Diet, Immunity and Functional Foods

  • Lesley Hoyles
  • Jelena Vulevic


Functional foods (specific nutrient and/or food components) should beneficially affect one or more target functions in the body. The use of functional foods as a form of preventive medicine has been the subject of much research over the last two decades. It is well known that nutrition plays a vital role in chronic diseases, but it is only recently that data relating to the effects of specific nutrients or foods on the immune system have become available. This chapter aims to summarize the effects of some functional foods (e.g., prebiotics and micronutrients) on the immune system. It should be noted, however, that studies into the role of functional foods with regard to the human immune system are still in their infancy and a great deal of controversy surrounds the health claims attributed to some functional foods. Consequently, thorough studies are required in human and animal systems if we are to move towards developing a functional diet that provides maximal health benefits.


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  1. 1.
    Roberfroid MB. Defining functional foods. In: Gibson GR, Williams CM, eds. Functional Foods: Concept to Product. Boca Raton: CRC Press LLC, 2000:9–27.Google Scholar
  2. 2.
    Diplock AT, Agget PJ, Ashwell M et al. Scientific concepts of functional foods in Europe: consensus document. Br J Nutr 1999; 81(suppl):S1–S28.Google Scholar
  3. 3.
    Calder PC, Kew S. The immune system: a target for functional foods? Br J Nutr 2002; 88(suppl): S165–S176.PubMedCrossRefGoogle Scholar
  4. 4.
    Calder PC, Field CJ, Gill HS, eds. Nutrition and Immune Function. Wallingford: CABI Publishing, 2002.Google Scholar
  5. 5.
    Johnson IT. New food components and gastrointestinal health. Proc Nutr Soc 2001; 60:481–485.PubMedCrossRefGoogle Scholar
  6. 6.
    Mollet B, Rowland I. Functional foods: at the frontier between food and pharma. Editorial overview. Curr Opin Biotechnol 2002; 13:483–485.PubMedCrossRefGoogle Scholar
  7. 7.
    Gibson GR, Roberfroid MB, eds. Colonic Microbiota, Nutrition and Health. Dordrecht: Kluwer Academic Publishers, 1999.Google Scholar
  8. 8.
    Fuller R. Probiotics: growth-promoting factors produced by microorganisms. Science 1989; 147: 747–748.Google Scholar
  9. 9.
    Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 1995; 125:1401–1412.PubMedGoogle Scholar
  10. 10.
    Shortt C. Living it up for dinner. Chem Ind 1998; 8:300–303.Google Scholar
  11. 11.
    Metchnikoff, E. The Prolongation of Life: Optimistic Studies. New York: GP Putnam’s Sons, 1908.Google Scholar
  12. 12.
    Molis C, Florie B, Ouarne F et al. Digestion, excretion and energy value of fructooligosaccharides in healthy humans. Am J Clin Nutr 1996; 64:324–328.PubMedGoogle Scholar
  13. 13.
    Gibson GR, Probert HM, Loo JV et al. Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutr Res Rev 2004; 17:259–275.PubMedCrossRefGoogle Scholar
  14. 14.
    Morrow AL, Guerrero ML, Shults J et al. Efficacy of home-based peer counselling to promote exclusive breastfeeding: a randomised controlled trial. Lancet 1999; 353:1226–1231.PubMedCrossRefGoogle Scholar
  15. 15.
    Newburg DS, Ruiz-Palacios GM, Morrow AL. Human milk glycans protect infants against enteric pathogens. Annu Rev Nutr 2005; 25:37–58.PubMedCrossRefGoogle Scholar
  16. 16.
    Buddington KK, Donahoo JB, Buddington RK. Dietary oligofructose and inulin protect mice from enteric and systemic pathogens and tumor inducers. J Nutr 2002; 132:472–477.PubMedGoogle Scholar
  17. 17.
    Kelly-Quagliana KA, Nelson PD, Buddington RK. Dietary oligofructose and inulin modulate immune function in mice. Nutr Res 2003; 23:257–267.CrossRefGoogle Scholar
  18. 18.
    Pierre F, Perrin P, Champ M et al. Short-chain fructo-oligosaccharides reduce the occurrence of colon tumors and develop gut-associated lymphoid tissue in Min mice. Cancer Res 1997; 57:225–228.PubMedGoogle Scholar
  19. 19.
    Pierre F, Perrin P, Bassonga E et al. T-cell status influences colon tumor occurrence in Min mice fed short-chain fructo-oligosaccharides as a diet supplement. Carcinogenesis 1999; 20:1953–1956.PubMedCrossRefGoogle Scholar
  20. 20.
    Hosono A, Ozawa A, Kato R et al. Dietary fructooligosaccharides induce immunoregulation of intestinal IgA secretion by murine Peyer’s patch cells. Biosci Biotechnol Biochem 2003; 67:758–764.PubMedCrossRefGoogle Scholar
  21. 21.
    Nakamura Y, Nosaka S, Suzuki M et al. Dietary fructooligosaccharides up-regulate immunoglobulin A response and polymeric immunoglobulin receptor expression in intestines of infant mice. Clin Exp Immunol 2004; 137:52–58.PubMedCrossRefGoogle Scholar
  22. 22.
    Kudoh K, Shimizu J, Wada M et al. Effect of indigestible saccharides on B-lymphocyte response of intestinal mucosa and cecal fermentation in rats. J Nutr Sci Vit 1998; 44:103–112.Google Scholar
  23. 23.
    Nagendra R, Venkat Rao S. Effect of feeding infant formulations containing bifidus factors on in vivo proliferation of bifidobacteria and stimulation of intraperitoneal macrophage activity in rats. J Nutr Immunol 1994; 2:61–68.CrossRefGoogle Scholar
  24. 24.
    Manhart N, Spittler A, Bergmeister H et al. Influence of fructooligosaccharides on Peyer’s patch lymphocyte numbers in healthy and endotoxemic mice. Nutrition 2003; 19:657–660.PubMedCrossRefGoogle Scholar
  25. 25.
    Guigoz Y, Rochat F, Perruisseau-Carrier G et al. Effects of oligosaccharide on the faccal flora and nonspecific immune system in elderly people. Nutr Res 2002; 22:13–25.CrossRefGoogle Scholar
  26. 26.
    Bunout D, Hirsch S, de la Maza MP et al. Effects of prebiotics on the immune response to vaccination in the elderly. J Parenter Enter Nutr 2002; 26:372–376.CrossRefGoogle Scholar
  27. 27.
    Sazawal S, Dhingra U, Sarkar A et al. Efficacy of milk fortified with a probiotic Bifidobacterium lactis (DR-10TM) and prebiotic galacto-oligosaccharides in prevention of morbidity and on nutritional status. Asia Pac J Clin Nutr 2004; 13:S28.Google Scholar
  28. 28.
    Gibson GR, Beatty ER, Wang X et al. Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 1995; 108:975–982.PubMedCrossRefGoogle Scholar
  29. 29.
    Bouhnik Y, Flourie B, D’Agay-bensour L et al. Administration of transgalactooligosaccharides increase fecal bifidobacteria and modifies colonic fermentation metabolism in healthy humans. J Nutr 1997; 127:444–448.PubMedGoogle Scholar
  30. 30.
    Kleessen B, Hartmann L, Blaut M. Oligofructose and long-chain inulin: influence on the gut microbial ecology of rats associated with a human faccal flora. Br J Nutr 2001; 86:291–300.PubMedCrossRefGoogle Scholar
  31. 31.
    Vulevic J, Rastall RA, Gibson GR. Developing a quantitative approach for determining the in vitro prebiotic potential of dietary oligosaccharides. FEMS Microbiol Lett 2004; 236:153–159.PubMedCrossRefGoogle Scholar
  32. 32.
    Takahashi T, Nakagawa E, Nara T et al. Effects of orally ingested Bifidobacterium longum on the mucosal IgA response of mice to dietary antigens. Biosci Biotechnol Biochem 1998; 62:10–15.PubMedCrossRefGoogle Scholar
  33. 33.
    Tejada-Simon MV, Ustunol Z, Pestka JJ. Effects of lactic acid bacteria ingestion of basal cytokine mRNA and immunoglobulin levels in the mouse. J Food Prot 1999; 62:287–291.PubMedGoogle Scholar
  34. 34.
    Qiao H, Duffy LC, Griffiths E et al. Immune responses in rhesus rotavirus-challenged Balb/c mice treated with bifidobacteria and prebiotic supplements. Pediatr Res 2002; 51:750–755.PubMedGoogle Scholar
  35. 35.
    Moineau S, Goulet J. Effect of feeding fermented milks on the pulmonary macrophage activity in mice. Milchwissenschaft 1991; 46:551–554.Google Scholar
  36. 36.
    Matsuzaki T, Yamazaki R, Hashimoto S et al. The effect of oral feeding of Lactobacillus casei strain Shirota on immunoglobulin E production in mice. J Dairy Sci 1998; 81:48–53.PubMedCrossRefGoogle Scholar
  37. 37.
    Tejada-Simon MV, Ustunol Z, Pestka JJ. Ex vivo effects of lactobacilli, streptococci and bifidobacteria ingestion on cytokine and nitric oxide production in a murine model. J Food Prot 1999; 62:162–169.PubMedGoogle Scholar
  38. 38.
    Akira S, Uematsu S, Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124: 783–801.PubMedCrossRefGoogle Scholar
  39. 39.
    Vasselon T, Detmers PA. Toll receptors: a central element in innate immune responses. Infect Immun 2002; 70:1033–1041.PubMedCrossRefGoogle Scholar
  40. 40.
    Forchielli ML, Walker WA. The role of gut-associated lymphoid tissues and mucosal defence. Br J Nutr 2005; 93(Suppl):S41–S48.PubMedCrossRefGoogle Scholar
  41. 41.
    Hatcher GE, Lambrecht RS. Augmentation of macrophage phagocytic activity by cell-free extracts of selected lactic acid-producing bacteria. J Dairy Sci 1993; 76:2485–2492.PubMedCrossRefGoogle Scholar
  42. 42.
    Macfarlane GT, Cummings JH. The colonic flora, fermentation and large bowel digestive function. In: Phillips SF, Pemberton JH, Shorter RG eds. The Large Intestine: Physiology, Pathophysiology and Disease. New York: Raven Press Ltd, 1991; 51–92.Google Scholar
  43. 43.
    Salminen S, Bouley C, Boutron-Ruault MC et al. Functional food science and gastrointestinal physiology and function. Br J Nutr 1998; 80:S147–S171.PubMedCrossRefGoogle Scholar
  44. 44.
    Roediger WEW. Utilisation of nutrients by isolated epithelial cells of the rat colon. Gastroenterology 1982; 83:424–429.PubMedGoogle Scholar
  45. 45.
    Cavaglieri CR, Nishiyama A, Fernandes LC et al. Differential effects of short-chain fatty acids on proliferation and production of pro-and anti-inflammatory cytokines by cultured lymphocytes. Life Sci 2003; 73: 1683–1690.PubMedCrossRefGoogle Scholar
  46. 46.
    Zapolska-Downar D, Siennicka A, Kaczmarczyk M et al. Butyrate inhibits cytokine-induced VCAM-1 and ICAM-1 expression in cultured endothelial cells: the role of NF-κB and PPARα. J Nutr Biochem 2004; 15: 220–228.PubMedCrossRefGoogle Scholar
  47. 47.
    Kruh J, Defer N, Tichonicky L. Effects of butyrate on cell proliferation and gene expression. In: Cummings JH, Rombeau JL, Sakata T, eds. Physiological and Clinical Aspects of Short-Chain Fatty Acids. Cambridge: Cambridge University Press, 1995:275–288.Google Scholar
  48. 48.
    Hague A, Elder DJE, Hicks DJ et al. Apoptosis in colorectal tumour cells: induction by the short chain fatty acids butyrate, propionate and acetate and by the bile salt deoxycholate. Int J Cancer 1995; 60:400–406.PubMedCrossRefGoogle Scholar
  49. 49.
    Pratt VC, Tappenden KA, McBurney MI et al. Short-chain fatty acid-supplemented total parenteral nutrition improves nonspecific immunity after intestinal resection in rats. JPEN J Parenter Enteral Nutr 1996; 20:264–271.PubMedCrossRefGoogle Scholar
  50. 50.
    Ishizaka S, Kikuchi E, Tsujii T. Effects of acetate on human immune system. Immunopharmacol Immunotoxicol 1993; 15: 151–162.PubMedCrossRefGoogle Scholar
  51. 51.
    Wu GY, Field CJ, Marliss EB. Glutamine and glucose metabolism in rat splenocytes and mesenteric lymph node lymphocytes. Am J Physiol 1991; 260:E141–E147.PubMedGoogle Scholar
  52. 52.
    Jenkins DJ, Popovich DG, Kendall CW et al. Metabolic effects of non-absorbable carbohydrates. Scand J Gastroenterol 1999; 222: 10–13.Google Scholar
  53. 53.
    Jenkins DJ, Kendall CW, Vuksan V. Inulin, oligofructose and intestinal function. J Nutr 1999; 129 (suppl):1431S–1433S.PubMedGoogle Scholar
  54. 54.
    Deplancke B, Gaskins HR. Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. Am J Clin Nutr 2001; 73 (suppl):1131S–1141S.PubMedGoogle Scholar
  55. 55.
    Matsuo K, Ota H, Akamatsu T et al. Histochemistry of the surface mucous gel layer of the human colon. Gut 1997; 40: 782–789.PubMedCrossRefGoogle Scholar
  56. 56.
    Hoskins LC, Boulding ET. Mucin degradation in human colon ecosystems. Evidence for the existence and role of bacterial subpopulations producing glycosidases as extracellular enzymes. J Clin Invest 1981; 67:163–172.PubMedCrossRefGoogle Scholar
  57. 57.
    Fontaine N, Meslin JC, Lory S et al. Intestinal mucin distribution in the germ-free rat and in the heteroxenic rat harbouring a human bacterial flora: effect of inulin in the diet. Br J Nutr 1996; 75: 881–892.PubMedCrossRefGoogle Scholar
  58. 58.
    Frankel W, Zhang W, Singh A et al. Fiber: effect on bacterial translocation and intestinal mucin content. World J Surg 1995; 19: 144–149.PubMedCrossRefGoogle Scholar
  59. 59.
    Xu D, Lu Q, Deitch EA. Elemental diet-induced bacterial translocation associated with systemic and intestinal immune suppression. JPEN J Parenter Enteral Nutr 1998; 22: 37–41.PubMedCrossRefGoogle Scholar
  60. 60.
    Shimotoyodome A, Meguro S, Hase T et al. Short chain fatty acids but not lactate or succinate stimulate mucus release in the rat colon. Comp Biochem Physiol A Mol Integr Physiol 2000; 125:525–531.PubMedCrossRefGoogle Scholar
  61. 61.
    Finnie IA, Dwarakanath AD, Taylor BA et al. Colonic mucin synthesis is increased by sodium butyrate. Gut 1995; 36: 93–99.PubMedCrossRefGoogle Scholar
  62. 62.
    Gaudier E, Jarry A, Blottiere HM et al. Butyrate specifically modulates MUC gene expression in intestinal epithelial goblet cells deprived of glucose. Am J Physiol Gastrointest Liver Physiol 2004; 287: G1168–G1174.PubMedCrossRefGoogle Scholar
  63. 63.
    Barcelo A, Claustre J, Moro F et al. Mucin secretion is modulated by luminal factors in the isolated vascularly perfused rat colon. Gut 2000; 46: 218–224.PubMedCrossRefGoogle Scholar
  64. 64.
    Zopf D, Roth S. Oligosaccharide anti-infective agents. Lancet 1996; 347: 1017–1021.PubMedCrossRefGoogle Scholar
  65. 65.
    Boyle EC, Finlay BB. Bacterial pathogenesis: exploiting cellular adherence. Curr Opin Cell Biol 2003; 15: 633–639.PubMedCrossRefGoogle Scholar
  66. 66.
    Pool-Zobel BL. Lactobacillus and Bifidobacterium mediated antigenotoxicity in the colon of rats. Nutr Cancer 1996; 26: 365–380.PubMedCrossRefGoogle Scholar
  67. 67.
    Rowland IR. Gut microflora and cancer. In: Leeds AR, Rowland IR eds. Gut Flora and Health—Past, Present and Future. London: The Royal Society of Medicine Press Ltd, 1996: 19–25.Google Scholar
  68. 68.
    Tzortzis G, Goulas AK, Gee JM et al. A novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo. J Nutr 2005; 135:1726–1731.PubMedGoogle Scholar
  69. 69.
    Ross GD, Vetvicka V. CR3 (CD11b, CD18): a phagocyte and NK cell membrane receptor with multiple ligand specificities and functions. Clin Exp Immunol 1993; 92: 181–184.PubMedGoogle Scholar
  70. 70.
    Brown GD, Gordon S. Immune recognition. A new receptor for β-glucans. Nature 2001; 413:36–37.PubMedCrossRefGoogle Scholar
  71. 71.
    Murosak S, Muroyama K, Yamamoto Y et al. Nigerooligosaccharides augments natural killer activity of hepatic mononuclear cells in mice. Int Immunopharmacol 2002; 2: 151–159.PubMedCrossRefGoogle Scholar
  72. 72.
    Schley PD, Field CJ. The immune-enhancing effects of dietary fibers and prebiotics. Br J Nutr 2002; 87 (suppl):S221–S230.PubMedGoogle Scholar
  73. 73.
    Erickson KL, Medina EA, Hubbard NE. Micronutrients and innate immunity. J Infect Dis 2000; 182:S5–S10.PubMedCrossRefGoogle Scholar
  74. 74.
    López-Varela S, González-Gross M, Marcos A. Functional foods and the immune system: a review. Eur J Clin Nutr 2002; 56(suppl 3): S29–S33.PubMedCrossRefGoogle Scholar
  75. 75.
    Meydani M. Effect of functional food ingredients: vitamin E modulation of cardiovascular disease and immune status in the elderly. Am J Clin Nutr 2000; 71 (suppl):1665S–1668S.PubMedGoogle Scholar
  76. 76.
    Weidermann U, Hanson LA, Bremell T et al. Increased translocation of Escherichia coli and development of arthritis in vitamin A-deficient rats. Infect Immun 1995; 63: 3062–3068.Google Scholar
  77. 77.
    Domeneghini C, Di Giancamillo A, Arrighi S et al. Gut-trophic feed additives and their effects upon the gut structure and intestinal metabolism. State of the art in the pig and perspectives towards humans. Histol Histopathol 2006; 21:273–283.PubMedGoogle Scholar
  78. 78.
    Holick MF. Vitamin D: its role in cancer prevention and treatment. Prog Biophys Mol Biol 2006; doi:10.1016/j.pbiomolbio.2006.02.014.Google Scholar
  79. 79.
    Froicu M, Zhu Y, Cantorna MT. Vitamin D receptor is required to control gastrointestinal immunity in IL-10 knockout mice. Immunology 2006; 117:310–318.PubMedCrossRefGoogle Scholar
  80. 80.
    Lin R, White JH. The pleiotropic actions of vitamin D. BioEssays 2003; 26:21–28.CrossRefGoogle Scholar
  81. 81.
    Yu V. Scientific rationale and benefits of nucleotide supplementation of infant formula. J Paediatr Child Health 2002; 38:543–549.PubMedCrossRefGoogle Scholar
  82. 82.
    Craig WJ. Health-promoting properties of common herbs. Am J Clin Nutr 1999; 70(suppl): 491S–499S.PubMedGoogle Scholar
  83. 83.
    Borchers AT, Stern JS, Hackman RM et al. Mushrooms, tumors and immunity. Proc Soc Exp Biol Med 1999; 221:281–293.PubMedCrossRefGoogle Scholar
  84. 84.
    Broome CS, McArdle F, Kyle JAM et al. An increase in selenium intake improves immune function and poliovirus handling in adults with marginal selenium status. Am J Clin Nutr 2004; 80:154–162.PubMedGoogle Scholar

Copyright information

© Landes Bioscience and Springer Science+Business Media 2008

Authors and Affiliations

  • Lesley Hoyles
    • 1
  • Jelena Vulevic
    • 1
  1. 1.Food Microbial Sciences Unit, School of Food BiosciencesThe University of ReadingReadingUK

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