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Process Optimization for an NS0-Derived Hybridoma Cell Line in a Chemically Defined, Protein-Free Medium

  • J. M. Johnson
  • J. Hartshorn
  • S. Mcnorton
  • J. A. Padilla
  • T. Mohabbat
  • S. Luo
  • K. Etchberger
Conference paper
Part of the ESACT Proceedings book series (ESACT, volume 2)

Abstract

A process optimization study was performed in stirred-tank bioreactors to determine parameter set points that maximize cell growth, viability, and monoclonal antibody (MAb) production. A statistical design of experiment (DoE) software package was used to determine parameter settings for dissolved oxygen (DO), pH, and temperature. An immunoglobulin G (IgG) producing NS0-derived hybridoma cell line was adapted to growth in a chemically defined, protein-free and animal-component free medium. Growth and productivity were characterized at the 2L bioreactor scale. Results were analyzed based on variable responses including growth and productivity on a per-liter basis. Parameter ranges that yield optimal productivity were determined.

Keywords

Dissolve Oxygen Maximize Cell Growth Animal Cell Technology Peak Cell Density Analytical Method Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2005

Authors and Affiliations

  • J. M. Johnson
    • 1
  • J. Hartshorn
    • 1
  • S. Mcnorton
    • 1
  • J. A. Padilla
    • 1
  • T. Mohabbat
    • 1
  • S. Luo
    • 1
  • K. Etchberger
    • 1
  1. 1.JRH Biosciences, Inc.LenexaUSA

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