Large Scale Production of Stem Cells and Their Derivatives

Part of the Advances in Biochemical Engineering / Biotechnology book series (ABE, volume 114)


Stem cells have been envisioned to become an unlimited cell source for regenerative medicine. Notably, the interest in stem cells lies beyond direct therapeutic applications. They might also provide a previously unavailable source of valuable human cell types for screening platforms, which might facilitate the development of more efficient and safer drugs. The heterogeneity of stem cell types as well as the numerous areas of application suggests that differential processes are mandatory for their in vitro culture. Many of the envisioned applications would require the production of a high number of stem cells and their derivatives in scalable, well-defined and potentially clinical compliant manner under current good manufacturing practice (cGMP). In this review we provide an overview on recent strategies to develop bioprocesses for the expansion, differentiation and enrichment of stem cells and their progenies, presenting examples for adult and embryonic stem cells alike.


Bioreactor Cell therapy Differentiation Process development Stem cells Teratoma 



miceNonobese diabetic/severe combined immunodeficient


bioreactorRotating wall vessel


Basic fibroblast growth factor


Bone marrow


Bone morphogenetic protein


Morphogenetic protein


Current good manufacturing practice


Hamster ovary cells


Embryoid bodies


Embryonic stem cells


Granulocyte macrophage colony stimulating factor


Human embryonic stem cell


Neural precursor cells


Hematopoietic stem and progenitor cells


Leukemia inhibitory factor bone


Magnetic activated cell sorting


Myocardial infarction


Mesenchymal stem cells


Neural stem cells


(Mobilized) Peripheral blood


Stem cell factor


Spherical neural masses


Transforming growth factor beta


Umbilical cord blood



I thank Blaine Phillips, William Rust, Birgit Andree, Harmeet Singh, Zhou WeiZhuang (Institute of Medical Biology, Singapore) and Andre Choo (Bioprocessing Technology Institute, Singapore) for helpful comments and a critical review of this manuscript.


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© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Institute of Medical Biology (IMB)SingaporeSingapore

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