Chapter

Optical Sensor Systems in Biotechnology

Volume 116 of the series Advances in Biochemical Engineering/Biotechnology pp 73-97

Date:

Optical Inline Measurement Procedures for Counting and Sizing Cells in Bioprocess Technology

  • Guido RudolphAffiliated withInstitut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover
  • , Patrick LindnerAffiliated withInstitut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover
  • , Arne BlumaAffiliated withInstitut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover
  • , Klaus JoerisAffiliated withBayer HealthCare
  • , Geovanni MartinezAffiliated withIPCV-LAB, IPCV-LAB, Escuela de Ingeniería Eléctrica, Universidad de Costa Rica
  • , Bernd HitzmannAffiliated withInstitut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover
  • , Thomas ScheperAffiliated withInstitut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover Email author 

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Abstract

To observe and control cultivation processes, optical sensors are used increasingly. Important parameters for controlling such processes are cell count, cell size distribution, and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process parameters. A disadvantage of most previously developed imaging procedures is that they are only available offline which requires sampling. On the other hand, available imaging inline probes can so far only deliver a limited number of process parameters. This chapter presents new optical procedures for the inline determination of cell count, cell size distribution, and other parameters. In particular, by in situ microscopy an imaging procedure will be described which allows the determination of direct and nondirect cell parameters in real time without sampling.

Keywords

Cell size distribution Focused beam reflectance measurement (FBRM) In situ microscopy Inline monitoring Optical sensors