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Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells

  • Stefan Jakobs
  • Stefan Stoldt
  • Daniel Neumann
Chapter
Part of the Advances in Biochemical Engineering / Biotechnology book series (ABE, volume 124)

Abstract

Heterogeneity in the shapes of individual multicellular organisms is a daily experience. Likewise, even a quick glance through the ocular of a light microscope reveals the morphological heterogeneities in genetically identical cultured cells, whereas heterogeneities on the level of the organelles are much less obvious. This short review focuses on intracellular heterogeneities at the example of the mitochondria and their analysis by fluorescence microscopy. The overall mitochondrial shape as well as mitochondrial dynamics can be studied by classical (fluorescence) light microscopy. However, with an organelle diameter generally close to the resolution limit of light, the heterogeneities within mitochondria cannot be resolved with conventional light microscopy. Therefore, we briefly discuss here the potential of subdiffraction light microscopy (nanoscopy) to study inner-mitochondrial heterogeneities.

Graphical Abstract

Keywords

Fluorescence microscopy Mitochondria Nanoscopy Single-cell heterogeneity Super-resolution microscopy 

Abbreviations

GSD microscopy

Ground state depletion microscopy

GSDIM

Ground state depletion microscopy followed by individual molecule return

MMP

Mitochondrial membrane potential

PALM

Photoactivated localization microscopy

RESOLFT

Reversible saturable/switchable optical linear (fluorescence) transitions

STED microscopy

Stimulated emission depletion microscopy

STORM

Stochastic optical reconstruction microscopy

TOM

Translocase of the outer membrane

Notes

Acknowledgments

We thank C.A. Wurm for insightful discussions and providing some of the STED images. We also thank R. Schmidt and A. Egner regarding STED microscopy of mitochondria, S.W. Hell for continuous support and J. Jethwa for carefully reading the manuscript. Part of the work reported in this review was supported by the Bundesministerium für Bildung und Forschung (BMBF) (SysCompart, to S.J.).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Mitochondrial Structure and Dynamics GroupMax Planck Institute for Biophysical ChemistryGoettingenGermany

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