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Embryogenesis in Catharanthus roseus: Roles of Some External Factors in Proliferation, Maturation and Germination of Embryos

  • A. Junaid
  • A. Mujib
  • M. A. Bhat
  • A. Ilah
  • M. P. Sharma
Chapter
Part of the Plant Cell Monographs book series (CELLMONO, volume 2)

Abstract

Catharanthus roseus is an important medicinal plant that contains two well-known anticancerous alkaloids, vincristine and vinblastine. Cell culture technology has been employed for a long time to improve the alkaloid yield. In this chapter, various processes of somatic embryogenesis such as embryo induction, proliferation, maturation and germination are described. In this embryogenic system, embryos showed irregularities in structure and registered poor conversion frequency. Several carbon sources were added in order to improve the embryo quality before germination: 3% fructose or 3–6% maltose were found to be effective during maturation. Plantlet conversion was high on 3–6% maltose and 3% fructose. In addition, suspension culture, indirect embryogenesis and loss of embryogenic potential with time are discussed in brief. The authors felt that the low yields of vincristine and vinblastine may be improved if the single cell embryo origin concept is utilized in a genetic modification program.

Keywords

Somatic Embryo Somatic Embryogenesis Embryogenic Callus Catharanthus Roseus Somatic Embryo Maturation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Authors and Affiliations

  • A. Junaid
    • 1
  • A. Mujib
    • 1
  • M. A. Bhat
    • 1
  • A. Ilah
    • 1
  • M. P. Sharma
    • 1
  1. 1.Cellular Differentiation and Molecular Genetics Section, Department of BotanyHamdard UniversityNew DelhiIndia

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