Unveiling Biophysical and Biological Properties of a Hypothetical Membrane Receptor by Exploiting Recent Imaging Advances

Chapter
First Online:
Part of the Springer Series on Fluorescence book series (SS FLUOR, volume 13)

Abstract

Fluorescence microscopy is indispensable in the study of biological systems at various length scales. This rapidly evolving field continues to offer researchers cutting-edge techniques that enhance spatial and temporal resolution, especially with the invention of superresolution methodologies. In this chapter, we focus on techniques that have aided in the understanding of various cell biological phenomena. Each technique has certain boundaries of spatial and temporal resolution, and fluorophore density in a particular biological sample may limit the applicability of some techniques. We discuss strengths and weaknesses of many such techniques by considering their use in understanding the biological function of a hypothetical membrane receptor. We conclude that a combination of techniques is required to fully understand any cell biological process.

Keywords

Fluorescence Light microscopy Molecular Interactions Spectroscopy Superresolution microscopy 
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Notes

Acknowledgments

We would like to thank Steve Vogel for valuable discussions and Travis Crites for critical reading of the manuscript.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Laboratory of Systems BiologyNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

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