An Inexpensive LED-Based Fluorometer Used to Study a Hairpin-Based DNA Nanomachine

  • Hanwen Yan
Part of the Lecture Notes in Computer Science book series (LNCS, volume 3384)

Abstract

Fluorometers have become indispensable tools in the study of DNA-based nanomachines. The cost of such an instrument is usually outside the budget of a high school science department or that of an amateur scientist. This paper presents a low-cost fluorometer that can be assembled for the cost of approximately a hundred dollars. By monitoring Fluorescence Resonance Energy Transfer (FRET) from the donor dye TET to the acceptor dye TAMRA, this fluorometer has been successfully used to follow the repeated opening and closing of a DNA hairpin nanomachine. This instrument makes possible the investigation of DNA-based nanotechnology or the performance of FRET-based molecular biology experiments within a high school setting.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Mao, C., Sun, W., Shen, Z., Seeman, N.C.: A nanomechanical device based on the B-Z transition of DNA. Nature 297, 144–146 (1999)Google Scholar
  2. 2.
    Yurke, B., Turberfield, A.J., Mills Jr., A.P., Simmel, F.C., Neumann, J.L.: A DNA-fuelled molecular machine made of DNA. Nature 406, 605–608 (2000)CrossRefGoogle Scholar
  3. 3.
    Simmel, F.C., Yurke, B.: Using DNA to construct and power a nanoactuator. Phys. Rev. E 63 art. no. 041913 (2001)Google Scholar
  4. 4.
    Simmel, F.C., Yurke, B.: A DNA-based molecular device switchable between three distinct mechanical states. Appl. Phys. Lett. 80, 883–885 (2002)CrossRefGoogle Scholar
  5. 5.
    Yan, H., Zhang, X., Shen, Z., Seeman, N.C.: A robust DNA mechanical device controlled by hybridization topology. Nature 415, 62–65 (2002)CrossRefGoogle Scholar
  6. 6.
    Li, J.J., Tan, W.: A single DNA molecular nanomotor. Nano Lett. 2, 315–318 (2002)CrossRefGoogle Scholar
  7. 7.
    Turberfield, A.J., Yurke, B., Mills Jr., A.P., Blake, M.I., Mitchel, J.C., Simmel, F.C.: Hybridization catalysis: controlled power for nanomachines. Phys. Rev. Lett. 90, art. no. 118102 (2002)Google Scholar
  8. 8.
    Alberti, P., Mergny, J.-L.: DNA duplex-quadruples exchange as the basis for a nanomolecular machine. PNAS 100, 1569–1573 (2003)CrossRefGoogle Scholar
  9. 9.
    Feng, L., Park, S.H., Reif, J.H., Yan, H.: A two-state DNA lattice switched by DNA nanoactuator. Angew. Chem. Int. Ed. 42, 4342–4346 (2003)CrossRefGoogle Scholar
  10. 10.
    Niemeyer, C., Adler, M.: Nanomechanical Devices Based on DNA. Angew. Chem. Int. Ed. 41, 3779–3783 (2002)Google Scholar
  11. 11.
    Jares-Erijman, E.A., Jovin, T.M.: FRET imaging. Nature Biotech. 21, 1387–1395 (2003)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Hanwen Yan
    • 1
  1. 1.Staples High SchoolWestportUSA

Personalised recommendations