Serum Diamine Oxidase in Pseudoallergy in the Pediatric Population

  • Joanna Kacik
  • Barbara Wróblewska
  • Sławomir Lewicki
  • Robert Zdanowski
  • Bolesław Kalicki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1039)


Histamine intolerance (pseudoallergy) is a poorly investigated type of food hypersensitivity. The main enzyme responsible for histamine degradation in the extracellular matrix is diamine oxidase (DAO). Disturbances in the concentration or activity of DAO may lead to the development of clinical signs of allergy. The aim of the present work was to assess the DAO concentration, peripheral blood morphology, lymphocytes phenotyping (CD3+, CD4+, CD8+, CD19+, NK cells, NKT cells, and activated T-cells), and natural regulatory Treg (nTregs) cell population (CD4+, CD25+, CD127low, and FoxP3) in 34 pediatric patients with histamine-dependent syndromes. Patients were divided into two groups: classical allergy and pseudoallergy on the basis of IgE concentration. The investigation was based on the analysis of peripheral blood samples. A significantly lower serum DAO, both total and specific IgE, concentration was found in the pseudoallergy group compared with the allergy group. There were no significant differences in blood morphology or lymphocyte populations. A similar level of nTreg lymphocytes was also found in both groups, although it was lower than that present in healthy individuals. The findings suggest that the serum DAO is responsible for the symptoms of histamine intolerance. Moreover, a general decrease in nTreg cells in comparison with healthy individuals may lead to symptom aggravation.


Allergy Angioedema Dermal lesions Headache Immunological disorders Stomachache 



We thank Mr. Piotr Murawski, Head of ICT Department of the Military Institute of Medicine for assistance in statistical elaboration.

Conflicts of Interest

The authors declare no conflicts of interest in relation to this article.


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Copyright information

© Springer International Publishing AG  2017

Authors and Affiliations

  • Joanna Kacik
    • 1
  • Barbara Wróblewska
    • 2
  • Sławomir Lewicki
    • 3
  • Robert Zdanowski
    • 3
  • Bolesław Kalicki
    • 1
  1. 1.Department of Pediatrics, Pediatric Nephrology and AllergologyMilitary Institute of MedicineWarsawPoland
  2. 2.Department of Immunology and Food MicrobiologyInstitute of Animal Reproduction and Food Research, Polish Academy of SciencesOlsztynPoland
  3. 3.Department of Regenerative Medicine and Cell BiologyMilitary Institute of Hygiene and EpidemiologyWarsawPoland

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