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Threading Microarrays into Novel Applications

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Heat Shock Proteins in Human Diseases

Part of the book series: Heat Shock Proteins ((HESP,volume 21))

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Abstract

Introduction HSP90 is known as a stabilizer of the proteome and is required for many newly synthesized proteins and introducing damaged proteins back into the refolding chaperone cycle. Due to its key position and interaction with several hundreds of proteins in a cell, it is a target for noxious cells and a responsive sensitive biomarker for cellular stress. Cellular stress is when unfolded proteins are formed by, e.g. mutational events, changes in the osmolality, or redox status. One challenge is to monitor and discriminate the cellular answers to relevant and reliable signals. The aim of this narrative review is to provide an overview of currently available microarray applications using HSP90 as the target.

Methods Pubmed search was performed for available studies on microarray-oriented techniques.

Results Different strategies have been used to measure the presence of HSP90, e.g., immunologically, by turnover, or binding activities. Protein microarrays have a broad application range. As a highly miniaturized assay system they are used to study protein-ligand or protein-protein interaction and used to measure the presence of a target as a diagnostic tool. Q-Dots have interesting electrical and optical properties. The exploitation of its optoelectronic properties for sensing plays a momentous role in the biomolecular diagnostic assay for HSP90.

Conclusions Target-oriented trawling of natural product compound libraries can help to identify novel compounds that inactivate the HSP90 function or increase the affinity for the natural ligand or client or help to restore the activity. HSP90 is a biomarker used in future as a stress marker for disease treatments, observing endurance status of athlete or life style management.

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Abbreviations

17-AAG:

17-N-allylamino-17-demethoxygeldanamycin

Aarsd1:

muscle specific cochaperone (alanyl-tRNA synthetase domain-containing 1)

AIF:

apopotosis inducing factor

Apaf1:

apoptotic protease activating factor 1

CdSe:

cadmium selenide

CgA:

chromogranin A

CTD:

c-terminal domain

Cy5:

Cyanin 5

IgA:

Immunoglobulin A

MD:

middle domain

NTD:

N-terminal domain

PMA:

protein microarray

PPI:

protein-protein interaction

PU-H71:

6-amino-8-[(6-iodo-1,3-benzodioxol-5-yl)thio]-N-(1-methylethyl)-9H-purine-9-propanamine

Q-dots:

quantum dots

SPR:

surface plasma resonance

ZnS:

zinc sulfide

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Acknowlegements

Funding: AK financed by Exposè scholarship from the Graduate Academy Leibniz University Hannover.

Disclosure of Interests

All authors declare they have no conflict of interest.

Ethical Approval for Studies Involving Human

This article does not contain any studies with human participants performed by any of the authors.

Ethical Approval for Studies Involving Animals

This article does not contain any studies with animals performed by any of the authors.

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Authors and Affiliations

  1. Gottfried-Wilhelm-Leibniz University of Hannover BMWZ (Zentrum für Biomolekulare Wirkstoffe), Hannover, Germany

    Anusha Kishore & Carsten Zeilinger

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  1. Anusha Kishore
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  2. Carsten Zeilinger
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Correspondence to Carsten Zeilinger .

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Editors and Affiliations

  1. Department of Medicine and Precision Therapeutics Proteogenomics Diagnostic Center, Eleanor N. Dana Cancer Center University of Toledo College of Medicine and Life Sciences, Toledo, OH, USA

    Alexzander A. A. Asea

  2. Department of Medicine and Precision Therapeutics Proteogenomics Diagnostic Center, Eleanor N. Dana Cancer Center University of Toledo College of Medicine and Life Sciences, Toledo, OH, USA

    Punit Kaur

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Kishore, A., Zeilinger, C. (2020). Threading Microarrays into Novel Applications. In: Asea, A.A.A., Kaur, P. (eds) Heat Shock Proteins in Human Diseases. Heat Shock Proteins, vol 21. Springer, Cham. https://doi.org/10.1007/7515_2020_7

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