Monocotyledonous plants comprise the majority of agricultural plants in terms of biomass produced. Estimates of the number of species within this group range from 50,000 to 60,000. By far the largest monocot family is the orchid family, with some 20,000 species. Economically the most important family in this group (and in the flowering plants) is the grasses, family Gramineae or Poacea. Since many monocots are propagated through seed and monocot seed is often orthodox (can be dried to low moisture content see Chap. 19), seed conservation is the method of choice to store the diversity of many members of this group. Storage of desiccated seeds at low temperature is not applicable to crops that do not produce seed (e.g., bananas) or that produce recalcitrant seed (i.e. seed that can not be dried, see Chaps. 10, 18). Other plant species are propagated vegetatively to preserve the unique genomic constitution of cultivars (for crops such as yam, taro and garlic and ornamental plants such as lily and orchids) Vegetative tissues can be preserved in field collections and in vitro. Cryo- preservation, however, is the ultimate preservation method since under these conditions material can be preserved for unlimited periods without alteration. In this chapter cryopreservation protocols for vegetative tissues of monocotyledonous plants will be discussed.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abernethy DJ, Conner AJ (1992) Laboratory guide for the micropropagation of Asparagus. Crop and Food Research Report 1. Lincoln, NZ Inst Crop Food Res Ltd. pp. 34.Google Scholar
  2. Agrawal A, Swennen R, Panis B (2004) A comparison of four methods for cryopreservation of meristems in banana (Musa spp.). CryoLetters 25: 101-110.Google Scholar
  3. Baek HJ, Kim HH, Cho EG, Chae YA, Engelmann F (2003) Importance of explant size and origin and of preconditioning treatments for cryopreservation of garlic shoot apices by vitrification. CryoLetters 24: 381-388.PubMedGoogle Scholar
  4. Bouman H, Tiekstra A, Petutschnig E, Homan M, Schreurs R (2003) Cryopreservation of Lilium species and cultivars. In: Forkmann G, Hauser B, Michaelis S (eds.) Proceedings of the 21st International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals. Acta Hort 612: 147-154.Google Scholar
  5. Chang Y, Barker RE, Reed BM (2000) Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.). CryoLetters 21: 107-116.PubMedGoogle Scholar
  6. Dereuddre J, Scottez C, Arnaud Y, Duron M (1990) Resistance of alginate-coated axillary shoot tips of pear tree (Pyrus communis L. cv. Beurre Hardy) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen. C R Acad Sci Paris 310: 317-323.Google Scholar
  7. Ellis D, Skogerboe D, Andre C, Hellier B, Volk G (2006) Implementation of garlic cryopreservation techniques in the National Plant Germplasm System. CryoLetters 27: 99-106.PubMedGoogle Scholar
  8. Finkle BJ, Ulrich JM (1979) Effects of cryoprotectants in combination on the survival of frozen sugarcane cells. Plant Physiol 63: 598-604.CrossRefPubMedGoogle Scholar
  9. Gamborg O, Miller R, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158.CrossRefPubMedGoogle Scholar
  10. Gamez-Pastrana R, Martinez-Ocampo Y, Beristain CI, González-Arnao M (2004) An improved cryopreservation protocol for pineapple apices using encapsulation-vitrification. CryoLetters 25: 405-414.PubMedGoogle Scholar
  11. González-Arnao MT, Engelmann F, Huet C, Urra C (1993) Cryopreservation of encapsulated apices of sugarcane: Effect of freezing procedure and histology. CryoLetters 14: 303-308.Google Scholar
  12. González-Arnao MT, Moreira T, Urra C (1996) Importance of pregrowth with sucrose and vitrification for the cryopreservation of sugarcane apices using encapsulation-dehydration. CryoLetters 17: 141-148.Google Scholar
  13. González-Arnao M, Ravelo MM, Villavicencio CU, Martinez Montero MM, Engelmann F (1998) Cryopreservation of pineapple (Ananas comosus) apices by vitrification. CryoLetters 19: 375-382.Google Scholar
  14. González-Arnao MT, Urra C, Engelmann F, Ortiz R, de la Fe C (1999) Cryopreservation of encapsulated sugarcane apices: Effect of storage temperature and storage duration. CryoLetters 20: 347-352.Google Scholar
  15. Helliot B, Panis B, PouMay Y, Swennen R, Lepoivre P, Frison E (2002) Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Rept 20: 1117-1122.CrossRefGoogle Scholar
  16. Jitsuyama Y, Suzuki T, Harada T, Fujikawa S (2002) Sucrose incubation increases freezing tolerance of Asparagus (Asparagus officinalis L.) embryogenic cell suspensions. CryoLetters 23: 103-112.PubMedGoogle Scholar
  17. Keller ERJ (2002) Cryopreservation of Allium sativum L. (Garlic). In: Towill LE, Bajaj YPS (eds.) Biotechnology in Agriculture and Forestry. Vol 50 Cryopreservation of Plant Germplasm II. Springer, Heidelberg pp. 37-47.Google Scholar
  18. Keller ERJ (2005) Improvement of cryopreservation results in garlic using low temperature preculture and high-quality in vitro plantlets. CryoLetters 26: 357-366.PubMedGoogle Scholar
  19. Kim HH, Cho EG, Baek HJ, Kim CY, Keller ERJ, Engelmann F (2004a) Cryopreservation of garlic shoot tips by vitrification: Effects of dehydration, rewarming, unloading and regrowth conditions. CryoLetters 25: 59-70.PubMedGoogle Scholar
  20. Kim HH, Kim JB, Baek HJ, Cho EG, Chae YA, Engelmann F (2004b) Evolution of DMSO concentration in garlic shoot tips during a vitrification procedure. CryoLetters 25: 91-100.Google Scholar
  21. Kim HH, Yoon JW, Kim JB, Engelmann F, Cho EG (2005) Thermal analysis of garlic shoot tips during a vitrification procedure. CryoLetters 26: 33-44.PubMedGoogle Scholar
  22. Kohmura H, Sakai A, Chokyu S, Yakuwa T (1992) Cryopreservation of in vitrocultured multiple bud clusters of Asparagus (Asparagus officinalis L. cv Hiroshima green (2n = 30) by the techniques of vitrification. Plant Cell Rept 11: 433-437.Google Scholar
  23. Kyesmu PM, Takagi H (2000) Cryopreservation of shoot apices of yams (Dioscorea species) by vitrification. Engelmann F, Takagi H (eds.). Cryopreservation of Tropical Plant Germplasm. Current Research Progress and Applications. IPGRI, Rome, Italy pp. 420-422.Google Scholar
  24. Leunufna S, Keller ERJ (2003) Investigating a new cryopreservation protocol for yams (Dioscorea spp.). Plant Cell Rept 21: 1159-1166.CrossRefGoogle Scholar
  25. Leunufna S, Keller ERJ (2005) Cryopreservation of yams using vitrification modi-fied by including droplet method: Effects of cold acclimation and sucrose. CryoLetters 26: 93-102.PubMedGoogle Scholar
  26. Makowska Z, Keller J, Engelmann F (1999) Cryopreservation of apices isolated from garlic (Allium sativum L.) bulbils and cloves. CryoLetters 20: 175-182.Google Scholar
  27. Malaurie B, Trouslot M-F, Berthaud J, Bousalem M, Pinel A, Dubern J (1998a) Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm. EJB Electron J Biotechnol 1: 103-117. http://www.ejb.org.
  28. Malaurie B, Trouslot M-F, Engelmann F, Chabrillange N (1998b) Effect of pretreatment conditions on the cryopreservation of in vitro-cultured yam (Dioscorea alata ‘Brazo Fuerte’ and D. bulbifera ‘Nouméa Imboro’) shoot apices by encapsulation-dehydration. CryoLetters 19: 15-26.Google Scholar
  29. Mandal BB (2000) Cryopreservation of yam apices: A comparative study with three different techniques. Engelmann F, Takagi H (eds.). Cryopreservation of Tropical Plant Germplasm. Current Research Progress and Applications. IPGRI, Rome, Italy, pp. 233-237.Google Scholar
  30. Mandal BB, Chandel KPS, Dwivedi S (1996) Cryopreservation of yam (Dioscorea spp.) shoot apices by encapsulation-dehydration. CryoLetters 17: 165-174.Google Scholar
  31. Martinez Montero MM, González-Arnao MT, Martínez J, Engelmann F (2002) Application of cryopreservation techniques on pineapple Ananas comosus (L.) Merr. apices and calluses. Pineapple News 9: 17.Google Scholar
  32. Matsumoto T, Sakai A (1995) An approach to enhance dehydration tolerance of alginate-coated dried meristems cooled to −196°C. CryoLetters 16: 299-306.Google Scholar
  33. Matsumoto T, Sakai A, Yamada K (1995) Cryopreservation of in vitro-grown apical meristems of lily by vitrification. Plant Cell Tiss Organ Cult 41: 237-241.CrossRefGoogle Scholar
  34. Mix-Wagner G, Conner AJ, Cross RJ (2000) Survival and recovery of asparagus shoot tips after cryopreservation using the “droplet” method. N Z J Crop Hort Sci 28: 283-287.Google Scholar
  35. Morel G (1960) Producing virus-free Cymbidium. Amer Orchid Soc Bull 12: 77-79.Google Scholar
  36. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.CrossRefGoogle Scholar
  37. Na HY, Kondo K (1996) Cryopreservation of tissue-cultured shoot primordia from shoot apices of cultured protocorms in Vanda pumila following ABA preculture and desiccation. Plant Sci 118: 195-201.CrossRefGoogle Scholar
  38. Niwata E (1995) Cryopreservation of apical meristems of garlic (Allium sativum L.) and high subsequent plant regeneration. CryoLetters 16: 102-107.Google Scholar
  39. Panis B (1995) Cryopreservation of banana (Musa spp.) germplasm. Dissertationes de Agricultura (272). Katholieke Universiteit Leuven, Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, pp. 201.Google Scholar
  40. Panis B, Lambardi M (2005) Status of cryopreservation technologies in plants (crops and forest trees). International Workshop on “The role of biotechnology for the characterisation and conservation of crop, forestry, animal and fishery genetic resources”. Turin, Italy, pp. 43-54.Google Scholar
  41. Panis B, Piette B, Swennen R (2005) Droplet vitrification of apical meristems: A cryopreservation protocol applicable to all Musaceae. Plant Sci 168: 45-55.CrossRefGoogle Scholar
  42. Panis B, Strosse H, Van Den Hende S, Swennen R (2002) Sucrose preculture to simplify cryopreservation of banana meristem cultures. CryoLetters 23: 375-384.PubMedGoogle Scholar
  43. Panis B, Swennen R, Engelmann F (2001) Cryopreservation of plant germplasm. Acta Hort 560: 79-86.Google Scholar
  44. Panis B, Thinh NT (2001) Cryopreservation of Musa germplasm. In: Escalant JV, Sharrock S (eds.). INIBAP Technical Guidelines 5. International Network for the Improvement of Banana and Plantain, Montpellier, France, pp. 44 http://www.inibap.org/pdf/IN020224_en.pdf.
  45. Panis B, Totte N, Vannimmen K, Withers LA, Swennen R (1996) Cryopreservation of banana (Musa Spp) meristem cultures after preculture on sucrose. Plant Sci 121: 95-106.CrossRefGoogle Scholar
  46. Paulet F, Engelmann F, Glaszmann JC (1993) Cryopreservation of apices of in vitro plantlets of sugarcane (Saccharum sp hybrids) using encapsulation/dehydration. Plant Cell Rep 12: 525-952.CrossRefGoogle Scholar
  47. Reed BM, Schumacher L, Wang N, D’Achino J, Barker RE (2006) Cryopreservation of bermudagrass germplasm by encapsulation dehydration. Crop Sci 46: 6-11.CrossRefGoogle Scholar
  48. Sakai A, Kobayashi S, Oiyama I. (1990) Cryopreservation of nucellar cells of navel orange (Citrus-Sinensis Osb Var Brasiliensis Tanaka) by vitrification. Plant Cell Rep 9: 30-33.CrossRefGoogle Scholar
  49. Schäfer-Menuhr A, Müller E, Mix-Wagner G (1996) Cryopreservation: An alternative for the long-term storage of old potato varieties. Potato Res 39: 507-513.CrossRefGoogle Scholar
  50. Strosse H, Domergue R, Panis B, Escalant JV, Côte F (2003) Banana and plantain embryogenic cell suspensions. In: Vézina A, Picq C (eds.). INIBAP Technical Guidelines 8. INIBAP, Montpellier, France pp 31 http://www.inibap.org/pdf/IN040511_en.pdf.
  51. Strosse H, Schoofs H, Panis B, André E, Reyniers K, Swennen R (2006) Development of embryogenic cell suspensions from shoot meristematic tissue in bananas and plantains (Musa spp.). Plant Sci 170: 104-112.CrossRefGoogle Scholar
  52. Suzuki T, Kaneko M, Harada T (1997) Increase in freezing resistance of excised shoot tips of Asparagus officinalis L. by preculture on sugar-rich media. Cryobiology 34: 264-275.CrossRefGoogle Scholar
  53. Suzuki T, Kaneko M, Harada T, Yakuwa T (1998) Enhanced formation of roots and subsequent promotion of growth of shoots on cryopreserved nodal segments of Asparagus officinalis L. Cryobiology 36: 194-205.CrossRefPubMedGoogle Scholar
  54. Takagi H, Thinh N, Islam O, Senboku T, Sakai A (1997) Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification.1. Investigation of basic conditions of the vitrification procedure. Plant Cell Rep 16: 594-599.CrossRefGoogle Scholar
  55. Thinh NT, Takagi H (2000) Cryopreservation of in vitro-grown apical meristems of terrestrial orchids (Cymbidium spp.) by vitrification. In: Engelmann F, Takagi H (eds.). Cryopreservation of Tropical Plant Germplasm, Current Research Progress and Applications. IPGRI, Rome, Italy, pp 453-455.Google Scholar
  56. Thinh NT, Takagi H, Yashima S (1999) Cryopreservation of in vitro-grown shoot tips of banana (Musa spp.) by vitrification method. CryoLetters 20: 163-174.Google Scholar
  57. Turner S, Senaratna T, Bunn E, Tan B, Dixon KW, Touchell DH (2001a) Cryopreservation of shoot tips from six endangered Australian species using a modified vitrification protocol. Ann Bot 87: 371-378.CrossRefGoogle Scholar
  58. Turner SR, Touchell DH, Senaratna T, Bunn E, Tan B, Dixon KW (2001b) Effects of plant growth regulators on survival and recovery growth following cryopreservation. CryoLetters 22: 163-174.PubMedGoogle Scholar
  59. Uragami A, Sakai A, Nagai M (1990) Cryopreservation of dried axillary buds from plantlets of Asparagus officinalis L. grown in-vitro. Plant Cell Rep 9: 328-331.CrossRefGoogle Scholar
  60. Volk GM, Maness N, Rotindo K (2004) Cryopreservation of garlic (Allium sativum L.) using plant vitrification solution 2. CryoLetters 25: 219-226.PubMedGoogle Scholar
  61. Wimber DD (1963) Clonal multiplication of Cymbidium through tissue culture of the shoot meristem. Am Orchid Soc Bull 32: 105-107.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bart Panis
    • 1
  1. 1.Laboratory of Tropical Crop ImprovementCatholic University of LeuvenBelgium

Personalised recommendations