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Gene Expression Profiles of Mesenchymal Stem Cells

  • D. G. Phinney

6. Concluding Remarks

Past studies profiling gene expression in MSCs have typically revealed a preponderance of expressed transcripts encoding structural proteins common in skeletal tissue and secreted factors that regulate hematopoiesis. Our SAGE analysis of the MSC transcriptome corroborated many of these previous findings, but also revealed that the cells express a plethora of transcripts encoding proteins involved in cell communication, motility, neural activity, angiogenesis and other biological processes that characterize marrow stroma. Therefore, the heterogeneity of the MSC transcriptome appears to reflect the nature and function of the different cell types that comprise this organ, the complexity of which is under appreciated. Our studies also indicate that the transcriptome of a clonal MSC population is characterized by a high percentage of expressed transcripts encoding proteins of an indeterminate nature. These findings reflect a basic lack of knowledge regarding the biology of MSCs, which attributes to the difficulty in ascribing a molecular phenotype to these stem cells. Comparative genomics studies indicate that transcripts uniquely expressed in MSCs include transcription factors and signalling molecules involved in limb bud morphogenesis, thereby providing clues to the regulatory mechanisms governing self-renewal and lineage commitment of MSCs. Deciphering these molecular pathways will further our understanding of the nature and biology of this unique stem cell population.

Keywords

Stromal Cell Mesenchymal Stem Cell Bone Marrow Stromal Cell Human Mesenchymal Stem Cell Human MSCs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2006

Authors and Affiliations

  • D. G. Phinney
    • 1
  1. 1.Centre for Gene TherapyTulane University Health Sciences CenterNew Orleans

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