Towards Knowledge Discovery from cDNA Microarray Gene Expression Data

  • Jan Komorowski
  • Torgeir R. Hvidsten
  • Tor-Kristian Jenssen
  • Dyre Tjeldvoll
  • Eivind Hovig
  • Arne K. Sandvik
  • Astrid Lægreid
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 1910)

Abstract

The advent of the so-called cDNA microarrays has offered the first possibility to obtain a global understanding of biological processes in living organisms by simultaneous readouts of tens of thousands of genes. Initial experiments suggest that genes with similar function have similar expression patterns in microarray experiments. Until now, most approaches to computational analysis of gene expressions have used unsupervised learning. Although in some cases unsupervised methods may be suficient, the complexity of the biological processes is so high that it is unlikely that purely syntactical analyses are capable of fully exploiting the richness of the microarray data. In addition, it seems natural to re-use the existing biological (background) knowledge. In this paper, we present some elements of a methodology for knowledge discovery from microarray experiments. Two source of bio-medical knowledge are used: Ashburner’s gene ontology and our own literature-derived network of gene-gene relations obtained by analysing Medline citation records. Predictive models can be induced and their classification quality validated through the ROC/AUC analysis and applied to provide hypotheses regarding the function of unclassified genes. The methodology has been so far tested on publicly available gene expression data and its results evaluated by molecular biologists and medical researchers.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Jan Komorowski
    • 1
  • Torgeir R. Hvidsten
    • 1
  • Tor-Kristian Jenssen
    • 1
  • Dyre Tjeldvoll
    • 1
  • Eivind Hovig
    • 2
  • Arne K. Sandvik
    • 3
  • Astrid Lægreid
    • 3
  1. 1.Knowledge Systems Group Department of Information and Computer ScienceNorwegian University of Science and TechnologyTrondheimNorway
  2. 2.Department of Tumor BiologyInstitute for Cancer ResearchOsloNorway
  3. 3.Department of Physiology and Biomedical EngineeringNorwegian University of Science and TechnologyTrondheimNorway

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