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Genome-Scale Modeling of Thermophilic Microorganisms

  • Sanjeev DahalEmail author
  • Suresh Poudel
  • R. Adam Thompson
Chapter
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 160)

Abstract

Thermophilic microorganisms are of increasing interest for many industries as their enzymes and metabolisms are highly efficient at elevated temperatures. However, their metabolic processes are often largely different from their mesophilic counterparts. These differences can lead to metabolic engineering strategies that are doomed to fail. Genome-scale metabolic modeling is an effective and highly utilized way to investigate cellular phenotypes and to test metabolic engineering strategies. In this review we chronicle a number of thermophilic organisms that have recently been studied with genome-scale models. The microorganisms spread across archaea and bacteria domains, and their study gives insights that can be applied in a broader context than just the species they describe. We end with a perspective on the future development and applications of genome-scale models of thermophilic organisms.

Keywords

Draft reconstruction Flux balance analysis Flux variability analysis Gap-filling Genome-scale models Stoichiometric matrix 

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Sanjeev Dahal
    • 1
    • 2
    • 3
    Email author
  • Suresh Poudel
    • 1
    • 2
    • 3
  • R. Adam Thompson
    • 3
    • 4
  1. 1.UT-ORNL Graduate School of Genome Science and TechnologyUniversity of TennesseeKnoxvilleUSA
  2. 2.Oak Ridge National LaboratoryOak RidgeUSA
  3. 3.BioEnergy Science CenterOak Ridge National LaboratoryOak RidgeUSA
  4. 4.Bredesen Center for Interdisciplinary Research and Graduate EducationUniversity of TennesseeKnoxvilleUSA

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