pp 1-17 | Cite as

Fluorescent Probes for Diagnostics of β-Galactosidase: From Micro to Macro

  • Anyao Bi
  • Xinchen Jiang
  • Tang Gao
  • Shuqi Yang
  • Yi Liu
  • Xiaohui Liu
  • Wenbin Zeng
Part of the Topics in Medicinal Chemistry book series


β-Galactosidase (β-gal) is an enzyme commonly served as a reporter for the examination of transcription and transfection efficiencies. Due to its overexpression in primary and metastatic ovarian cancers, β-gal was also usually regarded as a molecular target for visualizing peritoneal metastases from ovarian cancers. Moreover, β-gal has been studied as a potential therapeutic target for lactose intolerance via gene replacement therapy in recent years. Interestingly, there were some reports that β-gal has been abnormally accumulated in senescent cells, which allowing this senescence-associated β-gal to be a significant biomarker for senescence. The great significance of β-gal has attracted many researchers’ attentions in developing highly selective and sensitive approaches to monitor the activity of this enzyme in vitro and in vivo. In this review, we reported the recent development of the various materials for β-gal detection and their application in disease progression monitoring, with a focus on fluorescent probe, nanomaterials, and biomolecules. Finally, the trends for the further development of the probe for fluorescence-guided diagnosis in clinical cases and its preclinical potential value were proposed.


β-Galactosidase AIE Fluorescence Nanomaterials Tumor 



We are grateful for the financial supports from the National Natural Science Foundation of China (81971678, 81671756), Key Research Project of Science and Technology Foundation of Hunan Province (2017SK2093 and 2019SK2211), Key Research Project of Science and Technology Foundation of Changsha (kq1801063), and Fundamental Research Funds for the Central Universities of Central South University (2018zzts041, 2018dcyj067).

Compliance with Ethical Standards

Conflict of Interest: There are no conflicts to declare.

Ethical Approval: There is no ethical approval to declare.

Informed Consent: There is no informed consent to declare.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Anyao Bi
    • 1
    • 2
  • Xinchen Jiang
    • 1
    • 2
  • Tang Gao
    • 1
    • 2
  • Shuqi Yang
    • 1
    • 2
  • Yi Liu
    • 1
    • 2
  • Xiaohui Liu
    • 1
    • 2
  • Wenbin Zeng
    • 1
    • 2
  1. 1.Xiangya School of Pharmaceutical Sciences, Central South UniversityChangshaChina
  2. 2.Molecular Imaging Research Center, Central South UniversityChangshaChina

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