Probes for Nanoscopy: Photoswitchable Fluorophores

  • Pedro F. Aramendía
  • Mariano L. Bossi
Part of the Springer Series on Fluorescence book series (SS FLUOR, volume 14)


In recent years, new concepts have emerged for imaging in a far-field fluorescence microscope with resolution under the diffraction limit. All these concepts bear in common the use of molecular states of the probe to switch its signal between a fluorescent and a dark state. So far, in these techniques different kinds of molecular switches have been applied, whose photochemical features become a crucial fact for the success. In this chapter, we will discuss how the two isomeric forms of a photochromic system can be used to design a fluorescent switch for that purpose. We will focus on the photochemical and photophysical relevant properties for these systems to fulfill the requirements of a suitable probe for the different strategies currently used in fluorescence nanoscopy. Examples containing diverse photochromes and their application in super-resolution fluorescence imaging will be described.


Imaging Microscopy Photomodulation Photochromism Photoswitching 


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© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.INQUIMAE—Departamento de Química Inorgánica, Analítica y Química Física, FCENUniversidad de Buenos AiresBuenos AiresArgentina

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