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Lessons from Cre-Mice and Indicator Mice

  • Christian Wolfrum
  • Leon Gabriel StraubEmail author
Chapter
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 251)

Abstract

The adult human adipose tissue is predominantly composed of white adipocytes. However, within certain depots, adipose tissue contains thermogenically active brown-like adipocytes, which have been evolutionarily conserved in mammals. This chapter will give a brief overview on the methods used to genetically target and trace both white and brown adipocytes using techniques such as bacterial artificial chromosome (BAC) cloning to create transgenic mouse models and the tools with which genetic recombination is mediated in vivo (e.g., Cre-loxP, CreERT, and Tet-On). The chapter furthermore critically discusses the strength and limitation of the various systems used to target mature white and brown adipocytes (ap2-Cre, Adipoq-Cre, and Ucp1-Cre). Based on these systems, it is evident that our knowledge of mature adipocyte categorization into brown, white, brite, or beige adipocytes is strongly influenced by the use of the various genetic mouse models described in this chapter. Our evaluation of different studies using the aforementioned systems focuses on key genes, which have been reported to maintain adipocyte’s function (insulin receptor, Raptor, or Atgl).

Keywords

AdipoChaser Adipocytes Adiponectin aP2 Atgl BAT Beige Brite Brown Cre Doxycycline FABP4 Indicator mouse Interconversion IR mTORC1 Raptor Rptor Tamoxifen Tet-On ThermoMouse Transdifferentiation Ucp1 Ucp1-Cre Ucp1-CreERT White 

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Authors and Affiliations

  1. 1.Institute of Food, Nutrition, and Health, ETH ZurichZürichSwitzerland

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