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High-Throughput RNA Interference in Functional Genomics

  • M. Janitz
  • D. Vanhecke
  • H. Lehrach
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 173)

Abstract

RNA interference (RNAi) refers to post-transcriptional silencing of gene expression as a result of the introduction of double-stranded RNA into cells. The application of RNAi in experimental systems has significantly accelerated elucidation of gene functions. In order to facilitate large-scale functional genomics studies using RNAi, several high-throughput approaches have been developed based on microarray or microwell assays. The recent establishment of large libraries of RNAi reagents combined with a variety of detection assays has further improved the performance of functional genome-wide screens in mammalian cells.

Keywords

RNA interference Microarray Reverse transfection Cell array 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • M. Janitz
    • 1
  • D. Vanhecke
    • 1
  • H. Lehrach
    • 1
  1. 1.Department Vertebrate GenomicsMax Planck Institute for Molecular GeneticsBerlinGermany

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