Fluorometric Detection of Nitric Oxide with Diaminofluoresceins (DAFs): Applications and Limitations for Plant NO Research
Nitric oxide (NO), a reactive nitrogen species, serves as a signaling molecule in plants, animals, fungi, and bacteria. In spite of its potential significance, however, the unique challenges of NO research can bring confusion to investigations, primarily due to difficulties in detecting and quantifying biological NO production. To overcome such barriers, we recommend that researchers choose a combinatorial approach for monitoring NO levels, in which multiple methods having distinct detection principles are employed. After an overview of the major methodologies for NO detection, we highlight the usefulness and application limits of the fluorescence probe diaminofluorescein (DAF), which has been preferentially applied in studies of plant NO-producing systems.
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