, Myung N, Shi W and Qi F (2004) Quantitative analyses of Streptococcus mutans biofilms with quartz crystal microbalance, microjet impingement and confocal microscopy. Biofilms 1:277–284Google Scholar
  100. Kurosawa S, Park J, Aizawa H, Wakida S, Tao H and Ishihara K (2006) Quartz crystal microbalance immunosensors for environmental monitoring. Biosens. Bioelectron. 22:473–481Google Scholar
  101. Lakshmanan RS, Hu J, Guntupalli R, Wan J, Huang S, Yang H, Petrenko VA, Barbaree JM and Chin BA (2006) Detection of Salmonella typhimurium using phage based magnetostrictive sensor. Proc. SPIE Int. Soc. Opt. Eng. 6218:62180ZGoogle Scholar
  102. Lazcka O, Campo FJD and Munoz FX (2007) Pathogen detection: A perspective of traditional methods and biosensors. Biosens. Bioelectron. 22:1205–1217Google Scholar
  103. Le D, He F-J, Jiang TJ, Nie L and Yao S (1995) A goat-anti-human IgG modified piezoimmunosensor for Staphylococcus aureus detection. J. Microbiol. Methods 23:229–234Google Scholar
  104. Leonard P, Hearty S, Brennan J, Dunne L, Quinn J, Chakraborty T and O’Kennedy R (2003) Advances in biosensors for detection of pathogens in food and water. Enzyme Microb. Technol. 32:3–13Google Scholar
  105. Lin H-C and Tsai W-C (2003) Piezoelectric crystal immunosensor for the detection of staphylococcal enterotoxin B. Biosens. Bioelectron. 18:1479–1483Google Scholar
  106. Love AEH (1911) Some problems of geodynamics. Cambridge: CambridgeGoogle Scholar
  107. Lucklum R (2005) Non-gravimetric contributions to QCR sensor response. Analyst 130:1465–1473Google Scholar
  108. Mao X, Yang L, Su X-L and Li Y (2006) A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7. Biosens. Bioelectron. 21:1178–1185Google Scholar
  109. Martin SJ, Granstaff VE and Frye GC (1991) Characterization of quartz crystal microbalance with simultaneous mass and liquid loading. Anal. Chem. 63:2272–2281Google Scholar
  110. Marxer C, Coen M, Greber T, Greber U and Schlapbach L (2003) Cell spreading on quartz crystal microbalance elicits positive frequency shifts indicative of viscosity changes. Anal. Bioanal. Chem. 377:578–586Google Scholar
  111. Mead P, Slutsker L, Dietz V, McCaig L, Bresee J, Shapiro C, Griffin P and Tauxe RV (1999) Food-related illness and death in the United States. Emerg. Infect. Dis. 5:607–625CrossRefGoogle Scholar
  112. Minunni M, Skladal P and Mascini M (1994) A piezoelectric quartz crystal biosensor as a direct affinity sensor. Anal. Lett. 27:1475–1487Google Scholar
  113. Mittler-Neher S, Spinke J, Liley M, Nelles G, Weisser M, Back R, Wenz G and Knoll W (1995) Spectroscopic and surface-analytical characterization of self-assembled layers on Au. Biosens. Bioelectron. 10:903–916Google Scholar
  114. Mo X-T, Zhou Y-P, Lei H and Deng L (2002) Microbalance-DNA probe method for the detection of specific bacteria in water. Enzyme Microb. Technol. 30:583–589Google Scholar
  115. Moll N, Pascal E, Dinh DH, Pillot J-P, Bennetau B, Rebiere D, Moynet D, Mas Y, Mossalayi D, Pistre J and Dejous C (2007) A Love wave immunosensor for whole E. coli bacteria detection using an innovative two-step immobilisation approach. Biosens. Bioelectron. 22:2145–2150Google Scholar
  116. Morgan DP (2000) A history of surface acoustic wave devices. Int. J. High Speed Electron. Syst. 10:553–602Google Scholar
  117. Muramatsu H, Kajiwara K, Tamiya E, Karube I (1986) Piezoelectric immunosensor for the detection of Candida albicans microbes. Anal. Chim. Acta 188:257–261Google Scholar
  118. Muratsugu M, Ohta F, Miya Y, Hosokawa T, Kurosawa S, Kamo N and Ikeda H (1993) Quartz crystal microbalance for the detection of microgram quantities of human serum albumin: relationship between the frequency change and the mass of protein adsorbed. Anal. Chem. 65:2933–2937Google Scholar
  119. Nakanishi K, Karube I, Hiroshi S, Uchida A and Ishida Y (1996) Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor. Anal. Chim. Acta 325:73–80Google Scholar
  120. Nanduri V (2005) Phage at the air-liquid interface for the fabrication of biosensors. Doctoral Dissertation. Auburn University, AlabamaGoogle Scholar
  121. Nanduri V, Sorokulova IB, Samoylov AM, Simonian AL, Petrenko VA, Vodyanoy V (2007) Phage as a molecular recognition element in biosensors immobilized by physical adsorption. Biosens. Bioelectron. 22:986–992Google Scholar
  122. Neidhardt FC (1987) Escherichia coli and Salmonella. American Society For Microbiology, Washington, D.C.Google Scholar
  123. Nishiyama Y, Tanaka S, Hillhouse HW, Nishiyama N, Egashira Y and Ueyama K (2006) Synthesis of ordered mesoporous zirconium phosphate films by spin coating and vapor treatments. Langmuir 22:9469–9472Google Scholar
  124. Niven D, Chambers J, Anderson T and White D (1993) Long-term, on-line monitoring of microbial biofilms using a quartz crystal microbalance. Anal. Chem. 65:65–69Google Scholar
  125. O’Sullivan CK and Guilbault GG (1999) Commercial quartz crystal microbalances—theory and applications. Biosens. Bioelectron. 14:663–670Google Scholar
  126. Olsen EV (2000) Functional durability of a quartz crystal microbalance sensor for the rapid detection of Salmonella in liquids from poultry packaging. Masters Thesis. Auburn University, Alabama (accessed April 10, 2007)Google Scholar
  127. Olsen EV (2005) Phage-coupled piezoelectric biodetector for Salmonella typhimurium. Doctoral Dissertation. Auburn University, Alabama (accessed April 10, 2007)Google Scholar
  128. Olsen EV, Pathirana ST, Samoylov AM, Barbaree JM, Chin BA, Neely WC and Vodyanoy V (2003) Specific and selective biosensor for Salmonella and its detection in the environment. J. Microbiol. Methods 53:273–285Google Scholar
  129. Olsen EV, Sorokulova IB, Petrenko VA, Chen IH, Barbaree JM and Vodyanoy VJ (2006) Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium. Biosens. Bioelectron. 21:1434–1442Google Scholar
  130. Olsen EV, Sykora JC, Sorokulova IB, Chen I-H, Neely WC, Barbaree JM, Petrenko VA and Vodyanoy VJ (2007) Phage fusion proteins as bioselective receptors for piezoelectric sensors. Electrochem. Soc. Trans. 2:9–25Google Scholar
  131. Otto K, Elwing H and Hermansson M (1999) Effect of ionic strength on initial interactions of Escherichia coli with surfaces, studied on-line by a novel quartz crystal microbalance technique. J. Bacteriol. 181:5210–5218Google Scholar
  132. Ozen A, Montgomery K, Jegier P, Patterson S, Daumer KA, Ripp SA, Garland JL and Sayler GS (2004) Development of bacteriophage-based bioluminescent bioreporters for monitoring of microbial pathogens. Proc. SPIE Int. Soc. Opt. Eng. 5270:58–68Google Scholar
  133. Park I-S and Kim N (1998) Thiolated Salmonella antibody immobilization onto the gold surface of piezoelectric quartz crystal. Biosens. Bioelectron. 13:1091–1097Google Scholar
  134. Park I-S, Kim W-Y and Kim N (2000) Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp. Biosens. Bioelectron. 15:167–172Google Scholar
  135. Pathirana S, Myers LJ, Vodyanoy V and Neely WC (1996) Assembly of cadmium stearate and valinomycin molecules assists complexing of K+ in mixed Langmuir-Blodgett films. Supramol. Sci. 2:149–154Google Scholar
  136. Pathirana S, Neely WC, Myers LJ and Vodyanoy V (1992) Interaction of valinomycin and stearic acid in monolayers. Langmuir 8:1984–1987Google Scholar
  137. Pathirana S, Neely WC and Vodyanoy V (1998) Condensing and expanding the effects of the odorants (+)- and (-)-carvone on phospholipid monolayers. Langmuir 14:679–682Google Scholar
  138. Pathirana ST (1993) Interaction of valinomycin and stearic acid in monolayers at the air/water interface. Doctoral Dissertation. Auburn University, AlabamaGoogle Scholar
  139. Pathirana ST, Barbaree J, Chin BA, Hartell MG, Neely WC and Vodyanoy V (2000) Rapid and sensitive biosensor for Salmonella. Biosens. Bioelectron. 15:135–141Google Scholar
  140. Pattus F, Desnuelle P and Verger R (1978) Spreading of liposomes at the air/water interface. Biochim. Biophys. Acta 507:62–70Google Scholar
  141. Pattus F and Rothen C (1981) Lipid-protein interactions in monolayers at the air-water interface. In: Azzi A, Brodbeck U, Zahler P (eds) Membrane Proteins. Springer-Verlag, Berlin pp 229–240Google Scholar
  142. Pattus F, Rothen C, Streit M and Zahler P (1981) Further studies on the spreading of biomembranes at the air/water interface. Structure, composition, enzymatic activities of human erythrocyte and sarcoplasmic reticulum membrane films. Biochim. Biophys. Acta Biomembr. 647:29–39Google Scholar
  143. Pavey KD (2002) Quartz crystal analytical sensors: the future of label-free, real-time diagnostics? Expert Rev. Mol. Diag. 2:173–186Google Scholar
  144. Pavey KD, Ali Z, Olliff CJ and Paul F 1999. Application of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutination. J. Pharm. Biomed. Anal. 20:241–245Google Scholar
  145. Pavey KD, Barnes L, Hanlon G, Olliff C, Ali Z and Paul F (2001) A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology. Lett. Appl. Microbiol. 33:344–348Google Scholar
  146. Pereira de Jesus D, Naves C and Lucia do Lago C (2002) Determination of boron by using a quartz crystal resonator coated with N-Methyl-D-glucamine-modified poly(epichlorohydrin). Anal. Chem. 74:3274–3280Google Scholar
  147. Petrenko VA (2004) Personal communication.Google Scholar
  148. Petrenko VA and Smith GP (2000) Phages from landscape libraries as substitute antibodies. Protein Eng. 13:589–592Google Scholar
  149. Petrenko VA, Sorokulova IB, Chin BA, Barbaree JM, Vodyanoy VJ, Chen IH and Samoylov AM (2005) Biospecific peptide probes against Salmonella isolated from phage display libraries, and their diagnostic and drug delivery uses. US Patent Application 20050137136, filed Apr 29, 2004Google Scholar
  150. Petrenko VA and Vodyanoy VJ (2003) Phage display for detection of biological threat agents. J. Microbiol. Methods 53:253–262Google Scholar
  151. Petrenko VA, Vodyanoy VJ and Sykora JC (2007) Methods of forming monolayers of phage-derived products and uses thereof. US Patent # 7, 267, 993Google Scholar
  152. Petty MC (1991) Application of multilayer films to molecular sensors: some examples of bioengineering at the molecular level. J. Biomed. Eng. 13:209–214Google Scholar
  153. PM-740 Series Operation and Service Manual. (1996) Maxtek, Inc., Sante Fe Springs, CaliforniaGoogle Scholar
  154. Pohanka M and Skládal P (2005) Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution. Anal. Lett. 38:411–422Google Scholar
  155. Prusak-Sochaczewski E and Luong JHT (1990) Development of a piezoelectric immunosensor for the detection of Salmonella typhimurium. Enzyme and Microb. Technol. 12:173–177Google Scholar
  156. Pyun JC, Beutel H, Meyer JU and Ruf HH (1998) Development of a biosensor for E. coli based on a flexural plate wave (FPW) transducer. Biosens. Bioelectron 13:839–845Google Scholar
  157. Qu X, Bao L, Su X and Wei W (1998) Rapid detection of Escherichia coliform with a bulk acoustic wave sensor based on the gelation of Tachypleus amebocyte lysate. Talanta 47:285–290Google Scholar
  158. Ramirez E, Mas JM, Carbonell X, Aviles FX and Villaverde A (1999) Detection of molecular interactions by using a new peptide-displaying bacteriophage. Biosensor. Biochem. Biophys. Res. Commun. 262:801–805Google Scholar
  159. Ramsden JJ (1997a) Dynamics of protein adsorption at the solid/liquid interface. Recent Res. Dev. Phys. Chem. 1:133–142Google Scholar
  160. Ramsden JJ (1997b) Protein adsorption at the solid/liquid interface. Conference on Colloid Chemistry: In Memoriam Aladar Buzagh, Proceedings, 7th, Eger, Hung., Sept. 23–26, 1996, 148–151Google Scholar
  161. Ramsden JJ (1998) Biomimetic protein immobilization using lipid bilayers. Biosens. Bioelectronics 13:593–598Google Scholar
  162. Ramsden JJ (1999) On protein-lipid membrane interactions. Colloids Surfaces B Biointerfaces 14:77–81Google Scholar
  163. Ramsden JJ (2001) Multiple interactions in protein-membrane binding. NATO Science Series, 335:244–269. IOS Press, AmsterdamGoogle Scholar
  164. Rayleigh L (1885) On waves propagating along the plane surface of an elastic solid. Proc. London Math. Soc. 17:4–11Google Scholar
  165. Reipa V, Almeida J and Cole KD (2006) Long-term monitoring of biofilm growth and disinfection using a quartz crystal microbalance and reflectance measurements. J. Microbiol. Methods 66:449–459Google Scholar
  166. Rickert J, Gopel W, Hayward GL, Cavic BA and Thompson M (1999) Biosensors based on acoustic wave devices. Sens. Update 5:105–139Google Scholar
  167. Roberts LM and Dunker AK (1993) Structural changes accompanying chloroform-induced contraction of the filamentous phage fd. Biochemistry 32:10479–10488Google Scholar
  168. Samoylov AM, Samoylova TI, Hartell MG, Pathirana ST, Smith BF and Vodyanoy V (2002a) Recognition of cell-specific binding of phage display derived peptides using an acoustic wave sensor. Biomol. Eng. 18:269–272Google Scholar
  169. Samoylov AM, Samoylova TI, Pathirana ST, Globa LP and Vodyanoy VJ (2002b) Peptide biosensor for recognition of cross-species cell surface markers. J. Mol. Recognit. 15:197–203Google Scholar
  170. Sauerbrey GZZ (1959) Use of quartz vibrator for weighing thin films on a microbalance. Z. Phys. 155:206–212Google Scholar
  171. Sayler GS, Ripp SA, Applegate BM (2003) Bioluminescent biosensor device. US Patent Application 20030027241, filed July 20, 2001Google Scholar
  172. Scherz P (2000) Oscillators and Timers. McGraw-Hill, Inc., New YorkGoogle Scholar
  173. Selz KA, Samoylova TI, Samoylov AM, Vodyanoy VJ and Mandell AJ (2006) Designing allosteric peptide ligands targeting a globular protein. Biopolymers 85:38–59Google Scholar
  174. Si S, Lia X, Fungb Y and Zhub D (2001) Rapid detection of Salmonella enteritidis by piezoelectric immunosensor. Microchem. J. 68:21–27Google Scholar
  175. Si S, Ren F, Cheng W and Yao S (1997) Preparation of a piezoelectric immunosensor for the detection of Salmonella paratyphi A by immobilization of antibodies on electropolymerized films. Fresenius J. Anal. Chem. 357:1101–1105Google Scholar
  176. Skládal P (2003) Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions. J. Braz. Chem. Soc. 14:491–502Google Scholar
  177. Sobotka H and Trurnit HJ (1961) Need title of their article here. In: Alexander P, Block RJ (eds) Analytical Methods of Protein Chemistry. Pergamon Press, Oxford, UK, pp 212–243Google Scholar
  178. Song M-J, Yun D-H, Jin J-H, Min N-K and Hong S-I (2006) Comparison of effective working electrode areas on planar and porous silicon substrates for cholesterol biosensor. Jpn. J. Appl. Phys. 45:7197–7202Google Scholar
  179. Sorial J and Lec R (2004) A piezoelectric interfacial phenomena biosensor. Masters Thesis. Drexel University, Pennsylvania (accessed April 10, 2007)Google Scholar
  180. Sorokulova IB, Olsen EV, Chen IH, Fiebor B, Barbaree JM, Vodyanoy VJ, Chin BA and Petrenko VA (2005) Landscape phage probes for Salmonella typhimurium. J. Microbiol. Methods 63:55–72Google Scholar
  181. Spangler BD, Wilkinson EA, Murphy JT and Tyler BJ (2001) Comparison of the Spreeta®surface plasmon resonance sensor and a quartz crystal microbalance for detection of Escherichia coli heat-labile enterotoxin. Anal. Chim. Acta 444:149–161Google Scholar
  182. Stadler H, Mondon M and Ziegler C (2003) Protein adsorption on surfaces: dynamic contact angle (DCA) and quartz-crystal microbalance (QCM) measurements. Anal. Bioanal. Chem 375:53–61Google Scholar
  183. Storri S, Santoni T and Mascini M (1998) A piezoelectric biosensor for DNA hybridisation detection. Anal. Lett. 31:1795–1808Google Scholar
  184. Su C-C, Wu T-Z, Chen L-K, Yang H-H and Tai D-F (2003) Development of immunochips for the detection of dengue viral antigens. Anal. Chim. Acta 479:117–123Google Scholar
  185. Su X-L and Li SFY (2001) Serological determination of Helicobacter pylori infection using sandwiched and enzymatically amplified piezoelectric biosensor. Anal. Chim. Acta 429:27–36Google Scholar
  186. Su X-L and Li Y (2004) A self-assembled monolayer-based piezoelectric immunosensor for rapid detection of Escherichia coli O157:H7. Biosens. Bioelectron. 19:563–574Google Scholar
  187. Su X-L and Li Y (2005) A QCM immunosensor for Salmonella detection with simultaneous measurements of resonant frequency and motional resistance. Biosens. Bioelectron. 21:840–848MathSciNetGoogle Scholar
  188. Su X, Low S, Kwang J, Chew VHT and Li SFY (2001) Piezoelectric quartz crystal based veterinary diagnosis for Salmonella enteritidis infection in chicken and egg. Sens. Actuators B 75:29–35Google Scholar
  189. Sukhorukov GB, Montrel MM, Petrov AI, Shabarchina LI and Sukhorukov BI (1996) Multilayer films containing immobilized nucleic acids. Their structure and possibilities in biosensor applications. Biosens. Bioelectron. 11:913–922Google Scholar
  190. Sykora JC (2003) Monolayers of biomolecules for recognition and transduction in biosensors. Doctoral Dissertation. Auburn University, Al. (accessed April 10, 2007)Google Scholar
  191. Sykora JC, Neely WC and Vodyanoy V (2004) Solvent effects on amphotericin B monolayers. J. Colloid Interface Sci. 269:499–502Google Scholar
  192. Tabacco MB, Qian X and Russo J (2004) Fluorescent virus probes for identification of bacteria. US Patent Application 20040191859, filed Mar 24, 2003Google Scholar
  193. Tai D, Lin C, Wu T, Huang J and Shu P (2006) Artificial receptors in serologic tests for the early diagnosis of dengue virus infection. Clin. Chem. 58:1486–1491Google Scholar
  194. Tan H, Deng L, Nie L and Yao S (1997) Detection and analysis of the growth characteristics of Proteus vulgaris with a bulk acoustic wave ammonia sensor. Analyst 122:179–184Google Scholar
  195. Thompson M, Arthur C and Dhaliwal G (1986) Liquid-phase piezoelectric and acoustic transmission studies of interfacial immunochemistry. Anal. Chem. 58:1206–1209Google Scholar
  196. Thompson M, Kiplingt A, Duncan-Hewitt W, Rajakovic L and Cavic-Vlasak B (1991) Thickness-shear-mode acoustic wave sensors in the liquid phase: a review. Analyst 116:881–890Google Scholar
  197. Thust M, Schoning MJ, Schroth P, Malkoc U, Dicker CI, Steffen A, Kordos P and Luth H (1999) Enzyme immobilization on planar and porous silicon substrates for biosensor applications. J. Mol. Catal. B: Enzym. 7:77–83Google Scholar
  198. Tiefenauer LX, Kossek S, Padeste C and Thiebaud P (1997) Towards amperometric immunosensor devices. Biosens. Bioelectron. 12:213–223Google Scholar
  199. Trurnit HJ (1960) The spreading of protein monolayers. J. Colloid Sci. 15:1–13Google Scholar
  200. Umezawa Y (1996) CRC handbook of ion-selective electrodes: selectivity coefficients. CRC Press, Boca Raton, FloridaGoogle Scholar
  201. Uttenthaler E, Kolinger C and Drost S (1998) Quartz crystal biosensor for detection of the African Swine Fever disease. Anal. Chim. Acta 362:91–100Google Scholar
  202. Uttenthaler E, Schraml M, Mandel J and Drost S (2001) Ultrasensitive quartz crystal microbalance sensors for detection of M13-Phages in liquids. Biosens. Bioelectronics 16:735–743Google Scholar
  203. Uzawa H, Kamiya S, Minoura N, Dohi H, Nishida Y, Taguchi K, Yokoyama S, Mori H, Shimizu T and Kobayashi K (2002) A quartz crystal microbalance method for rapid detection and differentiation of shiga toxins by applying a monoalkyl globobioside as the toxin ligand. Biomacromolecules 3:411–414Google Scholar
  204. Vaughan RD, O’Sullivan CK and Guilbault GG (2001) Development of a quartz crystal microbalance (QCM) immunosensor for the detection of Listeria monocytogenes. Enzyme Microb. Technol. 29:635–638Google Scholar
  205. Vaughan TJ, Williams AJ, Pritchard K, Osbourn JK, Pope AR, Earnshaw JC, McCafferty J, Hodits RA, Wilton J and Johnson KS (1996) Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nature Biotechnol. 14:309–314Google Scholar
  206. Victorov IA (1967) Rayleigh and Lamb Waves—physical theory and applications. Plenum Press, New YorkGoogle Scholar
  207. Vig J and Ballato A (1998) Comments about the effects of non-uniform mass loading on a quartz crystal microbalance. IEEE Trans. Ultrason. Ferroelectrics Freq. Contr. 45:1123–1124Google Scholar
  208. Vodyanoy V (1994) Functional reconstitution of mammalian olfactory receptor. Report. ARO-27364.10-LSGoogle Scholar
  209. Voinova MV, Jonson M and Kasemo B (2002) “Missing mass” effect in biosensor’s QCM applications. Biosens. Bioelectron. 17:835–841Google Scholar
  210. Volker M and Siegmund HU (1997) Forster energy transfer in ultrathin polymer layers as a basis for biosensors. EXS 80:175–191Google Scholar
  211. Wang J, Fu W, Liu M, Wang Y, Xue Q, Huang J and Zhu Q (2002) Multichannel piezoelectric genesensor for the detection of human papilloma virus. Chinese Med. J. (Engl) 115:439–442Google Scholar
  212. White RM and Voltmer FW (1965) Direct piezoelectric coupling to surface elastic waves. Appl. Phys. Lett. 7:314–316Google Scholar
  213. Wong YY, Ng SP, Ng MH, Si SH, Yao SZ and Fung YS (2002) Immunosensor for the differentiation and detection of Salmonella species based on a quartz crystal microbalance. Biosens. Bioelectron. 17:676–684Google Scholar
  214. Wu T-Z, Su C-C, Chen L-K, Yang H-H, Tai D-F and Peng K-C (2005) Piezoelectric immunochip for the detection of dengue fever in viremia phase. Biosens. Bioelectron. 21:689–695Google Scholar
  215. Wu Z-Y, Shen G-L, Li Z-Q, Wang S-P and Yu R-Q (1999) A direct immunoassay for Schistosoma japonium antibody (SjAb) in serum by piezoelectric body acoustic wave sensor. Anal. Chim. Acta 398:57–63Google Scholar
  216. Wu Z, Wu J, Wang S, Shen G and Yu R (2006) An amplified mass piezoelectric immunosensor for Schistosoma japonicum. Biosens. Bioelectron. 22:207–212Google Scholar
  217. Xomeritakis G, Liu NG, Chen Z, Jiang YB, Koehn R, Johnson PE, Tsai CY, Shah PB, Khalil S, Singh S and Brinker CJ (2007) Anodic alumina supported dual-layer microporous silica membranes. J. Membr. Sci. 287:157–161Google Scholar
  218. Yakhno T, Sanin A, Pelyushenko A, Kazakov V, Shaposhnikova O, Chernov A, Yakhno V, Vacca C, Falcione F and Johnson B (2007) Uncoated quartz resonator as a universal biosensor. Biosens. Bioelectron. 22:2127–2131Google Scholar
  219. Yao S, Tan H, Zhang H, Su X and Wei W (1998) Bulk acoustic wave bacterial growth sensor applied to analysis of antimicrobial properties of tea. Biotechnol. Prog. 14:639–644Google Scholar
  220. Ye J, Letcher S and Rand AG (1997) Piezoelectric biosensor for the detection of Salmonella typhimurium. J. Food Sci. 62:1067–1071, 1086Google Scholar
  221. Yilma S, Cannon-Sykora J, Samoylov A, Lo T, Liu N, Brinker CJ, Neely WC and Vodyanoy V (2007a) Large-conductance cholesterol-amphotericin B channels in reconstituted lipid bilayers. Biosens. Bioelectron. 22:1359–1367Google Scholar
  222. Yilma S, Liu N, Samoylov A, Lo T, Brinker CJ and Vodyanoy V (2007b) Amphotericin B channels in phospholipid membrane-coated nanoporous silicon surfaces: implications for photovoltaic driving of ions across membranes. Biosens. Bioelectron. 22:1605–1611Google Scholar
  223. Ying-Sing F, Shi-Hui S and De-Rong Z (2000) Piezoelectric crystal for sensing bacteria by immobilizing antibodies on divinylsulphone activated poly-m-aminophenol film. Talanta 51:151–158Google Scholar
  224. Yun D-H, Song M-J, Hong S-I, Kang M-S and Min N-K (2005) Highly sensitive and renewable amperometric urea sensor based on self-assembled monolayer using porous silicon substrate. J. Kor. Phys. Soc. 47:S445–S449Google Scholar
  225. Zhang J, Xie Y, Dai X, Wei W (2001) Monitoring of Lactobacillus fermentation process by using ion chromatography with a series piezoelectric quartz crystal detector. J. Microbiol. Methods 44:105–111zbMATHGoogle Scholar
  226. Zhang S, Wei W, Zhang J, Mao Y and Liu S (2002) Effect of static magnetic field on growth of Escherichia coli and relative response model of series piezoelectric quartz crystal. Analyst 127:373–377Google Scholar
  227. Zhao J, Zhu W and He F (2005) Rapidly determining E. coli and P. aeruginosa by an eight channels bulk acoustic wave impedance physical biosensor. Sens. Actuators B 107: 271–276Google Scholar
  228. Zhou X, Liu L, Hu M, Wang L and Hu J (2002) Detection of hepatitis B virus by piezoelectric biosensor. J. Pharmaceut. Biomed. Anal. 27:341–345Google Scholar
  229. Zuo B, Li S, Guo Z, Zhang J and Chen C (2004) Piezoelectric immunosensor for SARS-associated coronavirus in sputum. Anal. Chem. 76:3536–3540Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Eric Olsen
    • 1
  • Arnold Vainrub
    • 2
  • Vitaly Vodyanoy
    • 2
  1. 1.Clinical Investigation FacilityDavid Grant USAF Medical CenterUSA
  2. 2.Department of AnatomyPhysiology and Pharmacology Auburn UniversityAuburnUSA

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