Studies of DNA-Protein Interactions at the Single Molecule Level with Magnetic Tweezers

  • J.-F. Allemand
  • D. Bensimon
  • G. Charvin
  • V. Croquette
  • G. Lia
  • T. Lionnet
  • K.C. Neuman
  • O.A. Saleh
  • H. Yokota
Part of the Lecture Notes in Physics book series (LNP, volume 711)


The development of tools to manipulate and study single biomolecules (DNA, RNA, proteins) has opened a new vista on the study of their mechanical properties and their joint interactions. In this short review we will focus on (single and double stranded) DNA and its interactions with various classes of proteins: structural DNA binding proteins such as gene repressors (e.g., the Galactose Repressor, GalR) and mechano-chemical enzymes that alter the DNA’s topology (topoisomerases), unwind it (helicases) or translocate it (FtsK). We will show how the new tools at our disposal can be used to gain an unprecedented description of the binding properties (on and off-times) and the enzymes’ kinetic constants that are often out of reach of more classical, bulk techniques.


Single Molecule Persistence Length Single Molecule Level Magnetic Tweezer Single Biomolecule 
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Copyright information

© Springer 2007

Authors and Affiliations

  • J.-F. Allemand
    • 1
  • D. Bensimon
    • 1
  • G. Charvin
    • 1
  • V. Croquette
    • 1
  • G. Lia
    • 2
  • T. Lionnet
    • 1
  • K.C. Neuman
    • 1
  • O.A. Saleh
    • 3
  • H. Yokota
    • 4
  1. 1.Laboratoire de Physique Statistique and Department of BiologieEcole Normale Superieure, UMR 8550 CNRSParis Cedex 05France
  2. 2.Harvard University Chemistry and Chemical BiologyCambridgeUSA
  3. 3.Materials Department and Biomolecular Science and Engineering ProgramUniversity of California, Materials DeptUSA
  4. 4.Department of Molecular PhysiologyThe Tokyo Metropolition Institute of Medical ScienceTokyoJapan

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