Neural Crest Stem Cells

Abstract

Stem cells are defined by their ability to both self-renew and give rise to multiple lineages in vivo and/or in vitro. As discussed in other chapters in this volume, the embryonic neural crest is a multipotent tissue that gives rise to a plethora of differentiated cell types in the adult organism and is unique to vertebrate embryos. From the point of view of stem cell biology, the neural crest is an ideal source for multipotent adult stem cells. Significant advances have been made in the past few years isolating neural crest stem cell lines that can be maintained in vitro and can give rise to many neural crest derivatives either in vitro or when placed back into the context of an embryo. The initial work identifying these stem cells was carried out with premigratory neural crest from the embryonic neural tube. Later, neural crest stem cells were isolated from postmigratory neural crest, presumably more restricted in developmental potential. More recently it has been demonstrated that neural crest stem cell progenitors persist in the adult in at least two differentiated tissues, the enteric nervous system of the gut and the whisker follicles of the facial skin. In all cases, the properties of the stem cells derived reflect their tissue of origin and the potential of the progenitors becomes more restricted with age. In this chapter we will review this work and speculate on future possibilities with respect to combining our knowledge of neural crest gene function in the embryo and the manipulation of adult neural crest stem cells in vitro and eventually in vivo.

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References

  1. 1.
    Stemple DL, Anderson DJ. Isolation of a stem cell for neurons and glia from the mammalian neural crest. Cell 1992;71(6):973–985.PubMedCrossRefGoogle Scholar
  2. 2.
    Dechant G, Barde YA. Signalling through the neurotrophin receptor p75NTR. Curr Opin Neurobiol 1997;7(3):413–418.PubMedCrossRefGoogle Scholar
  3. 3.
    Shah NM, Groves AK, Anderson DJ. Alternative neural crest cell fates are instructively promoted by TGFbeta superfamily members. Cell 1996;85(3):331–343.PubMedCrossRefGoogle Scholar
  4. 4.
    Shah NM, Anderson DJ. Integration of multiple instructive cues by neural crest stem cells reveals cell-intrinsic biases in relative growth factor responsiveness. Proc Natl Acad Sci USA 1997;94(21):11369–11374.PubMedCrossRefGoogle Scholar
  5. 5.
    Morrison SJ, White PM, Zock C et al. Prospective identification, isolation by flow cytometry, and in vivo self-renewal of multipotent mammalian neural crest stem cells. Cell 1999;96(5):737–749.PubMedCrossRefGoogle Scholar
  6. 6.
    White PM, Morrison SJ, Orimoto K et al. Neural crest stem cells undergo cell-intrinsic developmental changes in sensitivity to instructive differentiation signals. Neuron 2001;29(1):57–71.PubMedCrossRefGoogle Scholar
  7. 7.
    Erlebacher A, Price KA, Glimcher LH. Maintenance of mouse trophoblast stem cell proliferation by TGF-beta/activin. Dev Biol 2004;275(1):158–169.PubMedCrossRefGoogle Scholar
  8. 8.
    Guzman-Ayala M, Ben-Haim N, Beck S et al. Nodal protein processing and fibroblast growth factor 4 synergize to maintain a trophoblast stem cell microenvironment. Proc Natl Acad Sci USA 2004;101(44):15656–15660.PubMedCrossRefGoogle Scholar
  9. 9.
    Qi X, Li TG, Hao J et al. BMP4 supports self-renewal of embryonic stem cells by inhibiting mitogen-activated protein kinase pathways. Proc Natl Acad Sci USA 2004;101(16):6027–6032.PubMedCrossRefGoogle Scholar
  10. 10.
    Ying QL, Nichols J, Chambers I et al. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 2003;115(3):281–292.PubMedCrossRefGoogle Scholar
  11. 11.
    Kanzler B, Foreman RK, Labosky PA et al. BMP signaling is essential for development of skeletogenic and neurogenic cranial neural crest. Development 2000;127(5):1095–1104.PubMedGoogle Scholar
  12. 12.
    Stottmann RW, Choi M, Mishina Y et al. BMP receptor LA is required in mammalian neural crest cells for development of the cardiac outflow tract and ventricular myocardium. Development 2004;131(9):2205–2218.PubMedCrossRefGoogle Scholar
  13. 13.
    Bixby S, Kruger GM, Mosher JT et al. Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity. Neuron 2002;35(4):643–656.PubMedCrossRefGoogle Scholar
  14. 14.
    Kubu CJ, Orimoto K, Morrison SJ et al. Developmental changes in Notch1 and numb expression mediated by local cell-cell interactions underlie progressively increasing delta sensitivity in neural crest stem cells. Dev Biol 2002;244(1):199–214.PubMedCrossRefGoogle Scholar
  15. 15.
    Iwashita T, Kruger GM, Pardal R et al. Hirschsprung disease is linked to defects in neural crest stem cell function. Science 2003;301(5635):972–976.PubMedCrossRefGoogle Scholar
  16. 16.
    Britsch S, Goerich DE, Riethmachcr D et al. The transcription factor Sox10 is a key regulator of peripheral glial development. Genes Dev 2001;15(1):66–78.PubMedCrossRefGoogle Scholar
  17. 17.
    Kim J, Lo L, Dormand E et al. SOX10 maintains multipotency and inhibits neuronal differentiation of neural crest stem cells. Neuron 2003;38(1):17–31.PubMedCrossRefGoogle Scholar
  18. 18.
    Paratore C, Brugnoli G, Lee HY et al. The role of the Ets domain transcription factor Erm in modulating differentiation of neural crest stem cells. Dev Biol 2002;250(1):168–180.PubMedCrossRefGoogle Scholar
  19. 19.
    Soo K, O’Rourke MP, Khoo PL et al. Twist function is required for the morphogenesis of the cephalic neural tube and the differentiation of the cranial neural crest cells in the mouse embryo. Dev Biol 2002;247(2):251–270.PubMedCrossRefGoogle Scholar
  20. 20.
    Carver EA, Jiang R, Lan Y et al. The mouse snail gene encodes a key regulator of the epithelial-mesenchymal transition. Mol Cell Biol 2001;21(23):8184–8188.PubMedCrossRefGoogle Scholar
  21. 21.
    Hanna LA, Foreman RK, Tarasenko IA et al. Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo. Genes Dev 2002;16(20):2650–2661.PubMedCrossRefGoogle Scholar
  22. 22.
    Jiang R, Lan Y, Norton CR et al. The Slug gene is not essential for mesoderm or neural crest development in mice. Dev Biol 1998;198(2):277–285.PubMedGoogle Scholar
  23. 23.
    Lee HY, Kleber M, Hari L et al. Instructive role of Wnt/beta-catenin in sensory fate specification in neural crest stem cells. Science 2004;303(5660):1020–1023.PubMedCrossRefGoogle Scholar
  24. 24.
    Kleber M, Lee HY, Wurdak H et al. Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling. J Cell Biol 2005;169(2):309–320.PubMedCrossRefGoogle Scholar
  25. 25.
    Kruger GM, Mosher JT, Bixby S et al. Neural crest stem cells persist in the adult gut but undergo changes in self-renewal, neuronal subtype potential, and factor responsiveness. Neuron 2002;35(4):657–669.PubMedCrossRefGoogle Scholar
  26. 26.
    Fernandes KJ, McKenzie IA, Mill P et al. A dermal niche for multipotent adult skin-derived precursor cells. Nat Cell Biol 2004;6(11):1082–1093.PubMedCrossRefGoogle Scholar
  27. 27.
    Toma JG, Akhavan M, Fernandes KJ et al. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 2001;3(9):778–784.PubMedCrossRefGoogle Scholar
  28. 28.
    Toma JG, McKenzie IA, Bagli D et al. Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells 2005;23(6):727–737.PubMedCrossRefGoogle Scholar
  29. 29.
    Chai Y, Jiang X, Ito Y et al. Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. Development 2000;127(8):1671–1679.PubMedGoogle Scholar
  30. 30.
    Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 1999;21(1):70–71.PubMedCrossRefGoogle Scholar
  31. 31.
    Sieber-Blum M, Grim M, Hu YF et al. Pluripotent neural crest stem cells in the adult hair follicle. Dev Dyn 2004;231(2):258–269.PubMedCrossRefGoogle Scholar
  32. 32.
    Miura M, Gronthos S, Zhao M et al. SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA 2003;100(10):5807–5812.PubMedCrossRefGoogle Scholar

Copyright information

© Landes Bioscience and Springer Science+Business Media 2006

Authors and Affiliations

  1. 1.Department of Cell and Developmental BiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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