- Jean-Louis ReymondAffiliated withDepartment of Chemistry & Biochemistry, University of Berne Email author
- , Peter BabiakAffiliated withDepartment of Chemistry & Biochemistry, University of Berne
Enzyme screening technology has undergone massive developments in recent years, particularly in the area of high-throughput screening and microarray methods. Screening consists of testing each sample of a sample library individually for the targeted reaction. This requires enzyme assays that accurately test relevant parameters of the reaction, such as catalytic turnover with a given substrate and selectivity parameters such as enantio- and regioselectivity. Enzyme assays also play an important role outside of enzyme screening, in particular for drug screening, medical diagnostics, and in the area of cellular and tissue imaging.
In the 1990s, methods for high-throughput screening of enzyme activities were perceived as a critical bottleneck. As illustrated partly in this chapter, a large repertoire of efficient screening strategies are available today that allow testing of almost any reaction with high-throughput.
- Screening Systems
- Book Title
- White Biotechnology
- pp 31-58
- Print ISBN
- Online ISBN
- Series Title
- Advances in Biochemical Engineering/Biotechnology
- Series Volume
- Series ISSN
- Springer Berlin Heidelberg
- Copyright Holder
- Springer-Verlag Berlin Heidelberg
- Additional Links
- Industry Sectors
- eBook Packages
To view the rest of this content please follow the download PDF link above.