Abstract
Freshwater eutrophisation causes blooms of cyanobacteria, including species that produce toxins harmful to animals. We present here a study of the effects of two hepatotoxins: microcystin-LR (heptapeptide) and cylindrospermopsin (alkaloid), on Euglena, a photosynthetic protist. Microcystin- LR (0.01–10 εg ml−1) and cylindrospermopsin (0.13–12.5 εg ml−1) showed no toxic effect on growth but significantly increased cell productivity. O2 consumption was significantly stimulated half an hour after the toxin was added for microcystin and 48 h after for cylindrospermopsin for all the concentrations tested. In addition, a drastic inhibition of greening and photosynthesis as well as an 80% increase of reduced glutathione were observed at the high concentrations of cylindrosper-mopsin. Two-dimensional electrophoresis after 35S amino acid labeling showed that with cylindrospermopsin a 23-kDa protein was induced in the first 2 h, whereas a 29-kDa protein was overexpressed with microcystin and cylindrospermopsin.
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Duval, E., Coffinet, S., Bernard, C., Briand, J. (2005). Effects of Two Cyanotoxins, Microcystin-LR and Cylindrospermopsin, on Euglena gracilis. In: Lichtfouse, E., Schwarzbauer, J., Robert, D. (eds) Environmental Chemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26531-7_60
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DOI: https://doi.org/10.1007/3-540-26531-7_60
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