Effects of Glutathione on Hydrolytic Enzyme Activity in the Mouse Hepatocytes

  • Iwona Stanisławska
  • Bożena Witek
  • Marek ŁypEmail author
  • Danuta Rochon-Szmejchel
  • Adam Wróbel
  • Wojciech Fronczyk
  • Agnieszka Kamińska
  • Adam Kołątaj
  • Daniel Załuski
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1116)


In this study, the effect of glutathione (GSH) on the activity of hydrolytic enzymes of lysosomal, microsomal, and cytosolic fractions was studied in the mouse hepatocytes. The experiments involved 30 Swiss male mice, divided into the experimental and control groups of 15 mice each. The former group received injections of 12 μL/g of GSH solution at a dose of 100 μg/g body weight, whereas the latter received 12 μL/g of physiological saline, all given intraperitoneally daily for 7 days. Then, fragments of liver tissue were collected from the euthanized animals and processed to obtain lysosomal, microsomal, and cytosolic fractions of hepatocytes. The activity of the following enzymes was investigated in vitro: β-glucuronidase, β-galactosidase, β-glucosidase, N-acetyl-hexosaminidase, lysosomal esterase and lipase, acid phosphatase, cathepsin D and L, leucine aminopeptidase, and alanine aminopeptidase. We found that GSH, administered in vivo in the mouse, in the main (73% cases), increased the in vitro activity of the majority enzymes abovementioned, although the effect was somehow variable, depending on the fraction of hepatocytes and the type of enzyme. The findings imply that GSH supplementation may intensify the rate of cellular hydrolytic degradation, i.e., the rate of disposal by the cell of unwanted materials.


Cellular fraction Glutathione Hepatocytes Hydrolytic enzymes Mouse liver 


Conflicts of Interest

The authors declare no conflict of interest in relation to this article.

Ethical Approval

All procedures performed in this study were in accordance with the ethical standards of the institution at which the studies were conducted and with the 1964 Helsinki Declaration and its later amendments. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The experiments were carried out with the approval of the Bioethics Committee of the Świętokrzyska Chamber of Physicians in the city of Kielce in Poland (permit no. 49/2016).


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Iwona Stanisławska
    • 1
  • Bożena Witek
    • 2
  • Marek Łyp
    • 1
    Email author
  • Danuta Rochon-Szmejchel
    • 3
  • Adam Wróbel
    • 4
  • Wojciech Fronczyk
    • 5
  • Agnieszka Kamińska
    • 6
  • Adam Kołątaj
    • 1
  • Daniel Załuski
    • 7
  1. 1.Department of DieteticsCollege of RehabilitationWarsawPoland
  2. 2.Department of Animal PhysiologyThe Jan Kochanowski UniversityKielcePoland
  3. 3.“Dandiete” Diet Outpatient ClinicNowe Miasto LubawskiePoland
  4. 4.“Expertdent”KielcePoland
  5. 5.Institute of Genetics and Animal BreedingPolish Academy of SciencesMagdalenkaPoland
  6. 6.The Cardinal Stefan Wyszyński UniversityWarsawPoland
  7. 7.Department of PharmacognosyThe Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus UniversityBydgoszczPoland

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