Advertisement

pp 1-31 | Cite as

Instrumentation for Fluorescence Lifetime Measurement Using Photon Counting

  • David J. S. BirchEmail author
  • Graham Hungerford
  • David McLoskey
  • Kulwinder Sagoo
  • Philip Yip
Chapter
Part of the Springer Series on Fluorescence book series

Abstract

We describe the evolution of HORIBA Jobin Yvon IBH Ltd, and its time-correlated single-photon counting (TCSPC) products, from university research beginnings through to its present place as a market leader in fluorescence lifetime spectroscopy. The company philosophy is to ensure leading-edge research capabilities continue to be incorporated into instruments in order to meet the needs of the diverse range of customer applications, which span a multitude of scientific and engineering disciplines. We illustrate some of the range of activities of a scientific instrument company in meeting this goal and highlight by way of an exemplar the performance of the versatile DeltaFlex instrument in measuring fluorescence lifetimes. This includes resolving fluorescence lifetimes down to 5 ps, as frequently observed in energy transfer, nanoparticle metrology with sub-nanometre resolution and measuring a fluorescence lifetime in as little as 60 μs for the study of transient species and kinetics.

Keywords

DeltaFlex Fluorescence lifetime HORIBA Jobin Yvon IBH Photon counting TCSPC 

Notes

Acknowledgement

PY wishes to thank the QuantIC Technology Hub for a research fellowship.

References

  1. 1.
    Lakowicz JR (2006) Principles of fluorescence spectroscopy3rd edn. Springer, New YorkGoogle Scholar
  2. 2.
    Birch DJS, Chen Y, Rolinski OJ (2015) Fluorescence. In: Andrews DL (ed) Photonics. Biological and medical photonics, spectroscopy and microscopy, vol 4. Wiley, Hoboken, p 1Google Scholar
  3. 3.
    Strategic Directions International Inc. (2014) Global assessment report 13th edition and market forecast 2014-18. The laboratory analytical and life science instrumentation industryGoogle Scholar
  4. 4.
    Bollinger LM, Thomas GE (1961) Measurement of the time-dependence of scintillation intensity by a delayed coincidence method. Rev Sci Instrum 32:1044Google Scholar
  5. 5.
    Gaviola Z (1926) Ein Fluorometer, apparat zur messing von Fluoreszenzabklingungszeiten. Z Phys 42:853Google Scholar
  6. 6.
    O’Connor DV, Phillips D (1984) Time-correlated single photon counting. Academic Press, LondonGoogle Scholar
  7. 7.
    Birch DJS, Imhof RE (1991) Time-domain fluorescence spectroscopy using time-correlated single-photon counting. In: Lakowicz JR (ed) Techniques. Topics in fluorescence spectroscopy, vol 1. Plenum, New York, p 1Google Scholar
  8. 8.
    Cundall RB, Dale RE (eds) (1983) Time resolved fluorescence spectroscopy in biochemistry and biology. NATO advanced science institutes series a: life sciences, vol 69. Plenum, New YorkGoogle Scholar
  9. 9.
    Birch DJS (1975) Delayed coincidence fluorescence studies of 9,10-diphenylanthracene and the first four all-trans diphenyl polyenes. PhD thesis, University of ManchesterGoogle Scholar
  10. 10.
    Birch DJS, Imhof RE (1977) A single-photon counting fluorescence decay-time spectrometer. J Phys E Sci Instrum 10:1044Google Scholar
  11. 11.
    Birch DJS, Imhof RE (1981) Coaxial nanosecond flashlamp. Rev Sci Instrum 52:1206Google Scholar
  12. 12.
    Imhof RE, Birch DJS (1982) Kinetic modelling and time resolution in reconvolution analysis. In: Bouchy M (ed) Deconvolution and reconvolution of analytical signals: application to fluorescence spectroscopy. ENSIC-INPL, Nancy, p 411Google Scholar
  13. 13.
    McGuiness CD, Sagoo K, McLoskey D, Birch DJS (2004) A new sub-nanosecond LED at 280 nm: application to protein fluorescence. Meas Sci Technol 15:L19Google Scholar
  14. 14.
    McLoskey D, Campbell D, Allison A, Hungerford G (2011) Fast time-correlated single-photon counting fluorescence lifetime acquisition using a 100 MHz semiconductor excitation source. Meas Sci Technol 22:067001Google Scholar
  15. 15.
    Birch DJS, Imhof RE, Dutch A (1984) Pulse fluorometry using simultaneous acquisition of fluorescence and excitation. Rev Sci Instrum 55:1255Google Scholar
  16. 16.
    Birch DJS, Holmes AS, Gilchrist JR, Imhof RE, Al-Alawi SM, Nadolski BZ (1987) A Multiplexed single photon instrument for routine measurement of time-resolved fluorescence anisotropy. J Phys E Sci Instrum 20:471Google Scholar
  17. 17.
    Birch DJS, Holmes AS, Imhof RE, Nadolski BZ, Suhling K (1988) Multiplexed array fluorometry. J Phys E Sci Instrum 21:415Google Scholar
  18. 18.
    McLoskey D, Birch DJS, Sanderson A, Suhling K, Welch E, Hicks PJ (1996) Multiplexed single-photon counting 1: a time-correlated fluorescence lifetime camera. Rev Sci Instrum 67:2228Google Scholar
  19. 19.
    Suhling K, McLoskey D, Birch DJS (1996) Multiplexed single-photon counting 2: the statistical theory of time-correlated measurements. Rev Sci Instrum 67:2238Google Scholar
  20. 20.
    McLoskey D (1995) Multiplexed single-photon timing array fluorometry: application to tryptophan fluorescence in microemulsions. PhD thesis, University of StrathclydeGoogle Scholar
  21. 21.
    Poland SP, Krstajić N, Monypenny J, Coelho S, Tyndall D, Walker RJ, Devauges V, Richardson J, Dutton N, Barber P, Day-Uei Li D, Suhling K, Ng T, Henderson RK, Ameer-Beg SM (2015) A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging. Biomed Opt Express 6:277.  https://doi.org/10.1364/BOE.6.000277CrossRefGoogle Scholar
  22. 22.
    Krstajić N, Levitt J, Poland S, Ameer-Beg S, Henderson R (2015) 256 × 2 SPAD line sensor for time resolved fluorescence spectroscopy. Opt Express 23:5653.  https://doi.org/10.1364/OE.23.005653CrossRefGoogle Scholar
  23. 23.
    Sagoo K, Hirsch R, Johnston P, McLoskey D, Hungerford G (2014) Pre-denaturing transitions in human serum albumin probed using time-resolved phosphorescence. Spectrochim Acta A 124:611Google Scholar
  24. 24.
    Polívka T, Sundstrom V (2005) Carotenoid excited states photophysics, ultrafast dynamics and photosynthetic functions. In: Di Bartolo B, Forte O (eds) Frontiers of optical spectroscopy: investigating extreme physical conditions with advanced optical techniques. Kluwer Academic, DordrechtGoogle Scholar
  25. 25.
    Bot F, Anese M, Lemos MA, Hungerford G (2015) Use of time-resolved spectroscopy as a method to monitor carotenoids present in tomato extract obtained using ultrasound treatment. Phytochem Anal 27:32Google Scholar
  26. 26.
    Fujii R, Onaka K, Nagae H, Koyama Y, Watanabe Y (2001) Fluorescence spectroscopy of all-trans-lycopene: comparison of the energy and the potential displacements of its 2 Ag state with those of neurosporene and spheroidene. J Lumin 92:213Google Scholar
  27. 27.
    Zhang J-P, Fujii R, Qian P, Inaba T, Mizoguchi T, Koyama Y, Onaka K, Watanabe Y (2000) Mechanism of the carotenoid-to-bacteriochlorophyll energy transfer via the S1 state in the LH2 complexes from purple bacteria. J Phys Chem B 104:3683Google Scholar
  28. 28.
    Linsinger TPJ, Roebben G, Gilliland D, Calzolai L, Rossi F, Gibson N, Klein C (2012) Requirements on measurements for the implementation of the European Commission definition of the term “nanomaterial”. European Commission Joint Research Centre Report.  https://doi.org/10.22787/63490
  29. 29.
    Steiner RF (1991) Fluorescence anisotropy: theory and applications. In: Lakowicz JR (ed) Principles. Topics in fluorescence spectroscopy, vol 2. Plenum, New York, p 1Google Scholar
  30. 30.
    Kawski A (1993) Fluorescence anisotropy: theory and applications of rotational depolarization. Crit Rev Anal Chem 23:459Google Scholar
  31. 31.
    Smith TA, Ghiggino KP (2015) A review of the analysis of complex time-resolved fluorescence anisotropy data. Methods Appl Fluoresc 3:022001Google Scholar
  32. 32.
    Weber G (1952) Polarization of the fluorescence of macromolecules: I. Theory and experimental method. Biochem J 52:145Google Scholar
  33. 33.
    Iler RK (1979) The chemistry of silica: solubility, polymerization, colloid and surface properties, and biochemistry. Wiley, New YorkGoogle Scholar
  34. 34.
    Brinker CJ, Scherer GW (1990) Sol-gel science. Academic Press, LondonGoogle Scholar
  35. 35.
    Apperson K, Karolin J, Martin RW, Birch DJS (2009) Nanoparticle metrology standards based on the time resolved fluorescence anisotropy of silica colloids. Meas Sci Technol 20:025310Google Scholar
  36. 36.
    Laursen BW, Krebs FC (2001) Synthesis, structure, and properties of azatriangulenium salts. Chem Eur J 7:1773Google Scholar
  37. 37.
    Sorensen TJ, Thyrhaug E, Szabelski M, Luchowski R, Gryczynski I, Gryczynski Z, Laursen BW (2013) Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay. Methods Appl Fluoresc 1:025001Google Scholar
  38. 38.
    Stewart HL, Yip P, Rosenberg M, Sørensen TJ, Laursen BW, Knight AE, Birch DJS (2016) Nanoparticle metrology of silica colloids and super-resolution studies using the ADOTA fluorophore. Meas Sci Technol 27:045007Google Scholar
  39. 39.
    Karolin J, Geddes CD, Wynne K, Birch DJS (2002) Nanoparticle metrology in sol-gels using multiphoton excited fluorescence. Meas Sci Technol 13:21Google Scholar
  40. 40.
    Lemos MA, Hungerford G (2013) The binding of curcuma longa extract with bovine serum albumin monitored via time-resolved fluorescence. Photochem Photobiol 89:1071Google Scholar
  41. 41.
    Köllner M, Wolfrum J (1992) How many photons are necessary for fluorescence lifetime measurements? Chem Phys Lett 200:199Google Scholar
  42. 42.
    Hungerford G, Allison A, McLoskey D, Kuimova MK, Yahioglu G, Suhling K (2009) Monitoring sol-to-gel transitions via fluorescence lifetime determination using viscosity sensitive fluorescent probes. J Phys Chem B 113:12067Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • David J. S. Birch
    • 1
    • 2
    Email author
  • Graham Hungerford
    • 2
  • David McLoskey
    • 2
  • Kulwinder Sagoo
    • 2
  • Philip Yip
    • 1
    • 2
  1. 1.Photophysics Research Group, Department of PhysicsScottish Universities Physics Alliance, University of StrathclydeGlasgowUK
  2. 2.HORIBA Jobin Yvon IBH LtdGlasgowUK

Personalised recommendations