Principles of Targeted Mutagenesis in the Moss Physcomitrella Patens

  • Didier Schaefer
  • Jean-Pierre Zrÿd
Chapter

Abstract

Highly efficient gene targeting is a unique feature of the moss Physcomitrella patens. It is now possible, in a multicellular eukaryote, to use the gene replacement technology which has been so successful in yeast. Careful design of transforming vector is nevertheless a key to successful generation of targeted plants.

Keywords

Selectable Marker LoxP Site Replacement Vector Insertion Vector Flank Genomic Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adair, G.M., Scheerer, J.B,. Brotherman, A., McConville, S., Wilson, J.H., & Nairn, R.S. (1998) Targeted recombination at the Chinese hamster APRT locus using insertion versus replacement vectors. Somatic Cell & Molecular Genetics 24, 91–105Google Scholar
  2. Bouche, N., & Bouchez, D. (2001) Arabidopsis gene knockout: phenotypes wanted. Curr Opin Plant Biol. 4, 111–117Google Scholar
  3. Chakhparonian, M. (2001) Développement d’outils de la mutagenèse ciblée par recombinaison homologue chez Physcomitrella patens. PhD thesis. Université de Lausanne, Switzerland “http://www.unil.ch/lpc/docs/ ”
  4. Cormack, R.S., & Somssich, I.E. (1997) Rapid amplification of genomic ends (RAGE) as a simple method to clone flanking genomic DNA. Gene 194, 273–276PubMedCrossRefGoogle Scholar
  5. Giaever, G., Chu, A.M., Ni, L., Connelly, C., Riles, L., Veronneau, S., Dow, S., Lucau-Danila, A., Anderson, K., Andre, B., Arkin, A.P., Astromoff, A., El-Bakkoury, M., Bangham, R., Benito, R., Brachat, S., Campanaro, S., Curtis, M., Davis, K., Deutschbauer, A., Entian, K.D., Flaherty, P., Foury, F., Garfinkel, D.J., Gerstein, M., Gotte, D., Guldener, U., Hegemann, J.H., Hempel, S., Herman, Z., Jaramillo, D.F., Kelly, D.E., Kelly, S.L., Kotter, P., LaBonte, D., Lamb, D.C., Lan, N., Liang, H., Liao, H., Liu, L., Luo, C., Lussier, M., Mao, R., Menard, P., Ooi, S.L., Revuelta, J.L., Roberts, C.J., Rose, M., Ross-Macdonald, P., Scherens, B., Schimmack, G., Shafer, B., Shoemaker, D.D., Sookhai-Mahadeo, S., Storms, R.K., Strathern, J.N., Valle, G., Voet, M., Volckaert, G., Wang, C.Y., Ward, T.R., Wilhelmy, J., Winzeler, E.A., Yang, Y., Yen, G., Youngman, E., Yu, K., Bussey, H., Boeke, J.D., Snyder, M., Philippsen, P., Davis, R.W., & Johnston, M. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418, 387–391PubMedCrossRefGoogle Scholar
  6. Hohe, A., & Reski, R. (2003) A tool for understanding homologous recombination in plants. Plant Cell Rep. 21, 1135–1142PubMedCrossRefGoogle Scholar
  7. Holtorf, H., Guitton, M.C., & Reski, R. (2002) Plant functional genomics. Naturwissenschaften 89, 235–249PubMedCrossRefGoogle Scholar
  8. Jeon, J.S., Lee, S., Jung, K.H., Jun, S.H., Jeong, D.H., Lee, J., Kim, C., Jang, S., Yang, K., Nam, J., An, K., Han, M.J., Sung, R.J., Choi, H.S., Yu, J.H., Choi, J.H., Cho, S.Y., Cha, S.S., Kim, S.I., & An, G. (2000) T-DNA insertional mutagenesis for functional genomics in rice. Plant Journal 22, 561–570PubMedCrossRefGoogle Scholar
  9. Kammerer, W., & Cove, D.J. (1996) Genetic analysis of the result of re-transformation of transgenic lines of the moss, Physcomitrella patens. Mol. Gen. Genet. 250, 380–382PubMedGoogle Scholar
  10. Mengiste, T., & Paszkowski, J. (1999) Prospects for the precise engineering of plant genomes by homologous recombination [Review]. Biol. Chem. 380, 749–758PubMedCrossRefGoogle Scholar
  11. Müller, U. (1999) Ten years of gene targeting: targeted mouse mutants, from vector design to phenotype analysis [Review]. Mech. Devel. 82, 3–21CrossRefGoogle Scholar
  12. Nishiyama, T., Fujita, T., Shin, I.T., Seki, M., Nishide, H., Uchiyama, I., Kamiya, A., Carninci, P., Hayashizaki, Y., Shinozaki, K., Kohara, Y., & Hasebe, M. (2003) Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: implication for land plant evolution. Proc. Natl. Acad. Sci. U. S. A. 100, 8007–8012PubMedCrossRefGoogle Scholar
  13. Ow, D.W. (2002) Recombinase-directed plant transformation for the post genomic era. Plant Mol. Biology 48, 183–200CrossRefGoogle Scholar
  14. Paques, F., & Haber, J.E. (1999) Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae [Review]. Micro. & Mol. Biol. Reviews. 63, 349–404Google Scholar
  15. Parinov, S., & Sundaresan, V. (2000) Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project [Review]. Curr. Opin. Biotech. 11, 157–161PubMedCrossRefGoogle Scholar
  16. Puchta, H. (1998) Towards targeted transformation in plants. Trends in Plant Science 3, 77–78Google Scholar
  17. Puchta, H. (2002) Gene replacement by homologous recombination in plants. Plant Mol. Biol. 48, 173–182PubMedCrossRefGoogle Scholar
  18. Rensing, S.A., Rombauts, S., Van de Peer, Y., & Reski, R. (2002) Moss transcriptome and beyond. Trends in Plant Science 7, 535–538PubMedCrossRefGoogle Scholar
  19. Rothstein, R. (1991) Targeting, disruption, replacement and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 194, 281–301Google Scholar
  20. Sakakibara, K., Nishiyama, T., Sumikawa, N., Kofuji, R., Murata, T., & Hasebe, M. (2003) Involvement of auxin and a homeodomain-leucine zipper I gene in rhizoid development of the moss Physcomitrella patens. Development 130, 4835–4846PubMedCrossRefGoogle Scholar
  21. Sauer, B. (1993) Manipulation of the transgene by site-specific recombination: use of cre recombinase. Methods Enzymol. 225, 890–900PubMedCrossRefGoogle Scholar
  22. Schaefer, D.G. (1994) Molecular genetic approaches to the biology of the moss Physcomitrella patens. PhD Thesis. University of Lausanne, Switzerland “http://www.unil.ch/lpc/docs/DSThesis.htm”
  23. Schaefer, D.G. (200 1) Gene targeting in Physcomitrella patens. Curr. Opin. Plant Biol. 4, 143–150Google Scholar
  24. Schaefer, D.G. (2002) A new moss genetics: targeted mutagenesis in Physcomitrella patens. Annu Rev Plant Biol 53, 477–501PubMedCrossRefGoogle Scholar
  25. Schaefer, D.G., & Zryd, J-P. (1997) Efficient gene targeting in the moss Physcomitrella patens. Plant J. 11, 1195–1206Google Scholar
  26. Schaefer, D.G., & Zrÿd, J-P. (2001) The moss Physcomitrella patens now and then. Plant Phys. 127, 1430–1438CrossRefGoogle Scholar
  27. Schaefer, D.G., Zrÿd, J-P., Knight, C.D., & Cove, D.J. (1991) Stable transformation of the moss Physcomitrella patens. Mol. Gen. Genet. 226, 418–424Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2004

Authors and Affiliations

  • Didier Schaefer
    • 1
  • Jean-Pierre Zrÿd
    • 1
  1. 1.Laboratory of Plant Cell Genetics, Department of Plant Molecular BiologyUniversity of LausanneLausanneSwitzerland

Personalised recommendations