The Contrast Formation in Optical Microscopy

  • Ping-Chin Cheng


In any form of microscopy, one needs not only an imaging system with enough resolution to delineate the fine details of the specimen but also a suitable contrast mechanism by which to “see” the shape of the structures of interest. Contrast is the difference between the signal in one pixel and that in another that conveys to the viewer information about the shape of the specimen. It is the difference between a blank screen and an image.


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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Ping-Chin Cheng
    • 1
    • 2
  1. 1.State University of New York at BuffaloBuffalo
  2. 2.National University of SingaporeSingapore

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