Milk Fat Globule Membrane Components–A Proteomic Approach
The milk fat globule membrane (MFGM) is the membrane surrounding lipid droplets during their secretion in the alveolar lumen of the lactating mammary gland. MFGM proteins represent only 1–4% of total milk protein content; nevertheless, the MFGM consists of a complex system of integral and peripheral proteins, enzymes, and lipids. Despite their low classical nutritional value, MFGMproteins have been reported to play an important role in various cellular processes and defense mechanisms in the newborn.
Using a proteomic approach, such as high-resolution, two-dimensional electrophoresis followed by direct protein identification by mass spectrometry, it has been possible to comprehensively characterize the subcellular organization of MFGM.
This chapter covers the description of MFGM proteomics from the first studies about 10 years ago through the most recent papers. Most of the investigations deal with MFGMs from human and cow milk.
KeywordsLipid Droplet Xanthine Oxidase Proteomic Approach Peptide Mass Fingerprinting Lipid Globule
Unable to display preview. Download preview PDF.
- Cavaletto, M., Giuffrida, M. G., Fortunato, D., Gardano, L., Dellavalle, G., Napolitano, L., Giunta, C., Bertino, E., Fabris, C., & Conti, A. (2002). A proteomic approach to evaluate the butyrophilin gene family expression in human milk fat globule membrane. Proteomics, 2, 850–856.CrossRefGoogle Scholar
- O’Farrel, P. H. (1975). High resolution two-dimensional electrophoresis of proteins.Journal of Biological Chemistry, 250, 4007–4021.Google Scholar
- Quaranta, S., Giuffrida, M. G., Cavaletto, M., Giunta, C., Godovac-Zimmermann, J., Cañas, B., Fabris, C., Bertino, E., Mombrò, M., & Conti, A. (2001). Human proteome enhancement: High-recovery method and improved two-dimensional map of colostral fat globule membrane proteins. Electrophoresis, 22, 1810–1818.CrossRefGoogle Scholar
- Sztalryd, C., Bell, M., Lu, X., Mertz, P., Hickenbottom, S., Chang, B. H. J., Chan, L., Kimmel, A. R., & Londos, C. (2006). Functional compensation for adipose differentiation-related protein (ADFP) by Tip47 in an ADFP null embryonic cell line. Journal of Biological Chemistry, 281, 34341–34348.CrossRefGoogle Scholar
- Wooding, F. B. P. (1971). The mechanism of secretion of the milk fat globule. Journal of Cell Science, 9, 805–821.Google Scholar