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Locked Nucleic Acid: High-Affinity Targeting of Complementary RNA for RNomics

  • S. Kauppinen
  • B. Vester
  • J. Wengel
Chapter
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 173)

Abstract

Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards complementary single-stranded RNA molecules. That makes fully modified LNAs, LNA/DNA mixmers, or LNA/RNA mixmers uniquely suited for mimicking RNA structures and for RNA targeting in vitro or in vivo. The focus of this chapter is on LNA antisense, LNA-modified DNAzymes (LNAzymes), LNA-modified small interfering (si)RNA (siLNA), LNA-enhanced expression profiling by real-time RT-PCR and detection and analysis of microRNAs by LNA-modified probes.

Keywords

LNA Locked nucleic acid RNA targeting LNA antisense MicroRNA targeting 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • S. Kauppinen
    • 1
  • B. Vester
    • 2
  • J. Wengel
    • 3
  1. 1.Department of Functional Genomics, ExiqonVedbaekDenmark
  2. 2.Nucleic Acid Center, Department of Biochemistry and Molecular BiologyUniversity of Southern DenmarkOdense MDenmark
  3. 3.Nucleic Acid Center, Department of ChemistryUniversity of Southern DenmarkOdense MDenmark

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