Dissection of the Fusion Machine of Sars-Coronavirus

  • Megan W. Howard
  • Brian Tripet
  • Michael G. Jobling
  • Randall K. Holmes
  • Kathryn V. Holmes
  • Robert S. Hodges
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 581)


Severe Acute Respiratory Syndrome Coiled Coil Severe Acute Respiratory Syndrome Heptad Repeat Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    L. J. Earp, S. E. Delos, H. E. Park, and J. M. White, The many mechanisms of viral membrane fusion proteins, Curr. Top. Microbiol. Immunol. 285, 25–66 (2004).CrossRefGoogle Scholar
  2. 2.
    T. C. Nash and M. J. Buchmeier, Entry of mouse hepatitis virus into cells by endosomal and nonendosomal pathways, Virology 233, 1–8 (1997).CrossRefPubMedGoogle Scholar
  3. 3.
    B. D. Zelus, J. H. Schickli, D. M. Blau, S. R Weiss, and K. V. Holmes, Conformational changes in the spike glycoprotein of murine coronavirus are induced at 37 degrees C either by soluble murine CEACAM1 receptors or by pH 8, J. Virol. 77, 830–840 (2003).CrossRefPubMedGoogle Scholar
  4. 4.
    G. S. Dveksler, C. W. Dieffenbach, C. B. Cardellichio, K. McCuaig, M. N. Pensiero, G. S. Jiang, N. Beauchemin, and K. V. Holmes, Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for the coronavirus mouse hepatitis virus-A59, J. Virol. 67, 1–8 (1993).PubMedGoogle Scholar
  5. 5.
    B. J. Bosch, R. van der Zee, C. A. de Haan, and P. J. Rottier, The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex, J. Virol. 77, 8801–8811 (2003).CrossRefPubMedGoogle Scholar
  6. 6.
    K. A. Baker, R. E. Dutch, R A. Lamb, and T. S. Jardetzky, Structural basis for paramyxovirus-mediated membrane fusion, Mol. Cell 3, 309–319 (1999).CrossRefPubMedGoogle Scholar
  7. 7.
    P. M. Colman and M. C. Lawrence, The structural biology of type I viral membrane fusion, Nat. Rev. Mol. Cell Biol. 4, 309–319 (2003).CrossRefPubMedGoogle Scholar
  8. 8.
    B. Tripet and R. S. Hodges, in Peptides: The Wave of the Future, edited by M. Lebl and R. A. Houghten (American Peptide Society, San Diego, 2001), pp. 365–366.Google Scholar
  9. 9.
    B. Tripet, M. W. Howard, M. Jobling, R. K. Holmes, K. V. Holmes, and R. S. Hodges, Structural characterization of the SARS-coronavirus spike S fusion protein core, J. Biol. Chem. 279, 20836–20849 (2004).CrossRefPubMedGoogle Scholar
  10. 10.
    B. J. Bosch, B. E. Martina, R. van der Zee, J. Lepault, B. J. Haijema, C. Versluis, A. J. Heck, R. De Groot, A. D. Osterhaus, and P. J. Rottier, Severe acute respiratory syndrome coronavirus (SARS-CoV) infection inhibition using spike protein heptad repeat-derived peptides, Proc. Natl. Acad. Sci. USA 101, 8455–8460 (2004).CrossRefPubMedGoogle Scholar
  11. 11.
    Y. Xu, Z. Lou, Y. Liu, H. Pang, P. Tien, G. F. Gao, and Z. Rao, Crystal structure of severe acute respiratory syndrome coronavirus spike protein fusion core, J. Biol. Chem. 279, 49414–49419 (2004).CrossRefPubMedGoogle Scholar
  12. 12.
    S. Liu, G. Xiao, Y. Chen, Y. He, J. Niu, C. R. Escalante, H. Xiong, J. Farmar, A. K. Debnath, P. Tien, and S. Jiang, Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification of fusion inhibitors, Lancet 363, 938–947 (2004).CrossRefPubMedGoogle Scholar
  13. 13.
    Y. Xu, J. Zhu, Y. Liu, Z. Lou, F. Yuan, Y. Liu, D. K. Cole, L. Ni, N. Su, L. Qin, X. Li, Z. Bai Z., J. I. Bell, H. Pang, P. Tien, G. F. Gao, and Z. Rao, Characterization of the heptad repeat regions, HR1 and HR2, and design of a fusion core structure model of the spike protein from severe acute respiratory syndrome (SARS) coronavirus, Biochemistry 43, 14064–14074 (2004).CrossRefPubMedGoogle Scholar
  14. 14.
    K. Yuan, L. Yi, J. Chen, X. Qu, T. Qing, X. Rao, P. Jiang, J. Hu, Z. Xiong, Y. Nie, X. Shi, W. Wang, C. Ling, X. Yin, K. Fan, L. Lai, M. Ding, and H. Deng, Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein, Biochem. Biophys. Res. Commun. 319, 746–752 (2004).CrossRefPubMedGoogle Scholar
  15. 15.
    P. Ingallinella, E. Bianchi, M. Finotto, G. Cantoni, D. M. Eckert, V. M. Supekar, C. Bruckmann, A. Carfi, and A. Pessi, Structural characterization of the fusion-active complex of severe acute respiratory syndrome (SARS) coronavirus, Proc. Natl. Acad. Sci. USA 101, 8709–8714 (2004).CrossRefPubMedGoogle Scholar
  16. 16.
    Y. Xu, Z. Bai, L. Qin, X. Li, G. Gao, and Z. Rao, Crystallization and preliminary crystallographic analysis of the fusion core of the spike protein of the murine coronavirus mouse hepatitis virus (MHV), Acta Crystallogr. D 60, 2013–2015 (2004).CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Megan W. Howard
    • 1
  • Brian Tripet
    • 2
  • Michael G. Jobling
    • 3
  • Randall K. Holmes
    • 4
  • Kathryn V. Holmes
    • 5
  • Robert S. Hodges
    • 6
  1. 1.University of Colorado Health Sciences CenterAuroraUSA
  2. 2.University of Colorado Health Sciences CenterAuroraUSA
  3. 3.University of Colorado Health Sciences CenterAuroraUSA
  4. 4.Department of MicrobiologyUniversity of Colorado Health Sciences CenterAuroraUSA
  5. 5.Department of MicrobiologyUniversity of Colorado Health Sciences CenterAuroraUSA
  6. 6.University of Colorado Health Sciences CenterAuroraUSA

Personalised recommendations