Activity-Based Protein Profiling pp 137-162

Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume 324)

Functional Analysis of Protein Targets by Metabolomic Approaches

Chapter

Abstract

Proteomics methods, such as activity-based protein profiling, can be used to connect proteins to biology and disease. Some proteins found through unbiased methods are not well characterized, which makes it difficult to ascertain the role of these proteins. Metabolomics approaches are useful in characterizing proteins that regulate or bind metabolites. Here, we provide examples of the development and use of metabolomics approaches to elucidate protein–metabolite interactions.

Keywords

Enzymes Liquid chromatography-mass spectrometry (LC-MS) Metabolite binding proteins Metabolomics 

Abbreviations

15d-PGJ2

15-Deoxy-Δ-12,14-prostaglandin-J2

ABPP

Activity-based protein profiling

ANS

1-Anilinonaphthalene-8-sulfonate

CRABP2

Cytosolic retinoic acid binding protein 2

DBD

DNA-binding domain

DMP

Discovery metabolite profiling

DPP4

Dipeptidyl peptidase 4

FAAH

Fatty acid amide hydrolase

FTMS

Fourier transform mass spectrometry

GST

Glutathione S-transferase

IDH

Isocitrate dehydrogenase

IDMS

Isotope dilution mass spectrometry

LBD

Ligand-binding domain

LC-MS

Liquid chromatography–mass spectrometry

LPA

Oleyl lysophosphatidic acid

MAGE

Monoalkylglycerol ether

MAGL

Monoacylglycerol lipase

NAE

N-acyl ethanolamine

NAT

N-acyl taurine

NMR

Nuclear magnetic resonance

PHI

Peptide histidine isoleucine

PMI

Protein–metabolite interactions

PPAR

Peroxisome proliferator-activated receptor

R132H

Arginine 132 switched to histidine

StarD3

StAR-related lipid transfer domain 3

THC

Tetrahydrocannabinol

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© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Department of Chemistry and Chemical BiologyHarvard UniversityCambridgeUSA

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