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Complementary Techniques

Validation of Gene Expression Data by Quantitative Real Time PCR
  • Maurizio Provenzano
  • Simone Mocellin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 593)

Abstract

Microarray technology can be considered the most powerful tool for screening gene expression profiles of biological samples. After data mining, results need to be validated with highly reliable biotechniques allowing for precise quantitation of transcriptional abundance of identified genes. Quantitative real time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstrumentation for gene level measurement. Currently, qrt-PCR is considered by most experts the most appropriate method to confirm or confute microarray-generated data. The knowledge of the biochemical principles underlying qrt-PCR as well as some related technical issues must be beard in mind when using this biotechnology.

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Copyright information

© Landes Bioscience and Springer Science+Business Media 2007

Authors and Affiliations

  1. 1.Department of Oncological and Surgical SciencesUniversity of PadovaPadovaItaly

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