Molecular Interactions in SPR Sensors

  • Josef Štěpánek
  • Hana Vaisocherová
  • Marek Piliarik
Chapter
Association Diffusion Dissociation Equilibrium constant Flow cell Mass transport Rate constants Reaction kinetics Sensor Surface plasmon resonance 

Abbreviations

A

Analyte (free reagent in solution)

B

Complex of analyte and receptor

D

Diffusion coefficient (coefficient of translational diffusion)

Da

Damköhler number

h

Flow cell height

ka

Association rate constant

kd

Dissociation rate constant

K

Equilibrium association constant (binding affinity)

l

Flow cell length

NA

Avogadro's number

PDE

Fundamental equation of analyte transport inside flow cell (partial differential equation)

Pe

Peclét number

R

Receptor (binding target for the analyte immobilized at the sensor surface)

RU

Units of the SPR sensor response (resonance units)

Re

Reynolds number

w

Flow cell width

[X]

Molar concentration of X

x

Space coordinate in direction of the analyte flow

y

Space coordinate in direction perpendicular to the sensor surface

α

Free analyte concentration (≡[A])

α0

Injected analyte concentration

β

Surface concentration of receptor (moles per square area)

γ

Surface concentration of complex (indexed for various types of complexes when necessary)

ξ

Sensor response (in RU)

ξS

Standard sensor response (in RU) corresponding to all receptor sites bound to analyte in 1:1 ratio

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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • Josef Štěpánek
    • 1
  • Hana Vaisocherová
    • 2
  • Marek Piliarik
    • 2
  1. 1.Faculty of Mathematics and PhysicsCharles UniversityPragueCzech Republic
  2. 2.Institute of Radio Engineering and ElectronicsPragueCzech Republic

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