DNA 2004: DNA Computing pp 399-409

An Inexpensive LED-Based Fluorometer Used to Study a Hairpin-Based DNA Nanomachine

  • Hanwen Yan
Part of the Lecture Notes in Computer Science book series (LNCS, volume 3384)


Fluorometers have become indispensable tools in the study of DNA-based nanomachines. The cost of such an instrument is usually outside the budget of a high school science department or that of an amateur scientist. This paper presents a low-cost fluorometer that can be assembled for the cost of approximately a hundred dollars. By monitoring Fluorescence Resonance Energy Transfer (FRET) from the donor dye TET to the acceptor dye TAMRA, this fluorometer has been successfully used to follow the repeated opening and closing of a DNA hairpin nanomachine. This instrument makes possible the investigation of DNA-based nanotechnology or the performance of FRET-based molecular biology experiments within a high school setting.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Hanwen Yan
    • 1
  1. 1.Staples High SchoolWestportUSA

Personalised recommendations