Chapter

Lanthanide Luminescence

Volume 7 of the series Springer Series on Fluorescence pp 47-88

Date:

Stable Luminescent Chelates and Macrocyclic Compounds

  • G. MathisAffiliated withCISbio Bioassays
  • , H. BazinAffiliated withCISbio Bioassays Email author 

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Abstract

This review is focused on the lanthanide probes usable for time-resolved luminescence resonance energy transfer experiment and in the development of bioassays. The basic principle of heterogeneous time-resolved fluorescence (TRF) assays and homogeneous TRF assays are summarized. The criteria that should fulfill a lanthanide luminescent probe to be useful in the design of bioassays in diagnostic or drug discovery (high throughput screening) are defined as brightness, absorption wavelength, luminescence decay, instrumentation crosstalk, stability, lipophilicity/hydrophilicity, photobleaching, quenching phenomenon, conjugation chemistry, and synthesis practicability. The photophysical properties and the fulfillment of the above criteria are commented for the most representative structures insisting on the available stability data. Two main groups of molecules are described: (1) the luminescent stable chelates and (2) the macrocyclic-based ligands. The stable chelates are based on EDTA, DTPA, podant-like scaffold, and peptide scaffolds. The macrocyclic compounds described are macrocycles, macrocycles with pendant groups, and macropolycyclic cage ligands (cryptands). Applications of lanthanides complexes to cell based assays as well as time-resolved microscopy and imaging are discussed.

Keywords

Fluoroimmunoassays High throughput screening Homogeneous time-resolved fluorescence Lanthanide chelates Lanthanide cryptates