Nitric Oxide in Plant Growth, Development and Stress Physiology pp 269-280

Part of the Plant Cell Monographs book series (CELLMONO, volume 5)

Fluorometric Detection of Nitric Oxide with Diaminofluoresceins (DAFs): Applications and Limitations for Plant NO Research

  • Nao O. Arita
  • Michael F. Cohen
  • Gaku Tokuda
  • Hideo Yamasaki
Chapter

Abstract

Nitric oxide (NO), a reactive nitrogen species, serves as a signaling molecule in plants, animals, fungi, and bacteria. In spite of its potential significance, however, the unique challenges of NO research can bring confusion to investigations, primarily due to difficulties in detecting and quantifying biological NO production. To overcome such barriers, we recommend that researchers choose a combinatorial approach for monitoring NO levels, in which multiple methods having distinct detection principles are employed. After an overview of the major methodologies for NO detection, we highlight the usefulness and application limits of the fluorescence probe diaminofluorescein (DAF), which has been preferentially applied in studies of plant NO-producing systems.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • Nao O. Arita
    • 1
  • Michael F. Cohen
    • 2
  • Gaku Tokuda
    • 3
  • Hideo Yamasaki
    • 1
  1. 1.Faculty of ScienceUniversity of the RyukyusOkinawaJapan
  2. 2.Department of BiologySonoma State UniversityRohnert ParkUSA
  3. 3.Center of Molecular Biosciences (COMB)University of the RyukyusOkinawaJapan

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