Microfluidics pp 153-169

Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume 304) | Cite as

A Nanomembrane-Based Nucleic Acid Sensing Platform for Portable Diagnostics

  • Satyajyoti Senapati
  • Sagnik Basuray
  • Zdenek Slouka
  • Li-Jing Cheng
  • Hsueh-Chia Chang
Chapter

Abstract

In this perspective article, we introduce a potentially transformative DNA/RNA detection technology that promises to replace DNA microarray and real-time PCR for field applications. It represents a new microfluidic technology that fully exploits the small spatial dimensions of a biochip and some new phenomena unique to the micro- and nanoscales. More specifically, it satisfies all the requisites for portable on-field applications: fast, small, sensitive, selective, robust, label- and reagent-free, economical to produce, and possibly PCR-free. We discuss the mechanisms behind the technology and introduce some preliminary designs, test results, and prototypes.

Keywords

Depletion Dielectrophoresis Ion-selective membranes Limiting-current 

Abbreviations

AC

Alternating current

CNT

Carbon nanotube

DC

Direct current

DEP

Dielectrophoresis

DNA

Deoxyribonucleic acid

FCW

Fluorescence correlation spectroscopy

FET

Field-effect transistor

IV

Current–Voltage

kb

Kilobase

PCR

Polymerase chain reaction

pM

Picomolar

RNA

Ribonucleic acid

SNP

Single-nucleotide polymorphism

ssDNA

Single-stranded DNA

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Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Satyajyoti Senapati
    • 1
  • Sagnik Basuray
    • 1
  • Zdenek Slouka
    • 1
  • Li-Jing Cheng
    • 1
  • Hsueh-Chia Chang
    • 1
  1. 1.Department of Chemical and Biomolecular EngineeringUniversity of Notre DameNotre DameUSA

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