Whole Cell Sensing Systems I pp 109-130

Part of the Advances in Biochemical Engineering / Biotechnology book series (ABE, volume 117)

Surface Functionalization for Protein and Cell Patterning

  • Pascal Colpo
  • Ana Ruiz
  • Laura Ceriotti
  • François Rossi


The interaction of biological systems with synthetic material surfaces is an important issue for many biological applications such as implanted devices, tissue engineering, cell-based sensors and assays, and more generally biologic studies performed ex vivo. To ensure reliable outcomes, the main challenge resides in the ability to design and develop surfaces or artificial micro-environment that mimic ‘natural environment’ in interacting with biomolecules and cells without altering their function and phenotype. At this effect, microfabrication, surface chemistry and material science play a pivotal role in the design of advanced in-vitro systems for cell culture applications. In this chapter, we discuss and describe different techniques enabling the control of cell-surface interactions, including the description of some techniques for immobilization of ligands for controlling cell-surface interactions and some methodologies for the creation of well confined cell rich areas.


Surface chemistry patterning techniques microfabrication selfassembled monolayers plasma polymers soft lithography 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Pascal Colpo
    • 1
  • Ana Ruiz
    • 1
  • Laura Ceriotti
    • 1
  • François Rossi
    • 1
  1. 1.European Commission, Joint Research CentreInstitute for Health and Consumer ProtectionIspraItaly

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