Reoviruses: Entry, Assembly and Morphogenesis pp 117-167

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 309)

Reovirus Structure and Morphogenesis

Abstract

Assembly of a mature infectious virion from component parts is one of the last steps in the replicative cycle of most viruses. Recent advances in delineating aspects of this process for the mammalian orthoreoviruses (MRV), nonenveloped viruses composed of a genome of ten segments of double-stranded RNA enclosed in two concentric icosahedral protein capsids, are discussed. Analyses of temperature-sensitive (ts) assembly-defective reovirus mutants have been used to better understand requirements for viral inclusion formation and capsid morphogenesis. Newly determined high-resolution structures of virtually all MRV proteins, combined with complete MRV genomic sequence information and elucidation of sequence lesions in tsmutants, is now providing a context formolecularly understanding interactions that promote, or abrogate, reovirus capsid assembly. Additional advances in understanding required signals for whole genome construction fromsets of the ten individual genes, and in transcapsidation of subviral particles with engineered outer capsid proteins, provide additional molecular genetic understanding of reovirus protein structure-function and morphogenesis.

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© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  1. 1.Department of Medical Microbiology and Infectious DiseasesUniversity of ManitobaWinnipegCanada

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