In Vitro Culture of Mycorrhizas pp 73-91

Part of the Soil Biology book series (SOILBIOL, volume 4)

Life History Strategies in Gigasporaceae: Insight from Monoxenic Culture

  • Francisco Adriano de Souza
  • Yolande Dalpé
  • Stéphane Declerck
  • Ivan Enrique de la Providencia
  • Nathalie Séjalon-Delmas


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  1. Bago B, Zipfel W, Williams RM, Piché Y (1999) Nuclei of symbiotic arbuscular mycorrhizal fungi as revealed by in vivo two-photon microscopy. Protoplasma 209:77–89PubMedGoogle Scholar
  2. Bécard G, Piché Y (1989a) New aspects on the acquisition of biotrophic status by a vesicular-arbuscular mycorrhizal fungus, Gigaspora margarita. New Phytol 112:77–83Google Scholar
  3. Bécard G, Piché Y (1989b) Fungal growth stimulation by CO2 and root exudates in vesicular-arbuscular mycorrhizal symbiosis. Appl Environ Microbiol 55:2320–2325PubMedGoogle Scholar
  4. Beena KR, Raviraja NS, Arun AB, Sridhar KR (2000) Diversity of arbuscular mycorrhizal fungi on the coastal sand dunes of the west coast of India. Curr Sci 79:1459–1466Google Scholar
  5. Begon M, Harper JL, Townsend CR (1996) Ecology: individual, populations and communities, 3rd edn. Blackwell, LondonGoogle Scholar
  6. Beilby JP, Kidby DK (1980) Biochemistry of ungerminated and germinated spores of the vesicular-arbuscular mycorrhizal fungus, Glomus caledonium: changes in neutral and polar lipids. J Lipid Res 21:739–750PubMedGoogle Scholar
  7. Biermann BJ, Linderman RG (1983) Use of vesicular-arbuscular mycorrhizal roots, intraradical vesicles and extraradical vesicles as inoculum. New Phytol 95:97–105Google Scholar
  8. Boovaraghan B, Poulin MJ, Vierheilig H, Piché Y (1995) Responses of an arbuscular mycorrhizal fungus, Gigaspora margarita, to exudates and volatiles from the Ri T-DNA-transformed roots of nonmycorrhizal and mycorrhizal mutants of Pisum sativum L. Sparkle. Exp Mycol 19:275–283Google Scholar
  9. Braunberger PG, Abbott LK, Robson AD (1996) Infectivity of arbuscular mycorrhizal fungi after wetting and drying. New Phytol 134:673–684Google Scholar
  10. Brundrett MC, Jasper DA, Ashwath N (1999) Glomalean mycorrhizal fungi from tropical Australia. II. The effect of nutrient levels and host species on the isolation of fungi. Mycorrhiza 8:315–321Google Scholar
  11. Buée M, Rossignol M, Jauneau A, Ranjeva R, Bécard G (2000) The pre-symbiotic growth of arbuscular mycorrhizal fungi is induced by a branching factor partially purified from plant root exudates. Mol Plant-Microb Interact 13:693–698Google Scholar
  12. David-Schwartz R, Badani H, Wininger S, Levy AA, Galili G, Kapulnik Y (2001) Identification of a novel genetically controlled step in mycorrhizal colonization: plant resistance to infection by fungal spores but not to extraradical hyphae. Plant J 27:561–569PubMedCrossRefGoogle Scholar
  13. de la Providencia I, de Souza FA, Fernandez F, Sejalon Delmas N, Declerck S (2005) Arbuscular mycorrhizal fungi reveal distinct pattern of anastomoses formation and hyphal healing mechanism between different phylogenic groups. New Phytologist 165:261–271PubMedGoogle Scholar
  14. de Souza FA, Declerck S (2003) Mycelium development and architecture, and spore production of Scutellospora reticulata in monoxenic culture with Ri T-DNA transformed carrot roots. Mycologia 95:1004–1012Google Scholar
  15. de Souza FA, Kowalchuk GA, Leeflang P, van Veen JA, Smit E (2004) PCR-denaturing gradient gel electrophoresis profiling of inter-and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora. Appl Environ Microbiol 70:1413–1424PubMedGoogle Scholar
  16. Declerck S, Strullu DG, Plenchette C (1998) Monoxenic culture of the intraradical forms of Glomus sp. isolated froma tropical ecosystem: a proposed methodology for germplasm collection. Mycologia 90:579–585Google Scholar
  17. Declerck S, Cranenbrouck S, Dalpé Y, Séguin S, Grandmougin-Ferjani A, Fontaine J, Sancholle M (2000) Glomus proliferum sp. nov.: a description based on morphological, biochemical, molecular and monoxenic cultivation data. Mycologia 92:1178–1187Google Scholar
  18. Declerck S, D’Or D, Cranenbrouck S, Le Boulengé E (2001) Modeling the sporulation dynamics of arbuscular mycorrhizal fungi in monoxenic culture. Mycorrhiza 11:225–230Google Scholar
  19. Declerck S, D’Or D, Bivort C, de Souza FA (2004) Development of extraradical mycelium of Scutellospora reticulata under root-organ culture: spore production and function of auxiliary cells. Mycol Res 108:84–92PubMedCrossRefGoogle Scholar
  20. Diop TA, Bécard G, Piché Y (1992) Long-term in vitro culture of an endomycorrhizal fungus, Gigaspora margarita, on Ri T-DNA transformed roots of carrot. Symbiosis 12:249–259Google Scholar
  21. Douds DD, Nagahashi G, Abney GD (1996). The differential effects of cell-wall associated phenolics, cell walls and cytosolic phenolics of host and non-host roots on the growth of two species of AM fungi. New Phytol 133:289–294Google Scholar
  22. Fairchild MH, Miller GL (1990) Vesicular-arbuscularmycorrhizas and the soil-disturbance-induced reduction of nutrient absorption in maize. III. Influence of P amendments to soil. New Phytol 114:641–650Google Scholar
  23. Fortin JA, Bécard G, Declerck S, Dalpé Y, St-Arnaud M, Coughlan AP, Piché Y (2002) Arbuscular mycorrhiza on root-organ cultures. Can J Bot 80:1–20CrossRefGoogle Scholar
  24. Gadkar V, Adholeya A (2000) Intraradical sporulation of AM Gigaspora margarita in long-term axenic cultivation in Ri T-DNA carrot root. Mycol Res 104:716–721CrossRefGoogle Scholar
  25. Gadkar V, David-Schwartz R, Nagahashi G, Douds DD, Wininger S, Kapulnik Y (2003) Root exudate of pmi tomato mutant M161 reduces AM fungal proliferation in vitro. FEMS Microbiol Lett 223:193–198PubMedCrossRefGoogle Scholar
  26. Gemma JN, Koske RE (1988) Pre-infection interactions between roots and the mycorrhizal fungi Gigaspora gigantea: chemotropism of germ tubes and root growth responses. Trans Br Mycol Soc 91:122–132Google Scholar
  27. Gerdermann JW (1955) Wound-healing of hyphae in a phycomycetousmycorrhizal fungus. Mycologia 47:916–918Google Scholar
  28. Giovannetti M, Azzolini D, Citernesi AS (1999) Anastomosis formation and nuclear and protoplasmic exchange in arbuscular mycorrhizal fungi. Appl Environ Microbiol 65:5571–5575PubMedGoogle Scholar
  29. Giovannetti M, Sbrana C, Logi C (2000) Microchambers and video-enhanced light microscopy for monitoring cellular events in living hyphae of arbuscular-mycorrhizal fungi. Plant Soil 226:153–159CrossRefGoogle Scholar
  30. Giovannetti M, Sbrana C, Strani P, Agnolucci M, Rinaudo V, Avio L (2003) Genetic diversity of isolates of Glomus mosseae from different geographic areas detected by vegetative compatibility testing and biochemical and molecular analysis. Appl Environ Microbiol 69:616–624PubMedCrossRefGoogle Scholar
  31. Grime JP (1979) Plant strategies and vegetation process. Wiley, New YorkGoogle Scholar
  32. Harrison MJ (1999) Molecular and cellular aspects of the arbuscular mycorrhizal symbiosis. Annu Rev Plant Physiol 50:361–389Google Scholar
  33. Hart MM, Klironomos JN (2002) Diversity of arbuscular mycorrhizal fungi and ecosystem functioning In: van der Heijden MGA, Sanders IR (eds) Mycorrhizal ecology, vol 157. Springer, Berlin Heidelberg New York, pp 225–242Google Scholar
  34. Hart MM, Reader RJ (2002) Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytol 153:335–344CrossRefGoogle Scholar
  35. Hart MM, Reader RJ, Klironomos JN (2001) Life-history strategies of arbuscular mycorrhizal fungi in relation to their successional dynamics. Mycologia 93:1186–1194Google Scholar
  36. Helgason T, Daniell TJ, Husband R, Fitter AH, Young JPW (1998) Ploughing up the wood-wide web? Nature 394:431PubMedCrossRefGoogle Scholar
  37. Jansa J, Mozafar A, Anken T, Ruh R, Sanders IR, Frossard E (2002) Diversity and structure of AMF communities as affected by tillage in a temperate soil. Mycorrhiza 12:225–234PubMedGoogle Scholar
  38. Jasper DA, Abbott LK, Robson AD (1989) Hyphae of a vesicular-arbuscular mycorrhizal fungus maintain infectivity in dry soil, except when the soil is disturbed. New Phytol 112:101–107Google Scholar
  39. Kang-Hyeon K, Chang-Duck K, Chang-Keun Y (1994) Hyphal growth, auxiliary cell development and hyphal healing process of arbuscular mycorrhizal fungi, Gigaspora and Scutellospora genera. Korean J Mycol 22:36–45Google Scholar
  40. Klironomos JN, Hart MM (2002) Colonization of roots by arbuscular mycorrhizal fungi using different sources of inoculum. Mycorrhiza 12:181–184PubMedGoogle Scholar
  41. Koch AM, Kuhn G, Fontanillas P, Fumagalli L, Goudet I, Sanders IR (2004) High genetic variability and low local diversity in a population of arbuscular mycorrhizal fungi. Proc Natl Acad Sci USA 101:2369–2374PubMedGoogle Scholar
  42. Koske RE (1981a) Multiple germinations by spores of Gigaspora gigantea. Trans Br Mycol Soc 76:328–330Google Scholar
  43. Koske RE (1981b) Gigaspora gigantea: observations on spore germination of a VA-mycorrhiza fungus. Mycologia 73:288–300Google Scholar
  44. Koske RE (1982) Evidence for a volatile attractant from plant roots affecting germ tubes of a VA mycorrhizal fungus. Trans Br Mycol Soc 79:305–310CrossRefGoogle Scholar
  45. Lee PJ, Koske RE (1994) Gigaspora gigantea: seasonal abundance and ageing of spores in a sand dune. Mycol Res 98:453–457Google Scholar
  46. Lovelock CE, Andersen K, Morton JB (2003) Arbuscular mycorrhizal communities in tropical forests are affected by host tree species and environment. Oecologia 135:268–279PubMedGoogle Scholar
  47. Maia LC, Yano-Melo AM (2001) Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates. Braz JMicrobiol 32:281–285Google Scholar
  48. McArthur RH, Wilson EO (1967) The theory of island biogeography. Princeton University Press, PrincetonGoogle Scholar
  49. Morton JB (1995) Taxonomic and phylogenetic divergence among five Scutellospora species based on comparative developmental sequences. Mycologia 87:127–137Google Scholar
  50. Nagahashi G, Douds DD (1999) A rapid and sensitive bioassay with practical application for studies on interactions between root exudates and arbuscular mycorrhizal fungi. Biotechnol Tech 13:893–897CrossRefGoogle Scholar
  51. Nagahashi G, Douds DD (2000) Partial separation of the root exudates components and their effects upon the growth of germinated spores of AM fungi. Mycol Res 104:1453–1464CrossRefGoogle Scholar
  52. Nagahashi G, Douds DD, Abney GD (1996) Phosphorus amendment inhibits hyphal branching of the VAM fungus Gigaspora margarita directly and indirectly through its effect on root exudation. Mycorrhiza 6:403–408CrossRefGoogle Scholar
  53. Nair MG, Safir GR, Siqueira JO (1991) Isolation and identification of vesicular-arbusculalr mycorrhiza-stimulatory compounds fromClover (Trifoliumrepens) roots. Appl Environ Microbiol 57:434–439PubMedGoogle Scholar
  54. Pawlowska TE, Douds DD, Charvat I (1999) In vitro propagation and life cycle of the arbuscular mycorrhizal fungus Glomus etunicatum. Mycol Res 103:1549–1556CrossRefGoogle Scholar
  55. Pianka ER (1970) On r and k selection. Am Nat 104:592–597CrossRefGoogle Scholar
  56. Pringle A, Bever JD (2002) Divergent phenologies may facilitate the coexistence of arbuscular mycorrhizal fungi in a North Carolina grassland. Am J Bot 89:1439–1446Google Scholar
  57. Pringle A, Taylor JW (2002) The fitness of filamentous fungi. Trends Microbiol 10:474–481PubMedCrossRefGoogle Scholar
  58. Romero AGF, Siqueira JO (1996) Activity of flavonoids on spores of the mycorrhizal fungus Gigaspora gigantea in vitro. Pesqui Agropecu Bras 31:517–522Google Scholar
  59. Santos AL, de Souza FA, Guerra JGM, Berbara RLL (2000) Estabelecimento e capacidade infectiva de Gigaspora margarita e Glomus clarum em solo sob erosão. Acta Bot Bras 14:127–139Google Scholar
  60. Schenck NC, Perez Y (1990) Manual for the identification of VA mycorrhizal fungi. Synergistic Publications, GainesvilleGoogle Scholar
  61. Schwarzott D, Walker C, Schußler A (2001) Glomus, the largest genus of the arbuscular mycorrhizal fungi (Glomales), is nonmonophyletic. Mol Phylogenet Evol 21:190–197PubMedGoogle Scholar
  62. Sieverding E (1991) Vesicular-arbuscular mycorrhiza management in tropical agrosystems. Germany. Deutsche Gesellschaft für Technische Zusammenarbeit, Eschborn, GermanyGoogle Scholar
  63. Siqueira JO, Colozzi FA, de Oliveira (1989) Occurrence of vesicular-arbuscular mycorrhizae in agro and natural ecosystems in Minas Gerais state. Pesqui Agropecu Bras 24:1499–1506Google Scholar
  64. Smith SE, Read DJ (1997) Mycorrhizal symbiosis. Academic Press, LondonGoogle Scholar
  65. Spain JL, Sieverding E, Schenck NC (1989) Gigaspora ramisporophora: a new species with novel sporophores from Brazil. Mycotaxon 34:667–677Google Scholar
  66. Stürmer SL, Bellei MM (1994) Composition and seasonal variation of spore populations of arbuscular mycorrhizal fungi in dune soils on the island of Santa Catarina, Brazil. Can J Bot 72:359–363Google Scholar
  67. Stutz JC, Copeman R, Martin CA, Morton JB (2000) Patterns of species composition and distribution of arbuscular mycorrhizal fungi in arid regions of southwestern North America and Namibia, Africa. Can J Bot 78:237–245CrossRefGoogle Scholar
  68. Suriyapperuma SP, Koske RE (1995) Attraction of germ tubes and germination of spores of the arbuscular mycorrhizal fungus Gigaspora gigantea in the presence of roots of maize exposed to different concentrations of phosphorus. Mycologia 87:772–778Google Scholar
  69. Tamasloukht B, Séjalon-Delmas N, Kluever A, Roux C, Bécard G, Franken P (2003) Root factor induce mitochondrial-related-gene expression and fungal respiration during the developmental switch from asymbiosis to presymbiosis in the arbuscular mycorrhizal fungus Gigaspora rosea. Plant Physiol 131:1468–1478PubMedCrossRefGoogle Scholar
  70. Tiwari P, Adholleya A (2002) Invitro co-culture of two AMF isolates Gigasporamargarita and Glomus intraradices on Ri-T-DNA transformed roots. FEMS Microbiol Lett 206:39–43PubMedCrossRefGoogle Scholar
  71. Tommerup IC (1988) The vesicular-arbuscular mycorrhizas. Adv Plant Pathol 6:81–91Google Scholar
  72. Tommerup IC, Abbott LK (1981) Prolonged survival and viability of vesicular-arbuscular mycorrhizae hyphae after root death. Soil Biol Biochem 13:431–434CrossRefGoogle Scholar
  73. van Tuinen D, Jacquot E, Zhao B, Gollotte A, Gianinazzi-Pearson V (1998) Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25S rDNA-targeted nested PCR. Mol Ecol 7:879–887PubMedGoogle Scholar
  74. Walker C, Sanders FE (1986) Taxonomic concepts in the Endogonaceae. III. The separation of Scutellospora gen. nov. from Gigaspora Gerd. & Trappe. Mycotaxon 27:169–182Google Scholar
  75. Zhao ZW, Wang GH, Yang L (2003) Biodiversity of arbuscular mycorrhizal fungi in a tropical rainforest of Xishuangbanna, southwest China. Fungal Divers 13:233–242Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Francisco Adriano de Souza
    • 1
  • Yolande Dalpé
    • 2
  • Stéphane Declerck
    • 3
  • Ivan Enrique de la Providencia
    • 3
    • 4
  • Nathalie Séjalon-Delmas
    • 5
  1. 1.Empresa Brasileira de Pesquisa Agropecuária — Embrapa AgrobiologiaSeropédica, RJBrazil
  2. 2.Agriculture and Agri-Food Canada Environment Health/BiodiveristyOttawaCanada
  3. 3.Mycothèque de l’Université Catholique de Louvain (MUCL), Unité de MicrobiologieUniversité Catholique de LouvainLouvain-La-NeuveBelgium
  4. 4.Km 3 carretera de TapasteInstituto Nacional de Ciencias Agricolas (INCA)La HabanaCuba
  5. 5.Equipe deMycologie VégétaleUMR 5546 CNRS Université Paul Sabatier, Pôle de Biotechnologie VégétaleCastanet-TolosanFrance

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