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Comparison of Thymic Stromal Lymphopoietin Concentration in Various Human Biospecimens from Asthma and COPD Patients Measured with Two Different ELISA Kits

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Pathobiology of Pulmonary Disorders

Part of the book series: Advances in Experimental Medicine and Biology ((NR,volume 955))

Abstract

Thymic stromal lymphopoietin (TSLP) seems a promising asthma biomarker. In earlier studies, mainly the serum concentration of TSLP was investigated. The aim of the present study was to compare the TSLP concentration measured by two different ELISA kits in the serum, induced sputum, and exhaled breath condensate in asthma, COPD, and control subjects. The study included 24 asthmatics, 36 patients with COPD, and 12 controls. TSLP concentration was measured with the use of R&D and EIAab commercial ELISA kits. The results obtained with the EIAab kit were 3 to even 45-fold higher than those measured with the R&D kit. Significant differences between the investigated groups were found only for the TSLP concentration in induced sputum. When the R&D kit was used, the highest TSLP levels in induced sputum were found in asthmatics, while the EIAab kit showed the highest TSLP levels in controls. The distribution of results in the Bland-Altman plot was typical for a proportional constant error. TSP concentration in induced sputum might be a more reliable asthma biomarker than serum TSLP. We conclude that TSLP level is highly dependent on the ELISA kit used for the measurement. Thus, judgement on TSLP results obtained with different assays might be confusing and lead to wrong conclusions.

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Conflicts of Interest

The authors declare no conflict of interest related to this article.

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Authors and Affiliations

  1. Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha Street, 02-097, Warsaw, Poland

    Katarzyna Górska, Patrycja Nejman-Gryz, Magdalena Paplińska-Goryca, Małgorzata Proboszcz & Rafał Krenke

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  1. Katarzyna Górska
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  2. Patrycja Nejman-Gryz
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  3. Magdalena Paplińska-Goryca
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  4. Małgorzata Proboszcz
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  5. Rafał Krenke
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Correspondence to Patrycja Nejman-Gryz .

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Editors and Affiliations

  1. Institute of Nursing, Public Higher Medical Professional School in Opole, Opole, Poland

    Mieczyslaw Pokorski

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Górska, K., Nejman-Gryz, P., Paplińska-Goryca, M., Proboszcz, M., Krenke, R. (2016). Comparison of Thymic Stromal Lymphopoietin Concentration in Various Human Biospecimens from Asthma and COPD Patients Measured with Two Different ELISA Kits. In: Pokorski, M. (eds) Pathobiology of Pulmonary Disorders. Advances in Experimental Medicine and Biology(), vol 955. Springer, Cham. https://doi.org/10.1007/5584_2016_162

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