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Induced Pluripotent Stem Cells for Regenerative Medicine: Quality Control Based on Evaluation of Lipid Composition

  • Yusuke Nakamura
  • Yasuo ShimizuEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1212)

Abstract

Clinical application of induced pluripotent stem cells (iPSCs), which can be differentiated into a wide variety of functional cells, is underway and some clinical trials have already been performed or are ongoing. On the other hand, the risk of carcinogenesis is an issue and the mechanism of cellular reprograming remains unknown. When iPSCs and differentiated cells are used for medical applications, quality control is also important. Here we discuss the possibility of performing quality control of iPSCs by evaluation of phospholipids, which are not just structural components of lipid bilayer membranes, but also have multiple physiological functions. Recently, methods for analysis of lipids have become more widely available and easier to perform. This article reviews the role of iPSCs in regenerative medicine and examines the possibility of using phospholipids for quality control of iPSCs and differentiated cells.

Keywords

Induced pluripotent stem cells Regenerative medicine Lipids Quality control LC-MS Imaging mass spectrometry 

Abbreviations

ALS

amyotrophic lateral sclerosis

FIH

first-in-human

FOP

Fibrodysplasia ossificans progressive

IMS

imaging mass spectrometry

iPSCs

induced pluripotent stem cells

KLF4

Kruppel like factor 4

LC-MS

liquid chromatography-mass spectrometry

pPE

plasmalogen phosphatidylethanolamine

SM

sphingomyelin

SOX2

SRY-box 2

Notes

Acknowledgements

This work was supported by a Japan Society for the Promotion of Science (JSPS) KAKENHI Grant, Japan to Y.S (16 K09553).

Author Contributions

Y.N. wrote the manuscript. Y.S. designed and wrote the manuscript. Both authors reviewed the manuscript.

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Pulmonary Medicine and Clinical ImmunologyDokkyo Medical University School of MedicineMibu, TochigiJapan

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