Reconstructing the Optical Thickness from Hoffman Modulation Contrast Images
Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.
Unable to display preview. Download preview PDF.
- 1.C. Preza, E.B. van Munster, J.A. Aten, D.L. Snyder, and F.U. Rosenberger. Determination of direction-independent optical path-length distribution of cells using rotational-diversity transmitted-light differential interference contrast (dic) images. In C.J. Cogswell, J.A. Conchello, and T. Wilson, editors, Three-Dimensional Microscopy: Image Acquisition and Processing V, volume 3261 of Proceedings of SPIE, pages 60–70. SPIE, 1998.Google Scholar
- 3.A.N. Thikonov and V.Y. Arseninn. Solution of ill-posed problems. V.H. Winston & Sons, John Wiley, 1977.Google Scholar
- 6.R. Hoffman. The modulation contrast microscope: principles and performance. Journal of Microscopy, 110:205–222, August 1977.Google Scholar
- 8.S. Bradbury. An Introduction to the Optical Microscope, volume 01 of Royal Microscopical Society MICROSCOPY HANDBOOKS. BIOS Scientific Publishers Ltd, 9 Newtec Place, Magdalen Road, Oxford OX4 1RE, UK, revised edition, 1989.Google Scholar
- 9.W.T. Grandy Jr. Scattering of Waves from Large Spheres. Cambridge University Press, 2000.Google Scholar
- 10.M. Born and E. Wolf. Principles of Optics, Electromagnetic Theory of Propagation, Interference and Diffraction of Light. Cambridge University Press, seventh (expanded) edition, 1999.Google Scholar
- 12.X. Zabulis, J. Sporring, and S.C. Orphanoudakis. Scale summarized and focused browsing of primitive visual content. In Proceedings of the Fourth International Conference on Visual Information Systems, volume 1929 of Lecture Notes in Computer Science, pages 269–278, Lyon, Prance, 2000. Springer Verlag.Google Scholar