Volume 108 of the series Advances in Biochemical Engineering/Biotechnology pp 205-235


Consolidated Bioprocessing for Bioethanol Production Using Saccharomyces cerevisiae

  • Willem H. van ZylAffiliated withDepartment of Microbiology, Stellenbosch University Email author 
  • , Lee R. LyndAffiliated withThayer School of Engineering, Dartmouth College
  • , Riaan den HaanAffiliated withDepartment of Microbiology, Stellenbosch University
  • , John E. McBrideAffiliated withThayer School of Engineering, Dartmouth College

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Consolidated bioprocessing (CBP) of lignocellulose to bioethanol refers to the combining of the four biological events required for this conversion process (production of saccharolytic enzymes, hydrolysis of the polysaccharides present in pretreated biomass, fermentation of hexose sugars, and fermentation of pentose sugars) in one reactor. CBP is gaining increasing recognition as a potential breakthrough for low-cost biomass processing. Although no natural microorganism exhibits all the features desired for CBP, a number of microorganisms, both bacteria and fungi, possess some of the desirable properties. This review focuses on progress made toward the development of baker's yeast (Saccharomyces cerevisiae) for CBP. The current status of saccharolytic enzyme (cellulases and hemicellulases) expression in S. cerevisiae to complement its natural fermentative ability is highlighted. Attention is also devoted to the challenges ahead to integrate all required enzymatic activities in an industrial S. cerevisiae strain(s) and the need for molecular and selection strategies pursuant to developing a yeast capable of CBP.
Consolidated bioprocessing Cellulolytic yeast One-step bioethanol production Saccharomyces cerevisiae