Activity-Based Protein Profiling at the Host–Pathogen Interface
- 1.4k Downloads
Activity-based protein profiling (ABPP) is a technique for selectively detecting reactive amino acids in complex proteomes with the aid of chemical probes. Using probes that target catalytically active enzymes, ABPP can rapidly define the functional proteome of a biological system. In recent years, this approach has been increasingly applied to globally profile enzymes active at the host–pathogen interface of microbial infections. From in vitro co-culture systems to animal models of infection, these studies have revealed enzyme-mediated mechanisms of microbial pathogenicity, host immunity, and metabolic adaptation that dynamically shape pathogen interactions with the host.
We gratefully acknowledge Pamela Chang, Joshua Gendron, Yannick Jacob, and Lindsay Triplett for providing helpful feedback on this manuscript. Y. K. was supported by an NIH Predoctoral Training Grant (T32GM067543).
- Bender KO, Garland M, Ferreyra JA, Hryckowian AJ, Child MA, Puri AW, Solow-Cordero DE, Higginbottom SK, Segal E, Banaei N et al (2015) A small-molecule antivirulence agent for treating Clostridium difficile infection. Sci Transl Med 7:306ra148Google Scholar
- Lentz CS, Ordonez AA, Kasperkiewicz P, La Greca F, O’Donoghue AJ, Schulze CJ, Powers JC, Craik CS, Drag M, Jain SK et al (2016) Design of selective substrates and activity-based probes for Hydrolase Important for Pathogenesis 1 (HIP1) from Mycobacterium tuberculosis. ACS Infect Dis 2:807–815CrossRefGoogle Scholar
- Misas-Villamil JC, van der Burgh AM, Grosse-Holz F, Bach-Pages M, Kovacs J, Kaschani F, Schilasky S, Emon AE, Ruben M, Kaiser M et al (2017) Subunit-selective proteasome activity profiling uncovers uncoupled proteasome subunit activities during bacterial infections. Plant J 90:418–430CrossRefGoogle Scholar
- Wiedner SD, Burnum KE, Pederson LM, Anderson LN, Fortuin S, Chauvigne- Hines LM, Shukla AK, Ansong C, Panisko EA, Smith RD et al (2012) Multiplexed activity-based protein profiling of the human pathogen Aspergillus fumigatus reveals large functional changes upon exposure to human serum. J Biol Chem 287:33447–33459CrossRefGoogle Scholar