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Novel Model of Somatosensory Nerve Transfer in the Rat

  • Adriana M. Paskal
  • Wiktor Paskal
  • Kacper Pelka
  • Martyna Podobinska
  • Jaroslaw Andrychowski
  • Pawel K. WlodarskiEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1096)

Abstract

Nerve transfer (neurotization) is a reconstructive procedure in which the distal denervated nerve is joined with a proximal healthy nerve of a less significant function. Neurotization models described to date are limited to avulsed roots or pure motor nerve transfers, neglecting the clinically significant mixed nerve transfer. Our aim was to determine whether femoral-to-sciatic nerve transfer could be a feasible model of mixed nerve transfer. Three Sprague Dawley rats were subjected to unilateral femoral-to-sciatic nerve transfer. After 50 days, functional recovery was evaluated with a prick test. At the same time, axonal tracers were injected into each sciatic nerve distally to the lesion site, to determine nerve fibers’ regeneration. In the prick test, the rats retracted their hind limbs after stimulation, although the reaction was moderately weaker on the operated side. Seven days after injection of axonal tracers, dyes were visualized by confocal microscopy in the spinal cord. Innervation of the recipient nerve originated from higher segments of the spinal cord than that on the untreated side. The results imply that the femoral nerve axons, ingrown into the damaged sciatic nerve, reinnervate distal targets with a functional outcome.

Keywords

Axonal tracers Nerve fibers Nerve transfer Neuroregeneration Neurotization Sciatic nerve Spinal cord injury 

Notes

Acknowledgments

Supported by the Medical University of Warsaw, Second Faculty of Medicine, Grant No. 2 W8/NM2/16, and the statutory funds from the First Faculty of Medicine, Medical University of Warsaw. The funder had no involvement in the study design nor in data collection, analysis, or interpretation.

Conflicts of Interest

The authors declare no conflicts of interest in relation to this article.

Supplementary material

Video 1

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References

  1. Cao X, Li J, Cao Y, Cai J (2003) C3, 4 transfer for neurotization of C5, 6 nerve roots in brachial plexus injury in a rabbit model. J Reconstr Microsurg 19(4):265–270CrossRefPubMedGoogle Scholar
  2. Gomez-Amaya SM, Barbe MF, de Groat WC, Brown JM, Tuite GF, Corcos J, Fecho SB, Braverman AS, Ruggieri MR Sr (2015) Neural reconstruction methods of restoring bladder function. Nat Rev Urol 12(2):100–118CrossRefPubMedPubMedCentralGoogle Scholar
  3. Isla A, Martinez JR, Perez Lopez C, Perez Conde C, Morales C, Avendano C (2006) Anatomical and functional connectivity of the transected ulnar nerve after accessory nerve neurotization in the cat. J Neurosurg Sci 50(2):33–40PubMedGoogle Scholar
  4. Krieger LM, Krieger AJ (2000) The intercostal to phrenic nerve transfer: an effective means of reanimating the diaphragm in patients with high cervical spine injury. Plast Reconstr Surg 105(4):1255–1261PubMedGoogle Scholar
  5. Louie G, Mackinnon SE, Dellon AL, Patterson GA, Hunter DA (1987) Medial antebrachial cutaneous--lateral femoral cutaneous neurotization in restoration of sensation to pressure-bearing areas in a paraplegic: a four-year follow-up. Ann Plast Surg 19(6):572–576CrossRefPubMedGoogle Scholar
  6. Noordin S, Ahmed M, Rehman R, Ahmad T, Hashmi P (2008) Neuronal regeneration in denervated muscle following sensory and muscular neurotization. Acta Orthop 79(1):126–133CrossRefPubMedGoogle Scholar
  7. Rodriguez A, Chuang DC, Chen KT, Chen RF, Lyu RK, Ko YS (2011) Comparative study of single-, double-, and triple-nerve transfer to a common target: experimental study of rat brachial plexus. Plast Reconstr Surg 127(3):1155–1162CrossRefPubMedGoogle Scholar
  8. Senjaya F, Midha R (2013) Nerve transfer strategies for spinal cord injury. World Neurosurg 80(6):e319–e326CrossRefPubMedGoogle Scholar
  9. Song J, Chen L, Gu Y (2010) Effect of ipsilateral C7 nerve root transfer on restoration of rat upper trunk muscle and nerve function after brachial plexus root avulsion. Orthopedics 33(12):886PubMedGoogle Scholar
  10. Spyropoulou GA, Lykoudis EG, Batistatou A, Papalois AE, Tagaris G, Pikoulis E, Bastounis E, Papadopoulos O (2007) New pure motor nerve experimental model for the comparative study between end-to-end and end-to-side neurorrhaphy in free muscle flap neurotization. J Reconstr Microsurg 23(7):391–398CrossRefPubMedGoogle Scholar
  11. Vialle R, Lacroix C, Harding I, Loureiro MC, Tadie M (2010) Motor and sensitive axonal regrowth after multiple intercosto-lumbar neurotizations in a sheep model. Spinal Cord 48(5):367–374CrossRefPubMedGoogle Scholar
  12. Viterbo F, Ripari WT (2008) Nerve grafts prevent paraplegic pressure ulcers. J Reconstr Microsurg 24(4):251–253CrossRefPubMedGoogle Scholar
  13. Xiao CG, Du MX, Dai C, Li B, Nitti VW, de Groat WC (2003) An artificial somatic-central nervous system-autonomic reflex pathway for controllable micturition after spinal cord injury: preliminary results in 15 patients. J Urol 170(4 Pt 1):1237–1241Google Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Adriana M. Paskal
    • 1
    • 2
  • Wiktor Paskal
    • 1
    • 2
  • Kacper Pelka
    • 2
  • Martyna Podobinska
    • 1
  • Jaroslaw Andrychowski
    • 3
  • Pawel K. Wlodarski
    • 2
    Email author
  1. 1.Department of Experimental and Clinical Physiology, Laboratory of Center for Preclinical ResearchWarsaw Medical UniversityWarsawPoland
  2. 2.Department of Histology and Embryology, Laboratory of Center for Preclinical ResearchWarsaw Medical UniversityWarsawPoland
  3. 3.Department of Neurology and Neurosurgery, Faculty of Medical SciencesUniversity of Warmia and Mazury in OlsztynOlsztynPoland

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