Large Scale Production of Stem Cells and Their Derivatives

Chapter
Part of the Advances in Biochemical Engineering / Biotechnology book series (ABE, volume 114)

Abstract

Stem cells have been envisioned to become an unlimited cell source for regenerative medicine. Notably, the interest in stem cells lies beyond direct therapeutic applications. They might also provide a previously unavailable source of valuable human cell types for screening platforms, which might facilitate the development of more efficient and safer drugs. The heterogeneity of stem cell types as well as the numerous areas of application suggests that differential processes are mandatory for their in vitro culture. Many of the envisioned applications would require the production of a high number of stem cells and their derivatives in scalable, well-defined and potentially clinical compliant manner under current good manufacturing practice (cGMP). In this review we provide an overview on recent strategies to develop bioprocesses for the expansion, differentiation and enrichment of stem cells and their progenies, presenting examples for adult and embryonic stem cells alike.

Keywords

Bioreactor Cell therapy Differentiation Process development Stem cells Teratoma 

Abbreviations

(NOD/SCID)

miceNonobese diabetic/severe combined immunodeficient

(RWV)

bioreactorRotating wall vessel

bFGF, FGF-2

Basic fibroblast growth factor

BM

Bone marrow

BMP

Bone morphogenetic protein

BMP

Morphogenetic protein

cGMP

Current good manufacturing practice

CHO

Hamster ovary cells

EBs

Embryoid bodies

ESC

Embryonic stem cells

GM-CSF

Granulocyte macrophage colony stimulating factor

hESC

Human embryonic stem cell

hNPC

Neural precursor cells

HSC

Hematopoietic stem and progenitor cells

LIF

Leukemia inhibitory factor bone

MASC

Magnetic activated cell sorting

MI

Myocardial infarction

MSC

Mesenchymal stem cells

NSC

Neural stem cells

PB

(Mobilized) Peripheral blood

SCF

Stem cell factor

SNM`

Spherical neural masses

TGF-beta

Transforming growth factor beta

UCB

Umbilical cord blood

Notes

Acknowledgements

I thank Blaine Phillips, William Rust, Birgit Andree, Harmeet Singh, Zhou WeiZhuang (Institute of Medical Biology, Singapore) and Andre Choo (Bioprocessing Technology Institute, Singapore) for helpful comments and a critical review of this manuscript.

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© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Institute of Medical Biology (IMB)SingaporeSingapore

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