αvβ5 Integrin Receptors at the Apical Surface of the RPE: One Receptor, Two Functions

  • Emeline F. Nandrot
  • Yongen Chang
  • Silvia C. Finnemann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 613)

Photoreceptors and retinal pigment epithelial (RPE) cells, two adjacent cells types of the outer retina, interact with each other functionally in numerous ways. Maintenance of permanent retinal adhesion and cyclic phagocytosis of shed photoreceptor outer segment fragments (POS) by RPE cells are two forms of these interactions that are crucial for vision. RPE cells form a polarized monolayer and extend apical microvilli that ensheath photoreceptor outer segments. Outer segments consist of stacked membranous disks containing the phototransduction machinery and are permanently renewed. To maintain constant outer segment length photoreceptors eliminate their most aged tips by daily shedding (Young, 1967), which precedes a burst of phagocytosis by the RPE that efficiently clears POS from the subretinal space and recycles many of their components (Young and Bok, 1969). POS shedding and subsequent phagocytosis by RPE cells are critical for photoreceptor cell function and long term survival.

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References

  1. Cook, B., Lewis, G. P., Fisher S. K., and Adler, R., 1995, Apoptotic photoreceptor degeneration in experimental retinal detachment, Invest. Ophthalmol. Vis. Sci. 36:990–996.PubMedGoogle Scholar
  2. Edwards, R. B., and Szamier, R. B., 1977, Defective phagocytosis of isolated rod outer segments by RCS rat retinal pigment epithelium in culture, Science. 197:1001–1003.PubMedCrossRefGoogle Scholar
  3. Endo, E. G., Yao, X. Y., and Marmor, M. F., 1988, Pigment adherence as a measure of retinal adhesion: dependence on temperature, Invest. Ophthalmol. Vis. Sci. 29:1390–1396.PubMedGoogle Scholar
  4. Feeney, L., 1978, Lipofuscin and melanin of human retinal pigment epithelium. Fluorescence, enzyme cytochemical, and ultrastructural studies, Invest. Ophthalmol. Vis. Sci. 17:583–600.PubMedGoogle Scholar
  5. Finnemann, S. C., Bonilha, V. L., Marmorstein, A. D., and Rodriguez-Boulan, E., 1997, Phagocytosis of rod outer segments by retinal pigment epithelial cells requires αvβ5 integrin for binding but not for internalization, Proc. Natl. Acad. Sci. U. S. A. 94:12932–12937.PubMedCrossRefGoogle Scholar
  6. Finnemann, S. C., Leung, L. W., and Rodriguez-Boulan, E., 2002, The lipofuscin component A2E selectively inhibits phagolysosomal degradation of photoreceptor phospholipid by the retinal pigment epithelium, Proc. Natl. Acad. Sci. U. S. A. 99:3842–3847.PubMedCrossRefGoogle Scholar
  7. Finnemann, S. C., 2003, Focal adhesion kinase signaling promotes phagocytosis of integrin-bound photoreceptors, EMBO J. 22:4143–4154.PubMedCrossRefGoogle Scholar
  8. Finnemann, S. C., and Chang, Y., Photoreceptor-RPE interactions: physiology and molecular mechanisms. (In press) In: Visual Transduction and Non Visual Light Perception, J. Tombran-Tink and C.J. Barnstable, ed., Humana Press, Totowa, New Jersey.Google Scholar
  9. Goldman, A. I., Teirstein, P. S., and O’Brien, P. J., 1980, The role of ambient lighting in circadian disc shedding in the rod outer segment of the rat retina, Invest. Ophthalmol. Vis. Sci. 19:1257–1267.PubMedGoogle Scholar
  10. Hageman, G. S., Marmor, M. F., Yao, X. Y., and Johnson, L. V., 1995, The interphotoreceptor matrix mediates primate retinal adhesion, Arch. Ophthalmol. 113:655–660.PubMedGoogle Scholar
  11. Hollyfield, J. G., Varner, H. H., Rayborn, M. E., and Osterfeld, A. M., 1989, Retinal attachment to the pigment epithelium, Retina. 9:59–68.PubMedCrossRefGoogle Scholar
  12. Hollyfield, J. G., 1999, Hyaluronan and the functional organization of the interphotoreceptor matrix, Invest. Ophthalmol. Vis. Sci. 40:2767–2769.PubMedGoogle Scholar
  13. Huang, X., Griffiths, M., Wu, J., Farese, R. V., Jr., and Sheppard, D., 2000, Normal development, wound healing, and adenovirus susceptibility in β5-deficient mice, Mol. Cell. Biol. 20:755–759.PubMedCrossRefGoogle Scholar
  14. LaVail, M. M., 1976, Rod outer segment disk shedding in rat retina: relationship to cyclic lighting, Science. 194:1071–1074.PubMedCrossRefGoogle Scholar
  15. Mullen, R. J., and LaVail, M. M., 1976, Inherited retinal dystrophy: primary defect in pigment epithelium determined with experimental rat chimeras, Science. 192:799–801.PubMedCrossRefGoogle Scholar
  16. Nandrot, E. F., Kim, Y., Brodie, S. E., Huang, X., Sheppard, D., and Finnemann, S. C., 2004, Loss of synchronized retinal phagocytosis and age-related blindness in mice lacking αvβ5 integrin, J. Exp. Med. 200:1539–1545.PubMedCrossRefGoogle Scholar
  17. Nandrot, E. F., Anand, M., Sircar, M., and Finnemann, S. C., 2006, Novel role for αvβ5-integrin in retinal adhesion and its diurnal peak, Am. J. Physiol. Cell. Physiol. 290:C1256-C1262.PubMedCrossRefGoogle Scholar
  18. Schutt, F., Bergmann, M., Holz, F. G., Dithmar, S., Volcker, H. E., and Kopitz, J., 2006, Accumulation of A2-E in mitochondrial membranes of cultured RPE cells, Graefes. Arch. Clin. Exp. Ophthalmol. [epub ahead of print].Google Scholar
  19. Young, R.W., 1967, The renewal of photoreceptor cell outer segments, J. Cell Biol. 33:61–72.PubMedCrossRefGoogle Scholar
  20. Young, R. W., 1977, The daily rhythm of shedding and degradation of cone outer segment membranes in the lizard retina, J. Ultrastruct. Res. 61:172–185.PubMedCrossRefGoogle Scholar
  21. Young, R. W., and Bok, D., 1969, Participation of the retinal pigment epithelium in the rod outer segment renewal process, J. Cell Biol. 42:392–403.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Emeline F. Nandrot
    • 1
  • Yongen Chang
    • 2
  • Silvia C. Finnemann
    • 3
  1. 1.Margaret M. Dyson Vision Research Institute, Department of OphthalmologyWeill Medical College of Cornell UniversityNew YorkUSA
  2. 2.Margaret M. Dyson Vision Research Institute, Department of OphthalmologyWeill Medical College of Cornell UniversityNew YorkUSA
  3. 3.Margaret M. Dyson Vision Research Institute, Department of Ophthalmology; Department of Cell and Developmental Biology, Department of Physiology and BiophysicsWeill Medical College of Cornell UniversityNew YorkUSA

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