Optical Inline Measurement Procedures for Counting and Sizing Cells in Bioprocess Technology

  • Guido Rudolph
  • Patrick Lindner
  • Arne Bluma
  • Klaus Joeris
  • Geovanni Martinez
  • Bernd Hitzmann
  • Thomas Scheper
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 116)


To observe and control cultivation processes, optical sensors are used increasingly. Important parameters for controlling such processes are cell count, cell size distribution, and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process parameters. A disadvantage of most previously developed imaging procedures is that they are only available offline which requires sampling. On the other hand, available imaging inline probes can so far only deliver a limited number of process parameters. This chapter presents new optical procedures for the inline determination of cell count, cell size distribution, and other parameters. In particular, by in situ microscopy an imaging procedure will be described which allows the determination of direct and nondirect cell parameters in real time without sampling.


Cell size distribution Focused beam reflectance measurement (FBRM) In situ microscopy Inline monitoring Optical sensors 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Guido Rudolph
    • 1
  • Patrick Lindner
    • 1
  • Arne Bluma
    • 1
  • Klaus Joeris
    • 2
  • Geovanni Martinez
    • 3
  • Bernd Hitzmann
    • 1
  • Thomas Scheper
    • 1
  1. 1.Institut für Technische ChemieGottfried Wilhelm Leibniz Universität HannoverHannoverGermany
  2. 2.Bayer HealthCareBerkeleyUSA
  3. 3.IPCV-LABIPCV-LAB, Escuela de Ingeniería Eléctrica, Universidad de Costa RicaSan JoséUSA

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