Rotavirus Proteins: Structure and Assembly
Rotavirus is a major pathogen of infantile gastroenteritis. It is a large and complex virus with a multilayered capsid organization that integrates the deter minants of host specificity, cell entry, and the enzymatic functions necessary for endogenous transcription of the genome that consists of 11 dsRNA segments. These segments encode six structural and six nonstructural proteins. In the last few years, there has been substantial progress in our understanding of both the structural and functional aspects of a variety of molecular processes involved in the replication of this virus. Studies leading to this progress using of a variety of structural and biochemical techniques including the recent application of RNA interference technology have uncovered several unique and intriguing features related to viral morphogenesis. This review focuses on our current understanding of the structural basis of the molecular processes that govern the replication of rotavirus.
KeywordsNonstructural Protein Rice Dwarf Virus dsRNA Segment Endogenous Transcription Cell Entry Process
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- Estes MK (2001) Rotaviruses and their replication. In: Fields BN, Knipe RM, Chanock MS et al (eds) Virology. Lippincott-Raven, Philadelphia, pp 1747–1785Google Scholar
- Estes MK (ed) (2003) The rotavirus NSP4 enterotoxin: Current status and challenges. Elsevier, AmsterdamGoogle Scholar
- Estes MK, Crawford SE, Penaranda ME, Petrie BL, Burns JW, Chan WK, Ericson B, Smith GE, Summers MD (1987) Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system. JVirol 61:1488–1494Google Scholar
- Fields BN (1996) The Reoviridae. In: Fields BN, Knipe RM, Chanock MS et al (eds) Virology. Lippincott-Raven, Philadelphia, pp 1553–1555Google Scholar
- Iturriza-Gomara M, Auchterlonie IA, Zaw W, Molyneaux P, Desselberger U, Gray J (2002) Rotavirus gastroenteritis and central nervous system (CNS) infection: characterization of the VP7 and VP4 genes of rotavirus strains isolated from paired fecal and cerebrospinal fluid samples from a child with CNS disease. J Clin Microbiol 40:4797–4799PubMedGoogle Scholar
- Mackow ER, Shaw RD, Matsui SM, Vo PT, Dang MN, Greenberg HB (1988) The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proc Natl Acad Sci U S A 85:645–649PubMedGoogle Scholar
- Moon HW(1994) Pathophysiology of viral diarrhea. In: Kapikian AZ (ed) Viral infections of the gastrointestinal trac. Marcel Dekker, New York, pp 27–52Google Scholar
- Pesavento JB, Estes MK, Prasad BV (2003b) Structural organization of the genome in rotavirus. In: Desselberger U (ed) Perspectives in medical virology 9: viral gastroenteritis Elsevier, London, pp 115–127Google Scholar
- Prasad BVV, Estes MK (2000) Electron cryomicroscopy and computer image processing techniques: use in structure-function studies of rotavirus. Human Press, Totowa, NJGoogle Scholar
- Silvestri LS, Taraporewala ZF, Patton JT (2004) Rotavirus replication: plus-sense templates for double-stranded RNA synthesis are made in viroplasms. JVirol 78:7763–7774Google Scholar