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Knowledge Discovery in the Identification of Differentially Expressed Genes in Tumoricidal Macrophage

  • A. Fazel Famili
  • Ziying Liu
  • Pedro Carmona-Saez
  • Alaka Mullick
Part of the Lecture Notes in Computer Science book series (LNCS, volume 3646)

Abstract

High-throughput microarray data are extensively produced to study the effects of different treatments on cells and their behaviours. Understanding this data and identifying patterns of groups of genes that behave differently or similarly under a set of experimental conditions is a major challenge. This has motivated researchers to consider multiple methods to identify patterns in the data and study the behaviour of hundreds of genes. This paper introduces three methods, one of which is a new technique and two are from the literature. The three methods are cluster mapping, Rank Products and SAM. Using real data from a number of microarray experiments comparing the effects of two very different products that can activate macrophage tumoricidal activity we have identified groups of genes that share interesting expression patterns. These methods have helped us to gain an insight into the biological problem under study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • A. Fazel Famili
    • 1
  • Ziying Liu
    • 1
  • Pedro Carmona-Saez
    • 2
  • Alaka Mullick
    • 3
  1. 1.Institute for Information Technology National Research Council of CanadaOttawaCanada
  2. 2.Centro Nacional de Biotecnología (CNB – CSIC)MadridSpain
  3. 3.Biotechnology Research InstituteNational Research Council of CanadaMontrealCanada

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