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Adipose-Derived Mesenchymal Stem Cells Promote Growth and Migration of Lung Adenocarcinoma Cancer Cells

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Cancer Biology and Advances in Treatment

Part of the book series: Advances in Experimental Medicine and Biology ((ICRRM,volume 1292))

Abstract

Introduction: Mesenchymal stem cells (MSCs) have been used in cancer therapy as vehicles to deliver therapeutic materials such as drugs, apoptosis inducers and cytokines due to their ability to migrate and home at the tumour site. Furthermore, MSCs have been genetically engineered to produce anticancer molecules such as TRAIL that can induce apoptosis of cancer cells. However, MSCs’ presence in the tumour microenvironment has shown to be involved in promoting tumour growth and progression. Therefore, the roles of MSCs either promoting or suppressing tumorigenesis need to be investigated. Methods: Human adipose-derived MSCs (Ad-MSCs) and A549 cells are co-cultured together in indirect co-culture system using Transwell insert. Following co-culture, both cells were analysed in terms of growth rate, migration ability, apoptosis and gene expression for genes involved in migration and stemness characteristics. Results: The result shows that Ad-MSCs promoted the growth of A549 cells when indirectly co-cultured for 48 and 72 h. Furthermore, Ad-MSCs significantly enhanced the migration rate of A549 cells. The increased in migration rate was in parallel with the significant increase of MMP9. There are no significant changes observed in the expression of TWIST2, CDH2 and CDH1, genes involved in the epithelial-to-mesenchymal transition (EMT). Ad-MSCs also protect A549 cancer cells from undergoing apoptosis and increase the survival of cancer cells. Conclusion: Secretion of soluble factors from Ad-MSCs has been shown to promote the growth and metastatic characteristics of A549 cancer cells. Therefore, the use of Ad-MSCs in cancer therapy needs to be carefully evaluated in the long-term aspect.

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Abbreviations

AD:

Adenocarcinoma

Ad-MSCs:

Human adipose-derived mesenchymal stem cells

BM-MSCs:

Bone marrow-derived mesenchymal stem cells

cDNA:

Complementary DNA

CSCs:

Cancer stem cells

EMT:

Epithelial-to-mesenchymal transition

LCC:

Large-cell carcinoma

MSCs:

Mesenchymal stem cells

NSCLC:

Non-small cell lung carcinoma

SCC:

Squamous cell carcinoma

TME:

Tumour microenvironment

TRAIL:

TNF-related apoptosis-inducing ligand

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Acknowledgements

This study was supported by grants from Universiti Sains Malaysia (USM) Research University Grant (1001/CIPPT/8012203).

Conflict of Interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Authors’ Contributions

NZ contributed in design; performed the experiment; collected, analysed and interpreted the experimental data; and wrote the manuscript. BHY contributed in design and analysed the experimental data and was involved in the writing and editing of the manuscript. Both authors read and approved the final manuscript.

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Authors and Affiliations

  1. Regenerative Medicine Cluster, Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Kepala Batas, Penang, Malaysia

    Norashikin Zakaria & Badrul Hisham Yahaya

Authors
  1. Norashikin Zakaria
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  2. Badrul Hisham Yahaya
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Corresponding author

Correspondence to Badrul Hisham Yahaya .

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Editors and Affiliations

  1. Stem Cell Institute, University of Science, Vietnam National University, Ho Chi Minh, Vietnam

    Phuc Van Pham

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Zakaria, N., Yahaya, B.H. (2020). Adipose-Derived Mesenchymal Stem Cells Promote Growth and Migration of Lung Adenocarcinoma Cancer Cells. In: Pham, P.V. (eds) Cancer Biology and Advances in Treatment. Advances in Experimental Medicine and Biology(), vol 1292. Springer, Cham. https://doi.org/10.1007/5584_2019_464

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