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A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies

  • Stefan Rödiger
  • Peter Schierack
  • Alexander Böhm
  • Jörg Nitschke
  • Ingo Berger
  • Ulrike Frömmel
  • Carsten Schmidt
  • Mirko Ruhland
  • Ingolf Schimke
  • Dirk Roggenbuck
  • Werner Lehmann
  • Christian SchröderEmail author
Chapter
First Online:
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 133)

Abstract

The analysis of different biomolecules is of prime importance for life science research and medical diagnostics. Due to the discovery of new molecules and new emerging bioanalytical problems, there is an ongoing demand for a technology platform that provides a broad range of assays with a user-friendly flexibility and rapid adaptability to new applications. Here we describe a highly versatile microscopy platform, VideoScan, for the rapid and simultaneous analysis of various assay formats based on fluorescence microscopic detection. The technological design is equally suitable for assays in solution, microbead-based assays and cell pattern recognition. The multiplex real-time capability for tracking of changes under dynamic heating conditions makes it a useful tool for PCR applications and nucleic acid hybridization, enabling kinetic data acquisition impossible to obtain by other technologies using endpoint detection. The paper discusses the technological principle of the platform regarding data acquisition and processing. Microbead-based and solution applications for the detection of diverse biomolecules, including antigens, antibodies, peptides, oligonucleotides and amplicons in small reaction volumes, are presented together with a high-content detection of autoimmune antibodies using a HEp-2 cell assay. Its adaptiveness and versatility gives VideoScan a competitive edge over other bioanalytical technologies.

Graphical Abstract

Keywords

Autoimmune diagnostic Hybridization kinetics Immunofluorescence patterns Microbeads Microscope imaging technology Multiplex Real-time 
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Notes

Acknowledgments

This work was supported by the BMBF InnoProfile-Projekt 03 JP 611. We thank the following people from Lausitz University of Applied Sciences: Jörg Weinreich, Andrea Krause, Katharina Metzner, Mandy Sowa, Claudia Deutschmann, and Alexander Kaiser. We are grateful to Reinhard Pregla, MD, Deutsches Herzzentrum Berlin for providing the human heart sample.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Stefan Rödiger
    • 1
    • 4
  • Peter Schierack
    • 1
  • Alexander Böhm
    • 1
  • Jörg Nitschke
    • 1
  • Ingo Berger
    • 1
  • Ulrike Frömmel
    • 1
  • Carsten Schmidt
    • 1
  • Mirko Ruhland
    • 1
  • Ingolf Schimke
    • 4
  • Dirk Roggenbuck
    • 3
  • Werner Lehmann
    • 2
  • Christian Schröder
    • 1
    Email author
  1. 1.Lausitz University of Applied SciencesSenftenbergGermany
  2. 2.Attomol GmbHLiptenGermany
  3. 3.MEDIPAN GmbHBerlinGermany
  4. 4.Medizinische Klinik (Kardiologie)Charité–Universitätsmedizin BerlinBerlinGermany

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