Optical Inline Measurement Procedures for Counting and Sizing Cells in Bioprocess Technology

  • Guido Rudolph
  • Patrick Lindner
  • Arne Bluma
  • Klaus Joeris
  • Geovanni Martinez
  • Bernd Hitzmann
  • Thomas Scheper
Chapter

Abstract

To observe and control cultivation processes, optical sensors are used increasingly. Important parameters for controlling such processes are cell count, cell size distribution, and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process parameters. A disadvantage of most previously developed imaging procedures is that they are only available offline which requires sampling. On the other hand, available imaging inline probes can so far only deliver a limited number of process parameters. This chapter presents new optical procedures for the inline determination of cell count, cell size distribution, and other parameters. In particular, by in situ microscopy an imaging procedure will be described which allows the determination of direct and nondirect cell parameters in real time without sampling.

Keywords

Cell size distribution Focused beam reflectance measurement (FBRM) In situ microscopy Inline monitoring Optical sensors 

References

  1. 1.
    Konstantinov K, Chuppa S, Sajan E, Tsai Y, Yoon SJ, Golini F (1994) Trends Biotechnol 12:324CrossRefGoogle Scholar
  2. 2.
    Harris CM, Kell DB (1985) Biosensors 1:17CrossRefGoogle Scholar
  3. 3.
    Kell DB, Markx GH, Davey CL, Todd RW (1990) Trends Anal Chem 9:190CrossRefGoogle Scholar
  4. 4.
    Sonnleitner B, Locher G, Fiechter A (1992) J Biotechnol 25:5CrossRefGoogle Scholar
  5. 5.
    Barrett P, Glennon B (1999) Part Part Syst Charact 16:207CrossRefGoogle Scholar
  6. 6.
    Heath AR, Fawell PD, Bahri PA, Swift JD (2002) Part Part Syst Charact 19:84CrossRefGoogle Scholar
  7. 7.
    Heffels C, Polke R, Radle M, Sachweh B, Schafer M, Scholz N (1998) Part Part Syst Charact 15:211CrossRefGoogle Scholar
  8. 8.
    Tadayyon A, Rohani S (1998) Part Part Syst Charact 15:127CrossRefGoogle Scholar
  9. 9.
    Schirg P, Wissler P (2001) Chem Ing Tech 377Google Scholar
  10. 10.
    De Clercq B, Lant PA, Vanrolleghem PA (2004) J Chem Technol Biotechnol 79:610CrossRefGoogle Scholar
  11. 11.
    Simmons MJH, Azzopardi BJ (2001) Int J Multiphase Flow 27:843CrossRefGoogle Scholar
  12. 12.
    Pearson AP, Glennon B, Kieran PM (2003) Biotechnol Progr 19:1342CrossRefGoogle Scholar
  13. 13.
    Pearson AP, Glennon B, Kieran PM (2004) J Chem Technol Biotechnol 79:1142CrossRefGoogle Scholar
  14. 14.
    Jeffers P, Raposo S, Lima-Costa ME, Connolly P, Glennon B, Kieran PM (2003) Biotechnol Lett 25:2023CrossRefGoogle Scholar
  15. 15.
    Jeffers PT, Raposo S, Lima-Costa ME, Kieran P, Glennon B (2003) Abstr Paper Am Chem Soc 225:U188Google Scholar
  16. 16.
    Konan (1990) Technical Report. Konan Camera Research Institute, Hyogo, JapanGoogle Scholar
  17. 17.
    Kumada J, Takahashi T, Nakatani K, Nagami K (1981) 18th European brewery convention (EBC). Copenhagen, Denmark, p 293Google Scholar
  18. 18.
    Suhr H, Speil P, Wehnert G, Storhas W (1991) German Patent, DE 40,32,002Google Scholar
  19. 19.
    Suhr H-, Wehnert G, Schneider K, Bittner C, Scholz T, Geissler P, Jähne B, Scheper T (1995) Biotechnol Bioeng 47:106Google Scholar
  20. 20.
    Schneider K (1995) PhD thesis, University of Hannover, GermanyGoogle Scholar
  21. 21.
    Scholz T (1995) PhD thesis, University of Heidelberg, GermanyGoogle Scholar
  22. 22.
    Barrett P, Glennon B (2002) Chem Eng Res Des 80:799CrossRefGoogle Scholar
  23. 23.
    Kougoulos E, Jones AG, Jennings KH, Wood-Kaczmar MW (2005) J Cryst Growth 273:529CrossRefGoogle Scholar
  24. 24.
    O'Sullivan B, Barrett P, Hsiao G, Carr A, Glennon B (2003) Org Process Res Dev 7:977CrossRefGoogle Scholar
  25. 25.
    Bittner C (1994) PhD thesis, University of Hannover, GermanyGoogle Scholar
  26. 26.
    Bittner C, Wehnert G, Scheper T (1998) Biotechnol Bioeng 60:24CrossRefGoogle Scholar
  27. 27.
    Frerichs JG (2000) PhD thesis, University of Hannover, GermanyGoogle Scholar
  28. 28.
    Frerichs JG, Joeris K, Konstantinov K, Scheper T (2002) Chem Ing Tech 74:1629CrossRefGoogle Scholar
  29. 29.
    Joeris K, Frerichs JG, Konstantinov K, Scheper T (2002) Cytotechnology 38:129CrossRefGoogle Scholar
  30. 30.
    Martinez G, Frerichs JG, Joeris K, Kontantinov K, Scheper T (2005) IEEE international conference on acoustics, speech and signal processing (ICASSP), Philadelphia, PA, USA, p 497Google Scholar
  31. 31.
    Brückerhoff T, Frerichs JG, Joeris K, Konstantinov K, Scheper T (2005) In: Godia F, Fussenegger M (eds) Animal cell technology meets genomics. Proceedings of the 18th ESACT meeting, Granada, Spain, May 11–14, 2003. Springer, Dordrecht, p 589CrossRefGoogle Scholar
  32. 32.
    Brückerhoff T (2006) PhD thesis, University of Hannover, GermanyGoogle Scholar
  33. 33.
    Larsson C, Vonstockar U, Marison I, Gustafsson L (1993) J Bacteriol 175:4809CrossRefGoogle Scholar
  34. 34.
    Camisard V, Brienne JP, Baussart H, Hammann J, Suhr H (2002) Biotechnol Bioeng 78:73CrossRefGoogle Scholar
  35. 35.
    Guez JS, Cassar JP, Wartelle F, Dhulster P, Suhr H (2004) J Biotechnol 111:335CrossRefGoogle Scholar
  36. 36.
    Hetland G, Ohno N, Aaberge IS, Lovik M (2000) FEMS Immunol Med Microbiol 27:111Google Scholar
  37. 37.
    Ohno N, Miura NN, Nakajima M, Yadomae T (2000) Biol Pharm Bull 23:866CrossRefGoogle Scholar
  38. 38.
    Rudolph G, Lindner P, Gierse A, Bluma A, Martinez G, Hitzmann B, Scheper T (2007) Biotechnol BioengGoogle Scholar
  39. 39.
    Martinez G (2005) IAPR Conference on machine vision applications (MVA), Tsukuba, Japan, p 476Google Scholar
  40. 40.
    Espinoza E, Martinez G, Frerichs J-G, Scheper T (2006) 3rd IEEE international symposium on biomedical imaging, Arlington, Virginia, USA, p 542Google Scholar
  41. 41.
    Smith S, Bradly J (1997) Int J Comput Vis 23:45Google Scholar
  42. 42.
    Martinez G, Frerichs JG, Joeris K, Konstantinov K, Scheper T (2006) IEEE international conference of acoustics, speech and signal processing (ICASSAP), Tolouse, France, p 581Google Scholar
  43. 43.
    Bichsel M, Pentland P (1992) IEEE international conference on computer vision and pattern recognition, p 459Google Scholar
  44. 44.
    Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007) Biotechnol Lett 29:373CrossRefGoogle Scholar
  45. 45.
    Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007) Biotechnol Bioeng 97:1489CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Guido Rudolph
    • 1
  • Patrick Lindner
    • 1
  • Arne Bluma
    • 1
  • Klaus Joeris
    • 2
  • Geovanni Martinez
    • 3
  • Bernd Hitzmann
    • 1
  • Thomas Scheper
    • 1
  1. 1.Institut für Technische ChemieGottfried Wilhelm Leibniz Universität HannoverHannoverGermany
  2. 2.Bayer HealthCareBerkeleyUSA
  3. 3.IPCV-LABIPCV-LAB, Escuela de Ingeniería Eléctrica, Universidad de Costa RicaSan JoséUSA

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